Publications by authors named "Tsering Yangzom"
Article Synopsis
- Mitochondrial diseases cause neuronal death and depletion of mitochondrial DNA (mtDNA), with astrocytes potentially playing a damaging role in neurodegeneration.
- Research using induced pluripotent stem cells (iPSCs) from patients with POLG mutations showed that the resulting astrocytes experienced significant mitochondrial dysfunction and developed a toxic phenotype.
- When these dysfunctioning astrocytes interacted with neurons, they induced neuronal death, highlighting a novel toxic contribution of astrocytes to the progression of POLG-related diseases.
Article Synopsis
- A 3D brain organoid model is created to investigate POLG-related encephalopathy, a mitochondrial disease caused by POLG mutations, using patient-derived induced pluripotent stem cells (iPSCs).
- The generated cortical organoids display key disease features like abnormal structure, neuron loss, and depleted mitochondrial DNA, along with changes in critical neuronal development pathways and increased NOTCH and JAK-STAT signaling.
- Metformin treatment improved several issues found in the organoids, but did not resolve problems with inhibitory dopamine-glutamate neurons, making this model a useful tool for studying POLG disorders and related diseases linked to mitochondrial dysfunction.
Article Synopsis
- Alpers' syndrome is a severe neurodegenerative disorder often caused by mutations in the POLG gene, leading to issues like intractable epilepsy and developmental regression, with no current effective treatments.
- Researchers created patient-specific induced pluripotent stem cells (iPSCs) from an Alpers' patient to study neural dysfunction and observed that organoids replicated key molecular changes seen in actual patient brain tissue.
- The study found that the NAD precursor nicotinamide riboside (NR) improved mitochondrial function, suggesting it could be a potential treatment for Alpers' syndrome and other similar mitochondrial disorders.*
Cardiac rhythm regulated by micro-macroscopic structures of heart. Pacemaker abnormalities or disruptions in electrical conduction, lead to arrhythmic disorders may be benign, typical, threatening, ultimately fatal, occurs in clinical practice, patients on digitalis, anaesthesia or acute myocardial infarction. Both traditional and genetic animal models are: In-vitro: Isolated ventricular Myocytes, Guinea pig papillary muscles, Patch-Clamp Experiments, Porcine Atrial Myocytes, Guinea pig ventricular myocytes, Guinea pig papillary muscle: action potential and refractory period, Langendorff technique, Arrhythmia by acetylcholine or potassium.
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- * Researchers have developed a flow cytometry method to analyze multiple mitochondrial characteristics in brain organoids created from human induced pluripotent stem cells (iPSCs).
- * The provided protocol allows for efficient generation and analysis of cortical organoids, enabling the assessment of key mitochondrial proteins and the indirect measurement of mitochondrial DNA copy numbers within a few hours.
Bloodstream infections (BSIs) are one of the frequent nosocomial infections among hospitalized patients. To understand the local epidemiology and evolving antimicrobial drug resistance of blood-borne pathogens, we analyzed the distribution and antibiotic sensitivity profile of organisms causing BSI in our hospital-based study. We reviewed retrospective data of laboratory-confirmed BSIs, from January 2013 to December 2018.
View Article and Find Full Text PDFBackground: Enterococcus is an important pathogen, and with its emergence of resistance to multiple antimicrobials, the management of infection is becoming increasingly difficult.
Aim: The aim of the study is to determine the prevalence, antibiotic resistance, and risk factors associated with enterococcal infection or colonization.
Materials And Methods: In this prospective study, samples from inpatients were screened for resistant enterococci.