Oxidative stress response in SH-SY5Y cells exposed to short-term 1800 MHz radiofrequency radiation.

The exact mechanism that could explain the effects of radiofrequency (RF) radiation exposure at non-thermal level is still unknown. Increasing evidence suggests a possible involvement of reactive oxygen species (ROS) and development of oxidative stress. To test the proposed hypothesis, human neuroblastoma cells (SH-SY5Y) were exposed to 1800 MHz short-term RF exposure for 10, 30 and 60 minutes.

In vitro non-thermal oxidative stress response after 1800 MHz radiofrequency radiation.

In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field strength was 30 V/m and specific absorption rate (SAR) was calculated to be 1.

Cell oxidation-reduction imbalance after modulated radiofrequency radiation.

Aim of this study was to evaluate an influence of modulated radiofrequency field (RF) of 1800 MHz, strength of 30 V/m on oxidation-reduction processes within the cell. The assigned RF field was generated within Gigahertz Transversal Electromagnetic Mode cell equipped by signal generator, modulator, and amplifier. Cell line V79, was irradiated for 10, 30, and 60 min, specific absorption rate was calculated to be 1.

Histological and cytological examination of rat reproductive tissue after short-time intermittent radiofrequency exposure.

The unfavourable outcomes of mobile phone use on male fertility have still not been fully elaborated. To establish the potentially adverse effects of everyday exposure to radiofrequency radiation (RF) on humans, we performed a controlled animal study that aimed to investigate the influence of RF radiation on rat testis histology as well as the amount, mobility, and structure of epididymal free sperm cell population. Eighteen adult male rats were divided into two groups of nine.

Biological indicators in response to radiofrequency/microwave exposure.

Over the years, due to rapid technological progress, radiation from man-made sources exceeded that of natural origin. There is a general concern regarding a growing number of appliances that use radiofrequency/ microwave (RF/MW) radiation with particular emphasis on mobile communication systems. Since nonthermal biological effects and mechanisms of RF/MW radiation are still uncertain, laboratory studies on animal models, tissues, cells, and cell free system are of extraordinary importance in bioelectromagnetic research.

Induced sputum evaluation in restorers and conservators of cultural heritage.

The objective of this study was to examine induced sputum (IS) cells profile from restorers/conservators of cultural heritage (restorers) with no lower airway symptoms and normal ventilatory lung function. The study involved 22 restorers and 48 control workers. Medical interview, physical examination, spirometry, skin prick testing to inhalatory allergens, and IS collection were performed.

The effects of bluephase LED light on fibroblasts.

Objective: The aim of this study was to evaluate the effect of Bluephase light emitting diode (LED) light on cell viability, colony-forming ability and proliferation in V79 cell culture and to determine how much the temperature of the nutrient medium rose.

Methods: The investigation included a low (L), soft start (S) and high (H) illumination mode for 20, 40 and 80 seconds. The viability was determined by the trypan blue exclusion test, colony-forming ability by counting colonies 7 days after exposure and cell proliferation by the cell counts on 5 post-exposure days.

Non-thermal biomarkers of exposure to radiofrequency/microwave radiation.

This article gives a review or several hypotheses on the biological effects of non-thermal radiofrequency/microwave (RF/MW) radiation and discusses our own findings from animal and in vitro studies performed over the last decade. We have found that RF/MW radiation disturbs cell proliferation and leads to cell differentiation in the bone marrow, which is reflected in the peripheral blood of rats. Repeated RF/MW radiation can also temporarily disrupt melatonin turnover.

Effect of electromagnetic radiofrequency radiation on the rats' brain, liver and kidney cells measured by comet assay.

The goal of study was to evaluate DNA damage in rat's renal, liver and brain cells after in vivo exposure to radiofrequency/microwave (Rf/Mw) radiation of cellular phone frequencies range. To determine DNA damage, a single cell gel electrophoresis/comet assay was used. Wistar rats (male, 12 week old, approximate body weight 350 g) (N = 9) were exposed to the carrier frequency of 915 MHz with Global System Mobile signal modulation (GSM), power density of 2.

[Issues related to long-term asbestos use and manufacture].

Extensive measures to ban mining, manufacture, use, and trade of asbestos and asbestos materials have been taken worldwide. In this century asbestos will continue to be an economic, industrial, health, social, and environmental issue. Five thousand products that are still in use have been inherited from a century of asbestos processing.

Assessment of cyto/genotoxicity of irinotecan in v79 cells using the comet, micronucleus, and chromosome aberration assay.

Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 microg mL-1), recommended monotherapy dose (9 microg mL-1), and recommended combined therapy dose (4.5 microg mL-1) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test.

Exposure to poultry dust and health effects in poultry workers: impact of mould and mite allergens.

