Thermal Imaging and Dimensionality Reduction Techniques for Subclinical Mastitis Detection in Dairy Sheep.

Subclinical mastitis is a common and economically significant disease that affects dairy sheep production. Thermal imaging presents a promising avenue for non-invasive detection, but existing methodologies often rely on simplistic temperature differentials, potentially leading to inaccurate assessments. This study proposes an advanced algorithmic approach integrating thermal imaging processing with statistical texture analysis and t-distributed stochastic neighbor embedding (t-SNE).

Physical unclonable functions on carbon fiber reinforced polymers using laser techniques.

We have successfully fabricated physical unclonable functions (PUF)s on carbon fiber reinforced polymers (CFRP) in the form of computer-generated holograms (CGH)s using an industrial friendly UV ns laser system. The topological randomness of the fiber network and laser absorption yield laser engraved CGH that are unique. The engraved CGH serve as PUFs and this is confirmed by the exceptionally low values of probability of cloning (POC), lower than 10, well below the commonly accepted threshold value of 10 in the literature.

Probing the Fucoxanthin-Chlorophyll /-Binding Proteins (FCPs) of the Marine Diatom sp. by Resonance Raman Spectroscopy.

We report resonance Raman spectra of the light-harvesting fucoxanthin-chlorophyll /-binding proteins (FCPs) of marine diatom sp. The Raman shifts in the N-isotope-enriched diatom provide the first spectroscopic evidence for the characterization of the C-N marker bands and, thus, of the penta- and hexacoordinated states of chlorophylls / in the FCPs. Under 405 and 442 nm Raman excitations, all of the marker bands of Chl / are observed and the isotope-based assignments provide new information concerning the structure of Chls / in the FCPs and their interactions with the protein environment.

Reversible thermally induced spin crossover in the myoglobin-nitrito adduct directly monitored by resonance Raman spectroscopy.

Myoglobin has been demonstrated to function as a nitrite reductase to produce nitric oxide during hypoxia. One of the most intriguing aspects of the myoglobin/nitrite interactions revealed so far is the unusual O-binding mode of nitrite to the ferric heme iron, although conflicting data have been reported for the electronic structure of this complex also raising the possibility of linkage isomerism. In this work, we applied resonance Raman spectroscopy in a temperature-dependent approach to investigate the binding of nitrite to ferric myoglobin and the properties of the formed adduct from ambient to low temperatures (293 K to 153 K).

Microstructure-driven annihilation effects and dispersive excited state dynamics in solid-state films of a model sensitizer for photon energy up-conversion applications.

Bimolecular processes involving exciton spin-state interactions gain attention for their deployment as wavelength-shifting tools. Particularly triplet-triplet annihilation induced photon energy up-conversion (TTA-UC) holds promise to enhance the performance of solar cell and photodetection technologies. Despite the progress noted, a correlation between the solid-state microstructure of photoactuating TTA-UC organic composites and their photophysical properties is missing.

Evidence for reversible light-dependent transitions in the photosynthetic pigments of diatoms.

Marine diatoms contribute to oxygenic photosynthesis and carbon fixation and handle large changes under variable light intensity on a regular basis. The unique light-harvesting apparatus of diatoms are the fucoxanthin-chlorophyll /-binding proteins (FCPs). Here, we show the enhancement of chlorophyll / (Chl /), fucoxanthin (Fx), and diadinoxanthin (Dd) marker bands in the Raman spectra of the centric diatom , which allows distinction of the pigment content in the cells grown under low- (LL) and high-light (HL) intensity at room temperature.

Melding Fog Computing and IoT for Deploying Secure, Response-Capable Healthcare Services in 5G and Beyond.

The fifth generation (5G) of mobile networks is designed to mark the beginning of the hyper-connected society through a broad set of novel features and disruptive characteristics, delivering massive connectivity, coverage and availability paired with unprecedented speed, throughput and capacity. Such a highly capable networking paradigm, facilitated by its integrated segments and available subsystems, will propel numerous cutting-edge, innovative and versatile services, spanning every possible business vertical. Augmented, response-capable healthcare services have already been identified as one of the prime objectives of both vendors and customers; therefore, addressing controversies and shortcomings related to the specific field is considered a priority for all stakeholders.

Application of double-pulse laser-induced breakdown spectroscopy (DP-LIBS), Fourier transform infrared micro-spectroscopy and Raman microscopy for the characterization of copper-sulfides.

The combined application of the structure sensitive techniques Fourier transform infrared μ-spectroscopy and Raman microscopy in conjunction with different approaches of laser-induced breakdown spectroscopy (LIBS) including the two-color double pulse (DP-LIBS) have been applied towards the characterization of whole ore copper-sulfide minerals. Discrete information from the surface of the whole ore minerals that lead to the establishment of infrared marker bands and from the surface of bioleached samples that allow the monitoring of jarosite and biofilm formation are provided by FTIR mapping experiments. Raman data can provide information related to the type of the mineral and of the secondary minerals formed on the surface of the ore.

