The features of the growth of Azotobacter vinelandii IMV V-7076 and Bacillus subtilis IMV V-7023 in optimized environments in single and mixed culture at deep cultivation to simulate the accumulation stage (capacity) biomass biotechnology creation of complex bacterial preparation for crop production. When growing Bacillus subtilis IMV B-7023 in monoculture specific growth rate of the bacteria was higher than that of Azotobacter vinelandii IMV B-7076. Therefore, by cultivating a mixed culture medium in the fermenter was inoculated with the bacterial suspension A.
View Article and Find Full Text PDFNutrient medium for cultivation of Bacillus subtilis IMV V-7023 was optimized by the method of the orthogonal Latin rectangles. Optimum concentrations were investigated in the medium of carbon source (15.0 g/l of molasses), nitrogen (2.
View Article and Find Full Text PDFWe studied the effect of a clay mineral, palygorskite, on the physiological activity of Azotobacter chroococcum and the phosphate-mobilizing bacterium Bacillus subtilis, as well as their mixed cultures, under various oxygen supply conditions during the utilization of phosphorus from readily and poorly soluble compounds (K2HPO4 x 3H2O) and (Ca3(PO4)2), respectively. During cultivation of the bacteria in a nutrient medium with Ca3(PO4)2, the number of microorganisms was higher than that observed in a medium with K2HPO4. An increase in oxygen mass transfer in the nutrient medium was followed by a rise in the number of Bacillus subtilis cells and an inhibition of Azotobacter chroococcum growth.
View Article and Find Full Text PDFThe experience in surgical treatment of 326 patients with IV-VI degree chronic lower limb venous insufficiency (CEAP) is analyzed. Correction of disorders of venous hemodynamics was performed with surgical methods and sclerotherapy. The best clinical and functional results in long-term period were achieved in patients undergone surgery in an early stage of the disease without deep veins valves insufficiency and external trophic disorders.
View Article and Find Full Text PDFKhirurgiia (Mosk)
September 2005
Specific CT-symptoms are reliable criteria for differential diagnosis of benign lesions of the liver. The method permits to diagnose exactly cystic lesions of the liver in 95.3% cases, and tumor lesions -- in 83%.
View Article and Find Full Text PDFThe disturbance of redox and acid-base state in the tissues of rats with experimental rickets and in clinics has been studied. It was shown that administration of namacit for the directed correction of the disturbance of homeostasis allows decreasing the doses of vitamin D.
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