Publications by authors named "Tsai Ping Li"
The kringle 1 domain of human hepatocyte growth factor (HGFK1) was previously shown to inhibit bovine aortic endothelial cell proliferation, suggesting that it might be an antiangiogenic molecule. Here, we evaluated the in vivo efficacy of a recombinant adenoassociated virus carrying HGFK1 (rAAV-HGFK1) for the treatment of hepatocellular carcinoma (HCC) in a rat orthotopic HCC model and explored its molecular mechanisms in vitro in both endothelial and tumor cells. We first showed that rAAV-HGFK1 treatment significantly prolonged the survival time of rats transplanted with tumor cells.
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- The study aimed to discover new tumor markers and target genes in liver cancer by analyzing differentially expressed genes using cDNA microarray technology.
- The researchers synthesized labeled cDNAs from both cancerous and adjacent non-cancerous liver tissues, leading to an analysis of 8,400 genes, from which they confirmed differential expressions of selected genes through additional methods like Northern blot and RT-PCR.
- They identified four significant genes associated with hepatocarcinoma, with three genes (Beclin 1, RbAp48, Pir51) showing increased expression and one gene (aldolase b) showing decreased expression in tumor tissues.
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- The study aimed to identify point mutations in the LPTS gene, a candidate tumor suppressor and telomerase inhibitor, in liver cancer.
- Using various assays, researchers discovered five mutations in tumor cell lines, with specific alterations noted in exons 2, 5, and 7, but the most common mutation did not significantly affect telomerase activity.
- The findings suggest that LPTS mutations are quite rare in hepatocellular carcinoma and imply that other factors, like epigenetics, may contribute more significantly to the gene's inactivation.
Aim: p73, as a novel member of a family of p53-related transcription factors, shares redundant functions with p53, such as the abilities of inducing apoptosis and suppressing growth. It is well known that p53 can repress HBV expression and transcription efficiently. The aim of this paper is to investigate the transcriptional effect of p73alpha and p73beta on hepatitis B virus (HBV) and to understand the correlation between HBV and p73.
View Article and Find Full Text PDFHigh-level expression and secretion of recombinant mouse endostatin by Escherichia coli.
Protein Expr Purif
April 2002
The expression of murine endostatin was achieved by placing its gene downstream of an alkaline phosphatase gene (phoA) promoter. To ensure proper folding and secretion of the recombinant protein, the mouse endostatin was fused with alkaline phosphatase signal peptide. SDS/polyacrylamide gel electrophoresis analysis of the culture medium of recombinant Escherichia coli cells revealed that endostatin was efficiently secreted.
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