Influence of Vibrio anguillarum culture conditions on the efficacy of bacterin-based vaccines in lumpfish (Cyclopterus lumpus).

Lumpfish (Cyclopterus lumpus) is used as cleaner fish to control sea lice infestations in Atlantic salmon (Salmo salar) farms across the North Atlantic. Vibrio anguillarum, the causative agent of vibriosis, is a recurrent bacterial pathogen affecting lumpfish. Bacterin-based vaccines are frequently used to control vibriosis in finfish, but their efficacy is not always consistent.

Role of cold shock proteins B and D in subsp. physiology and virulence in lumpfish ().

Cold shock proteins (Csp) are pivotal nucleic acid binding proteins known for their crucial roles in the physiology and virulence of various bacterial pathogens affecting plant, insect, and mammalian hosts. However, their significance in bacterial pathogens of teleost fish remains unexplored. subsp.

Inactivated Aeromonas salmonicida impairs adaptive immunity in lumpfish (Cyclopterus lumpus).

Aeromonas salmonicida, a widely distributed aquatic pathogen causing furunculosis in fish, exhibits varied virulence, posing challenges in infectious disease and immunity studies, notably in vaccine efficacy assessment. Lumpfish (Cyclopterus lumpus) has become a valuable model for marine pathogenesis studies. This study evaluated several antigen preparations against A.

Transcriptome profiling of lumpfish (Cyclopterus lumpus) head kidney to Renibacterium salmoninarum at early and chronic infection stages.

Renibacterium salmoninarum causes Bacterial Kidney Disease (BKD) in several fish species. Atlantic lumpfish, a cleaner fish, is susceptible to R. salmoninarum.

Haemato-Immunological Response of Immunized Atlantic Salmon () to Challenge and Antigens.

Winter ulcer disease is a health issue in the Atlantic salmonid aquaculture industry, mainly caused by . Although vaccination is one of the effective ways to prevent bacterial outbreaks in the salmon farming industry, ulcer disease related to bacterial infections is being reported on Canada's Atlantic coast. Here, we studied the immune response of farmed immunized Atlantic salmon to bath and intraperitoneal (ip) challenges and evaluated the immunogenicity of cell components.

Comparative Genomic Analysis of Virulent () Serotypes Revealed Genetic Diversity and Genomic Signatures in the O-Antigen Biosynthesis Gene Cluster.

is the most frequent pathogen affecting fish worldwide. The only known virulent strains of are serotypes O1, O2, and O3. Genetic differences between the serotypes that could shed insight on the evolution and serotype differences of this marine pathogen are unknown.

Role of riboflavin biosynthesis gene duplication and transporter in virulence in marine teleost fish.

Active flavins derived from riboflavin (vitamin B) are essential for life. Bacteria biosynthesize riboflavin or scavenge it through uptake systems, and both mechanisms may be present. Because of riboflavin's critical importance, the redundancy of riboflavin biosynthetic pathway (RBP) genes might be present.

Multi-Organ Transcriptome Response of Lumpfish () to Subspecies Systemic Infection.

Lumpfish is utilized as a cleaner fish to biocontrol sealice infestations in Atlantic salmon farms. , a Gram-negative facultative intracellular pathogen, is the causative agent of furunculosis in several fish species, including lumpfish. In this study, lumpfish were intraperitoneally injected with different doses of to calculate the LD.

CD45 in ocular tissues during larval and juvenile stages and early stages of V. anguillarum infection in young lumpfish (Cyclopterus lumpus).

Immune responses to infectious diseases impacting lumpfish (Cyclopterus lumpus) eye tissue are only starting to be studied at a molecular and histopathological level. In this study, we extend our understanding of lumpfish sensory organ anatomy, of components of the lumpfish nasal and ocular immune system and the nature of the intraocular response to Vibrio anguillarum infection. We have evaluated the expression of cluster of differentiation (CD) 45 protein, a tyrosine phosphatase, in larval and juvenile lumpfish tissues in order to spatially survey ocular and related head structures that may participate in early stages of intraocular immune responses.

Comparative Reverse Vaccinology of , , , and , Frequent Pathogens of Atlantic Salmon and Lumpfish Aquaculture.

Marine finfish aquaculture is affected by diverse infectious diseases, and they commonly occur as co-infection. Some of the most frequent and prevalent Gram-negative bacterial pathogens of the finfish aquaculture include , , , and . To prevent co-infections in aquaculture, polyvalent or universal vaccines would be ideal.

Lumpfish () Is Susceptible to Infection and Induces Cell-Mediated Immunity in the Chronic Stage.

is a Gram-positive, intracellular pathogen that causes Bacterial Kidney Disease (BKD) in several fish species in freshwater and seawater. Lumpfish () is utilized as a cleaner fish to biocontrol sea lice infestation in Atlantic salmon () farms. Atlantic salmon is susceptible to , and it can transfer the infection to other fish species.