Systems Approaches in the Common Metabolomics in Acute Lymphoblastic Leukemia and Rhabdomyosarcoma Cells: A Computational Approach.

Acute lymphoblastic leukemia is the most common childhood malignancy. Rhabdomyosarcoma, on the other hand, is a rare type of malignancy which belongs to the primitive neuroectodermal family of tumors. The aim of the present study was to use computational methods in order to examine the similarities and differences of the two different tumors using two cell lines as a model, the T-cell acute lymphoblastic leukemia CCRF-CEM and rhabdomyosarcoma TE-671, and, in particular, similarities of the metabolic pathways utilized by two different cell types in vitro.

A Neanderthal OAS1 isoform protects individuals of European ancestry against COVID-19 susceptibility and severity.

To identify circulating proteins influencing Coronavirus Disease 2019 (COVID-19) susceptibility and severity, we undertook a two-sample Mendelian randomization (MR) study, rapidly scanning hundreds of circulating proteins while reducing bias due to reverse causation and confounding. In up to 14,134 cases and 1.2 million controls, we found that an s.

Photoreduction of carotenoids in the aerobic anoxygenic photoheterotrophs probed by real time Raman spectroscopy.

The Aerobic anoxygenic phototrophic bacteria (AAPB) Roseobacter denitrificans and Roseobacter litoralis are widespread in the bacterioplankton community with a particular role in the marine carbon cycle. Measurements of carotenoids isolated from dark-grown cells indicated the presence of spheroidenone (SO, N = 11) and of 3,4 dihydrospheroidenone (N = 10) in the carotenoids isolated from illuminated cells. Time-dependent Raman 514 nm excitation experiments of R.

Photosensitivity responses of probed by FTIR, fluorescence and Raman microspectroscopy.

Raman, fluorescence and FTIR experiments of prestine and -metal ion complexes grown in light and in dark were performed to probe the photosensitivity response of the cellular components in the marine bacterium. In the presence of Cu(ii) and Zn(ii) the frequency shifts of PO , C-O-C and C-O-P vibrations indicate metal binding to nucleic acids, carbohydrates and polysaccharides. We assign the observed bands in the 514.

Extracellular electron uptake from carbon-based π electron surface-donors: oxidation of graphite sheets by probed by Raman and FTIR spectroscopies.

In this work we report Raman and FTIR evidence for extracellular electron uptake by from the solid phase carbon-based π-electron donor surface of graphite sheets. The primary step in the reaction is the intercalation of water on the surface of graphite followed by the formation of EPS and proceeds to form graphite oxide (GO) with a Raman / = 0.3 ratio which represents the highest defect content in the carbon lattice reported by bio-oxidation process.

Multi-electron reduction of Wells-Dawson polyoxometalate films onto metallic, semiconducting and dielectric substrates.

The investigation of conditions allowing multi-electron reduction and reoxidation of polyoxometalate (POM) films onto solid substrates is considered an issue of critical importance for their successful incorporation in electronic devices, different types of sensors and catalytic systems. In the present paper, the rich multi-electron redox chemistry of films of Wells-Dawson ammonium salts, namely (NH4)6P2Mo18O62 and (NH4)6P2W18O62, on top of metallic (Al), semiconducting (ITO) and dielectric (SiO2) substrates under ambient conditions is investigated. The respective Keggin heteropolyacids, H3PMo12O40 and H3PW12O40, are also investigated for comparison.

Intervention to decrease emergency department crowding: does it have an effect on return visits and hospital readmissions?

Study Objectives: We evaluate the effect of a multifaceted intervention to decrease emergency department crowding on the incidence of return visits to the ED or a hospital ward. The intervention included increased emergency physician coverage, the designation of physician coordinators, and new hospital policies regarding laboratory, consultation, and admission procedures.

Methods: The incidence of return visits within 7 days of discharge was estimated in samples from 2 populations (ie, patients discharged from the ED and patients discharged from the hospital) and during a 12-month period before and a 12-month period after the implementation of the intervention.

Efficacy of inhaled steroids (beclomethasone dipropionate) for treatment of mild to moderately severe asthma in the emergency department: a randomized clinical trial.

Study Objective: To examine the efficacy of an inhaled steroid, when added to a standard regimen of beta-agonist therapy, in the treatment of patients with mild to moderately severe asthma in the emergency department.

Methods: A convenience sample of adult patients with asthma (FEV1 % predicted 40% to 69%) presenting to the ED was randomly assigned in a double-blind fashion into 2 treatment groups. The first group received 2.

Emergency department use and misuse.

We conducted a 14-day survey of the emergency department (ED) at a university tertiary care teaching hospital to examine appropriate and inappropriate use of the ED. The results are based on a convenience sample of 849 patients, selected to represent a 1-week period. Three categories (CAT) of patients were defined.