Canine testicular tumors: An 11-year retrospective study of 358 cases in Moscow Region, Russia.

Background And Aim: Canine testicular tumors are among the most common reproductive tract tumors in male dogs and have been studied in many countries. However, to the best of our knowledge, studies with a large sample size have not been conducted in Russia. This study aimed to provide the latest information on the prevalence of canine testicular tumors in the Veterinary Oncology Scientific Center for Small Animals "Biocontrol" in Moscow, Russia, in 2010-2020 and the characteristics of the affected canine population.

[EFFECT OF LONGIDAZE ON MICROBIAL LANDSCAPE OF CERVICAL CANAL AND UTERINE CAVITY DURING THERAPY OF WOMEN WITH CHRONIC NONSPECIFIC ENDOMETRITIS AND UTERUS MYOMA].

Aim: Evaluation of microbiological effectiveness of longidaze preparation use during therapy of active non-specific endometritis in women with uterus myoma.

Materials And Methods: 2 groups of women were formed by a method of random selection. The first was composed of patients with active chronic endometritis, that had received standard etiotropic antibacterial therapy.

[Salicylic and jasmonic acids in regulation of the proantioxidant state in wheat leaves infected by Septoria nodorum Berk].

Influence of mediators of the signal systems of salicylic (SA) and jasmonic (JA) acids and their mixture on reactive oxygen species' (ROS) (superoxide radical O2*- and H2O2) generation and activity of oxidoreductases (oxalate oxidase, peroxidase and catalase) in leaves of wheat Triticum aestivum L. infected by Septoria leaf blotch pathogen Septoria nodorum Berk. has been studied.

[Influence of Ca2+ ions on metabolism of active oxygen species in combined cultures of wheat calluses with the fungus Tilletia caries].

The effect of Ca2+ on morphophysiological parameters of calluses of wheat Triticum aestovum L., the level of active oxygen species, and the activity of oxalate oxidase, peroxidase, and catalase is investigated in the case of infestation with the fungus Triticum aestivum causing ball smut. The concentration of O2-, H2O2, and activity of oxidoreductases (oxalate oxidase, peroxidase, and catalase) depends on the content of Ca2+ ions in the culture medium of calluses.

[Angiotensin-converting enzyme stability in the presence of zinc-ions different concentrations].

Stability of angiotensin-converting enzyme was studied as a dependence on the zinc-ions concentrations brining in the apo-enzyme. Our data were discussed in the terms of a set of initial permissible conformation conditions of a protein (conformation distribution). Apo-enzyme was shown to be able to the radiation activation that is disappearing in the presence of even 10(-6 )mol/l of the zinc-ions.

[Effect of hydrogen peroxide on morphological characteristics and resistance of wheat calluses to the stinking smut fungus].

We studied the effect of hydrogen peroxide on morphological characteristics and resistance of common wheat calluses (Triticum aestivum L.) to Tilletia caries Till. The induction of the defense response and morphogenesis in calluses depended on H2O2 concentration.

[Salicylic acid induces resistance to Septoria nodorum Berk. in wheat].

The effect of salicylic acid (SA) on oxalate oxidase and peroxidase activities and hydrogen peroxide (H2O2) production in leaf cells has been studied in wheat of the susceptible cultivar Zhnitsa infected by the fungus Septoria nodorum, a pathogen of wheat leaf blotch. The results show that fungal hyphae spread into interstices between mesophyll cells and that infected tissues contain H2O2. Treatment with SA results in enhanced H2O2 production in mesophyll cells, which is due to activation of oxalate oxidase and peroxidase in the cell wall.

[Plant resistance inductors and active forms of oxygen. III. The influence of bisol 2 and baitan on morphogenesis and defense response of wheat non-morphogenic callus cells infected with smut agent].

A study was made of the influence of bisol 2 and baitan compounds on morphogenesis and defence response of wheat calluse cells infected with bunt agent in associated with oxalate oxidase activation. After introduction of bisol and baitan into cultivation medium, dense area with meristema zones, germs of shoots and rhizoids appeared on non-morphogenic calluses, which correlated with enzyme activation. Parenchyma-like cells, generating hydrogen peroxide, were seen in the site of pathogen penetration under infestation, but were never revealed in control.

[The effect of pathogens and phytohormones on the rate of oxidation of phenols by oxalate oxidase in wheat seedlings].