Oral Immunization of Larvae and Juvenile of Lumpfish () against Does Not Influence Systemic Immunity.

, a marine bacterial pathogen that causes vibriosis, is a recurrent pathogen of lumpfish Lumpfish is utilized as a cleaner fish in the Atlantic salmon () aquaculture in the North Atlantic region because of its ability to visualize and prey on the ectoparasite sea lice () on the skin of Atlantic salmon, and its performance in cold environments. Lumpfish immunity is critical for optimal performance and sea lice removal. Oral vaccine delivery at a young age is the desired method for fish immunization because is easy to use, reduces fish stress during immunization, and can be applied on a large scale while the fish are at a young age.

A Novel Marine Pathogen Isolated from Wild Cunners (): Comparative Genomics and Transcriptome Profiling of sp. Strain J380.

Cunner () is a cleaner fish being considered for utilized in the North Atlantic salmon () aquaculture industry to biocontrol sea lice infestations. However, bacterial diseases due to natural infections in wild cunners have yet to be described. This study reports the isolation of sp.

Using crime script analysis to understand wildlife poaching in Vietnam.

Poaching can contribute to the failure of biodiversity conservation efforts and inflict diverse harms on human livelihoods. We applied crime script analysis to the case of snare poaching-an illegal hunting activity-in three Vietnamese protected areas. Our goal was to enhance the understanding about the opportunity structure underlying snare poaching to advance the suite of community-based crime prevention activities.

CD10 Cells and IgM in Pathogen Response in Lumpfish () Eye Tissues.

Lumpfish (), a North Atlantic "cleaner" fish, is utilized to biocontrol salmon louse () in Atlantic salmon () farms. Lumpfish require excellent vision to scan for and eat louse on salmon skin. The lumpfish eye immune response to infectious diseases has not been explored.

Comparative Genomics Analysis of Isolated from Lumpfish () in Newfoundland Reveal Novel Chromosomal Organizations.

is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish (), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice () infestations in the Atlantic salmon () aquaculture industry. is one of the most frequent bacterial pathogens affecting lumpfish.

Aeromonas salmonicida infection kinetics and protective immune response to vaccination in sablefish (Anoplopoma fimbria).

Effective vaccine programs against Aeromonas salmonicida have been identified as a high priority area for the sablefish (Anoplopoma fimbria) aquaculture. In this study, we established an A. salmonicida infection model in sablefish to evaluate the efficacy of commercial vaccines and an autogenous vaccine preparation.

Vibrogen-2 vaccine trial in lumpfish (Cyclopterus lumpus) against Vibrio anguillarum.

Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to biocontrol sea lice infestations in Atlantic salmon aquaculture. However, bacterial infections are affecting cleaner fish performance. Vibrio anguillarum, the aetiological agent of vibriosis, is one of the most frequent bacterial infections in lumpfish, and effective vaccine programmes against this pathogen have been identified as a high priority for lumpfish.

Antibiotic Discovery with Synthetic Fermentation: Library Assembly, Phenotypic Screening, and Mechanism of Action of β-Peptides Targeting Penicillin-Binding Proteins.

In analogy to biosynthetic pathways leading to bioactive natural products, synthetic fermentation generates mixtures of molecules from simple building blocks under aqueous, biocompatible conditions, allowing the resulting cultures to be directly screened for biological activity. In this work, a novel β-peptide antibiotic was successfully identified using the synthetic fermentation platform. Phenotypic screening was carried out in an initially random fashion, allowing simple identification of active cultures.

Trade and conservation implications of new beak and feather disease virus detection in native and introduced parrots.

Psittacine beak and feather disease (PBFD), caused by Beak and feather disease virus (BFDV), has spread rapidly around the world, raising concerns for threatened species conservation and biosecurity associated with the global pet bird trade. The virus has been reported in several wild parrot populations, but data are lacking for many taxa and geographical areas with high parrot endemism. We aimed to advance understanding of BFDV distribution in many data-deficient areas and determine phylogenetic and biogeographic associations of the virus in 5 parrot species across Africa, the Indian Ocean islands, Asia, and Europe and focused specifically on the highly traded and invasive Psittacula krameri.

A new species of (Anura: Megophryidae) from Vietnam.

We describe a new, medium-sized Leptolalax species from north central Vietnam. Leptolalax puhoatensis sp. nov.

Interview-based sighting histories can inform regional conservation prioritization for highly threatened cryptic species.

The use of robust ecological data to make evidence-based management decisions is frequently prevented by limited data quantity or quality, and local ecological knowledge (LEK) is increasingly seen as an important source of information for conservation. However, there has been little assessment of LEK's usefulness for informing prioritization and management of landscapes for threatened species, or assessing comparative species status across landscapes.A large-scale interview survey in the Annamite Mountains (Vietnam and Lao PDR) compiled the first systematic LEK data set for saola , one of the world's rarest mammals, and eight other ungulates.