Publications by authors named "Tsugio Amemiya"

Purpose: The purpose of this study was to demonstrate fatty acids in the rat retina and to show that time-of-flight secondary ion mass spectrometry (TOF-SIMS) can replace histochemistry.

Methods: Five normal 3-month-old Wistar Kyoto rats were used. The eyes were enucleated under pentobarbital anesthesia, immediately put into liquid nitrogen without any fixation, then cut into semithin sections, which were covered by OCT compound.

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Purpose: To examine the conjunctiva and cornea of riboflavin-deficient rats with scanning (SEM) and transmission (TEM) electron microscopes.

Materials And Methods: Three-week-old Wistar Kyoto rats were fed a riboflavin-deficient diet (0.05 mg riboflavin/100 g) for 3 months.

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Purpose: We studied the cornea and conjunctiva of Zn-deficient rats with an electron microscope and time-of-flight secondary ion mass spectrometry (TOF-SIMS) to elucidate the role of trace elements in the cornea and conjunctiva.

Materials And Methods: Twenty-one-day-old Wistar Kyoto rats were fed a Zn-deficient diet and deionized water for 7 weeks and then killed. The control rats were fed a Zn-deficient diet and deionized water supplemented with 3 mg Zn/100 ml.

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Background: Patients with adult T-cell leukemia (ATL) may have eyelid lymphoma, uveitis, or cytomegalovirus retinitis due to being immunocompromised. However, there have been few reports on the invasion of multiple ocular lesions. We treated 1 unusual ATL patient with uveitis in whom multiple ocular invasions were suspected.

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Purpose: To determine whether FR118487, a recently developed angiogenesis inhibitor, affects experimental choroidal neovascularization (CNV) induced by laser photocoagulation in pigmented rats.

Methods: Focal laser photocoagulation (argon green 50 mW, 0.04 seconds, 200 microm) was applied to the retinochoroid of normal Brown Norway rats.

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Background: This report describes the pathology of myolipoma, which is a benign soft tissue tumor and is a very rare tumor of the orbit.

Methods: A 41-year-old woman complained of exopthalmos of her right eye. By MRI (magnetic resonance imaging) examination, the tumor (30x20 mm) was located in the extraconus of four recti muscles.

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We examined with time-of-flight secondary-ion mass spectrometry (TOF-SIMS) various substances in vitreous proliferative tissues excised from patients with proliferative diabetic retinopathy or proliferative vitreoretinopathy. Amounts of elements, vitamins and fatty acids were measured with TOF-SIMS and then the average ratio of the count of spattered ions of each element and organic substance to the total count of spattered ions was calculated. The average ratios of Fe, Ca, Al, Zn and Cu ion counts to the total count of spattered ions were significantly larger in proliferative diabetic retinopathy than in proliferative vitreoretinopathy.

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Background: Although the tubulointerstitial nephritis and uveitis (TINU) syndrome has been reported, there are only a few reports in the ophthalmological literature. The ocular findings usually appear later than the renal findings, and a renal biopsy is required for a definitive diagnosis.

Cases: Case 1 was a 15-year-old Japanese girl who was a carrier of the human T lymphotropic virus, type 1 (HTLV-1).

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Purpose: The purpose of the current study is to investigate the cornea in magnesium (Mg) deficiency and elucidate the local function of trace elements.

Methods: After delivery, mother Wistar Kyoto rats were fed a low Mg diet containing 0.1 mg Mg/100 g diet with all other nutrients and distilled and deionized water.

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Purpose: To determine whether vitamin E can prevent changes in the cornea and conjunctiva due to vitamin A deficiency.

Methods: Male infant rats from normal mothers were weaned at 20 days of age and divided into three groups: in group 1, A(-), rats were fed a vitamin A-deficient diet; in group 2, A(-)E(+), rats were fed a vitamin A-deficient diet and injected intraperitoneally with 15 mg of vitamin E per week; group 3 rats were normal controls. At 13 weeks of age, the corneal and conjunctival changes were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

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Purpose: To determine whether irsogladine inhibits experimental choroidal neovascularization (CNV) induced by laser photocoagulation in pigmented rats.

Methods: Focal laser photocoagulation (argon green 50 mW, 0.04 s, 200 microm) was applied to the retinochoroid of normal Brown Norway rats.

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Purpose: The ultrastructure of the optic nerves in magnesium (Mg)-deficient rats was studied to elucidate the function of Mg.