We studied the effect of various fungal phytopathogens and exogenous phytohormones on the rate of o-phenylenediamine oxidation mediated by oxalate oxidase, which is one of protective proteins of wheat. The pattern of changes in o-phenylenediamine oxidation rate depended on pathogen type, cultivar resistance, and phytohormone class. This reaction is proposed as a model reaction for studying plant response to infection and effects of various physiologically active compounds.

[Plant resistance inductors and active forms of oxygen. II. The influence of chitooligosaccharides on the production of hydrogen peroxide with the involvement of oxalate oxidase in common cultures of wheat calluses and bunt pathogen].

We have studied the influence of various concentrations of chitooligosaccharides (CO) on hydrogen peroxide (H2O2) generation, with the involvement of oxalate oxidase (OO), in rhizoids and in zones of hard brand pathogen penetration, as well as on oxalate oxidase activity in wheat calluses. In the control group, diaminobenzidin (DAB)-staining was typical of 30% of peripheral rhizoid cells, which provided their resistance during infection. In the zone of fungus penetration, the appearance of DAB-stained parenchyma-like cells was observed.

[Plant resistance inductors and active forms of oxygen. I. The influence of salicylic acid on hydrogen peroxide production in common cultures of wheat callus and bunt pathogen].

The influence of salicylic acid (SA) on cell resistance to bunt pathogen in wheat calluses has been studied. Cell staining by diaminobenzidin substratum (DAB-cells) was conditioned by hydrogen peroxide (H2O2) generation with the involvement of oxalate oxidase (OO). In the control group, DAB-staining was typical only of rhizoid cells (up to 50%).

[Radioenzymatic investigation of membrane and soluble forms of angiotensin-converting enzyme in acetate-phosphate buffer].

The dose response of soluble and membrane forms of angiotensin-converting enzyme to gamma-irradiation is investigated at different pH values of the medium and at different concentrations of acetate-phosphate buffer. Membrane form of the enzyme is more stable shows principally other conformational equilibrium than the soluble form. "Splitted" activation peaks on the curves of the enzyme dose response are observed.

A hydrophobic site on the surface of the angiotensin-converting enzyme molecule.

Using the hydrophobic fluorescent dye 8-anilino-1-naphthalenesulfonic acid (8-ANS), a hydrophobic site on the surface of the protein globule of angiotensin-converting enzyme (ACE) from bovine lung was found. The dissociation constant of the ACE-8-ANS complex was estimated as 1.5 +/- 0.

Chitooligosaccharide-induced activation of o-phenylenediamine oxidation by wheat seedlings in the presence of oxalic acid.

A method for determination of oxidation of phenolic compounds by intact wheat seedlings using o-phenylenediamine (OPD) was developed. The reaction is initiated by the addition of oxalic acid to the incubation medium. It is suggested that an endogenous peroxidase and hydrogen peroxide formed during oxidation of oxalic acid by endogenous oxalate oxidase are involved in OPD oxidation.

[Development of blunt Tilletia caries (D.C.) Tul. pathogen on embryogenic wheat callus].

The development of Tilletia caries on embryogenic and morphogenic wheat calluses from germination of spores up to sorus formation have been investigated. Pathogene growth was similar in variants with wheat calluses of susceptible species Triticum aestivum and resistant species T. timopheevii, but intensity of its development of embryogenic calluses of both species was lower, than on morphogenic.

[Development of Tilletia caries (D.C.) Tul. in callus and suspension wheat cultures].

All phases of ontogenesis of Tilletia caries were found in combined suspension and callus culture of the wheat and causative agent of common bunt of wheat. Newly formed spores were observed on calluses of the susceptible species Triticum aestivum and resistant species T. timopheevi within 90 and 120 days after inoculation, respectively, which can be used for their production in the laboratory.

[Radiation activation of angiotensin-converting enzyme during low-dose irradiation].

Radiation activation of angiotensin-converting enzyme (respect to Cbz-Phe-His-Leu as substrate) was obtained at the gamma (137Cs, t(irr) = 10s-2h, D approximately 3 Gy)- and X (plasma foces source, t(irr) = 10(-9)s, Cu-filter, D approximately 2 x 10(-5) Gy)-irradiation. The inactivation of the horseradish peroxidase at the same X-irradiation dose (2 x 10(-5) Gy) took place. Based on the experimental data and on the mathematical model proposed by us we made a conclusion that the special points exist on the dose response curves.