Purpose: The aim of the study was to evaluate exposure to moulds and house dust mite Dermatophagoides pteronyssinus in poultry farms, and related health effects in poultry workers (PW).

Methods: The study involved 41 PW and 45 control office workers. Working environment was evaluated for D.

Evaluation of basal DNA damage and oxidative stress in Wistar rat leukocytes after exposure to microwave radiation.

The aim of this study was to assess whether microwave-induced DNA damage is basal or it is also generated through reactive oxygen species (ROS) formation. After having irradiated Wistar rats with 915MHz microwave radiation, we assessed different DNA alterations in peripheral leukocytes using standard and formamidopyrimidine DNA-glycosylase (Fpg)-modified comet assay. The first is a sensitive tool for detecting primary DNA damage, and the second is much more specific for detecting oxidative damage.

Disturbance of cell proliferation in response to mobile phone frequency radiation.

The aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2+/-0.

Respiratory function in pesticide workers.

Objective: Pesticide aerosols are frequently toxic irritants associated with respiratory symptoms and lung function impairment.

Methods: A cross-sectional study examined the prevalence of acute and chronic respiratory symptoms and lung function abnormalities in 82 workers employed in processing pesticides and in 60 control workers not exposed to irritants and employed in a soft drink bottling plant.

Results: The prevalence of almost all chronic respiratory symptoms was greater among pesticide workers than among controls.

Dose- and mode-dependent effect of halogen dental curing blue light on the V79-cell line.

The aim of this study was to evaluate the time and dose dependent effect of halogen light from dental curing unit on the cell viability, colony-forming ability and proliferation of the V79 cell culture. The investigation included the medium mode (M), exponential (E) and standard (S) illumination mode for 20, 40 and 80 seconds. The viability was determined using the trypan blue exclusion test.

In vitro testing of cellular response to ultra high frequency electromagnetic field radiation.

The aim of this study was to evaluate whether low-level, ultra high frequency (UHF) irradiation of 935 MHz influences the cell structure and growth of V79 cells. UHF field was generated inside a Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell) with a Hewlett-Packard signal generator. The electric field strength was 8.

Impact of 864 MHz or 935 MHz radiofrequency microwave radiation on the basic growth parameters of V79 cell line.

The aim of this study was to evaluate and compare the influence of 864 MHz and 935 MHz radiofrequency/microwave (RF/MW) fields on the growth, colony-forming ability, and viability of V79 cells (continuous line). Cell samples with 1 x 10(4) V79 cells each, were exposed to continuous wave frequencies of 864 MHz and 935 MHz for 1, 2 and 3 hours. Exposed samples were matched with unexposed control samples.

Comparison of 864 MHz and 935 MHz microwave radiation effects on cell culture.

The objective of this study was to compare the effects of 864 MHz and 935 MHz radiofrequency/microwave radiation on the ability of V79 cells to proliferate, form colonies and on their viability. For one, two and three hours, the cells were exposed to the 864 MHz field in a transversal electromagnetic mode cell (TEM) connected with amplifier and to the 935 MHz field in a gigahertz transversal electromagnetic mode cell (GTEM) equipped with a signal generator. The average specific absorption rate (SAR) was 0.

Erythropoietic dynamic equilibrium in rats maintained after microwave irradiation.

The aim of study was to define influence of radiofrequency microwave (RF/MW) radiation on erythropoiesis in rats. The kinetics of polychromatic erythrocytes (PCEs) and micronucleated (MN) PCEs in the bone marrow (BM) and peripheral blood (PB) of rats during the intermittent subchronic experiment was followed. Rats were exposed 2h/day, 7 days/week to RF/MW of 2.

Erythropoietic changes in rats after 2.45 GJz nonthermal irradiation.

The purpose of this study was to observe the erythropoietic changes in rats subchronically exposed to radiofrequency microwave (RF/MW) irradiation at nonthermal level. Adult male Wistar rats (N=40) were exposed to 2.45 GHz continuous RF/MW fields for 2 hours daily, 7 days a week, at 5-10 mW/cm2.

Blood-forming system in rats after whole-body microwave exposure; reference to the lymphocytes.

The influence of 2.45 GHz microwave (RF/MW) irradiation on blood-forming cells after whole-body irradiation of rats was investigated. The exposures were conducted with a field power density of 5-10 mW/cm2, and whole-body average specific absorption rate (SAR) of 1-2 W/kg.

Investigation of the genotoxic effect of microwave irradiation in rat bone marrow cells: in vivo exposure.

An in vivo mammalian cytogenetic test (the erythrocyte micronucleus assay) was used to investigate the extent of genetic damage in bone marrow red cells of rats exposed to radiofrequency/microwave (RF/MW) radiation. Wistar rats (n = 40) were exposed to a 2.45 GHz continuous RF/MW field for 2 h daily, 7 days a week, at a power density of 5-10 mW/cm(2).