Methods: After delivery, mother Wistar Kyoto rats were fed a low-Mg diet containing 0.1 mg Mg/100 g diet with all other nutrients and distilled and deionized water.

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The upper and lower eyelids fuse together during development and then reseparate. The mechanism of reseparation of the eyelids is still unclear, although many reports agree that keratinization has a major role in lid separation. We applied apoptotic detection methods with ultrastructural features to examine the process of lid separation in rats.

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Purpose: To study retinal changes in the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a spontaneously diabetic rat, and evaluate it as a possible new diabetic retinopathy model.

Methods: We examined the retinas of OLETF rats and Long-Evans Tokushima Otsuka (LETO) rats as controls under both a transmission electron microscope (TEM) and a scanning electron microscope (SEM).

Results: We detected higher blood sugar level in the OLETF rats than in the LETO rats beginning at 5 months of age.

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Purpose: The purpose of this study was to assess morphologically the interaction between zinc and vitamin A in their effect on the ocular surface.

Methods: Three-week-old Wistar Kyoto rats were divided into five groups: group A(+) Zn(+) was fed a diet containing both vitamin A and Zn; group A(-) was fed a vitamin A-deficient diet; group A(-) Zn(+) was given a vitamin A-deficient diet and deionized distilled water with Zn; group Zn(-) and group Zn(-) A(+) were fed a Zn-deficient diet, whereby group Zn(-) A(+) received an intraperitoneal injection of vitamin A. Corneas and conjunctivas of each group were examined by electron microscopy.

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The long-term effects of the angiotensin-converting enzyme (ACE) inhibitor cilazapril on retinal and choroidal circulation in rats with spontaneous diabetes type 2 were assessed by corrosion casts, scanning electron microscopy (SEM) and transmission electron microscopy. One group of 20 male Otsuka Long-Evans Tokushima Fatty (OLETF) rats was treated with 10 mg/kg/day of cilazapril from 4 to 64 weeks of age, and 20 other OLETF rats received no treatment. A third group, 20 male Long-Evans Tokushima Otsuka (LETO) rats, served as age-matched controls.

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Objectives: The details of the morphological features of choroidal neovascularization (CNV) remain unclear. The purpose of this study was to establish a CNV rat model and study the natural course of CNV using vascular casts and a scanning electron microscope (SEM).

Methods: Focal laser photocoagulation (argon green 50 mW, 0.

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The purpose of this study is to investigate the effect of Cr deficiency on the rat retina. Three-week-old Wistar Kyoto rats were divided into 2 groups. Cr-deficient rats were fed AIN-93G diet without Cr and deionized distilled water.

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Purpose: The purpose of this study is to clarify the relationship between retinal vascular obstruction and asymptomatic cerebral infarction (ACI).

Methods: Forty-three patients (27 men and 16 women) with retinal vascular obstruction were examined by magnetic resonance imaging (MRI) at the Department of Ophthalmology of Nagasaki University Hospital in Nagasaki, Japan. Patients with a history of neurological signs and symptoms were excluded from this study.

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Background And Objective: One of the serious complications that may arise after macular hole (MH) surgery is a temporal visual fields (TVF) defect. We hypothesized that hyperbaric oxygen (HBO) therapy improves the visual field (VF) in these patients.

Materials And Methods: Vitrectomy for MH was performed on 73 eyes from 1994 to 1997.

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Purpose: The purpose of the present study was to elucidate the specific morphological changes of the choroidal vasculature in long-term hypertension.

Methods: The choroidal vessels of spontaneously hypertensive (SHR) rats were examined with corrosion casts/scanning electron microscope (SEM) and the transmission electron microscope (TEM).

Results: In 18-month-old SHRs, corrosion casts and SEM showed tortuosity, caliber irregularity and generalized narrowing of the choroidal arteries.

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We studied rat retinal changes due to aluminum (Al) toxicosis with a transmission electron microscope (TEM) and an energy dispersive X-ray analyzer (EDXA). Normal 4-week-old Wistar Kyoto rats were divided randomly into Al toxicosis and control groups. The Al toxicosis group was injected ip with 0.

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A review of reports on metastatic orbital tumors published from 1903 to 1998 in Japan revealed 128 patients, 74 males, 52 females and 2 whose sex was not recorded. The average age was 44.8 years, but varied depending on the primary tumor.

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