To elucidate the biological influence of space radiation, we studied the effects of long-term space flight on mutation of the bacterial ribosomal protein L gene (rpsL). We prepared dried samples of plasmid DNA and repair-deficient and wild type cells of Escherichia (E.) coli.
View Article and Find Full Text PDFDolichyl monophosphate (Dol-P) is involved in the attachment of carbohydrate chains to proteins in the formation of N-linked glycoprotein. We found that this compound induces apoptosis in human leukemia U937 cells. During this apoptotic execution, the increase of plasma membrane fluidity (5-20 min), reduction in mitochondrial transmembrane potential (delta psi m) and translocation of apoptosis-inducing factor (1-3 hr), caspase-3-like protease activation (2-4 hr), chromatin condensation and DNA ladder formation (3-4 hr) were observed successively.
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