Intradural T12-L1 disc herniation in a patient with achondroplasia: A case report.

Background: There are very few reports of intradural disc herniations associated with achondroplasia described in the literature.

Case Description: A patient with achondroplasia presented with progressive paraparesis attributed to a magnetic resonance-documented intradural disc herniation at the T12-L1 level occupying more than 90% of the spinal canal. It was successfully removed through a T12 laminectomy with durotomy; note a laminectomy would have been contraindicated if this had been an extradural anterior/anterolateral disc.

Transformative tales: The role of story videos on children's reasoning about transgender identities.

The current study explored whether positive contact through stories could influence how young children think about transgender identities and gender in general. A total of 174 children ages 5-6 and 9-10 were randomly assigned to one of three conditions: Jazz (participants watched a video regarding a transgender child named Jazz), Blue (participants watched a video regarding a marker that looked red on the outside but inside was really blue) and control (no video). Both videos described the main character as feeling different inside than outside, and their social transition to their preferred identity; researcher scaffolding supported the video messages.

Adolescents' exposure to community violence and associations with after-school activities across two samples.

Introduction: Exposure to community violence (ECV) continues to be a major public health problem among urban adolescents in the United States. We sought to identify subgroups of adolescents' ECV and examine how after-school activities are related to exposure subgroups across two samples.

Methods: In Study 1 there were 1432 adolescents (Cohort 9 n = 717, M = 11, and Cohort 12 n = 715, M = 14; 52% boys) from the Project on Human Development in Chicago Neighborhoods (1994-2002).

Women Versus Females: Gender Essentialism in Everyday Language.

How do different words referring to gender/sex categories reflect and/or shape our understanding of gender/sex concepts? The current study examined this issue by assessing how individuals use gender/sex terms (females, males, women, men). Participants recruited through MTurk (N = 299) completed an online survey, rating the terms on nine dimensions, completing a fill-in-the-blank task, and reporting gender essentialist beliefs. Overall, participants rated the words females/males as more biological and technical, and women/men as higher on all other dimensions (e.

Total Hip Arthroplasty Digital Templating: Size Predicting Ability and Interobserver Variability.

Background: During the last century, total hip arthroplasties have become more popular. They have had a huge impact on the quality of life, pain, range of motion, social interaction, and psychological well-being. A number of studies have emphasized the importance of using templates to choose the appropriate implant size when planning the surgery.

Risk factors for local recurrence of fibromatosis.

Objective: To evaluate the clinical, radiological and histological factors that can predict local recurrence of fibromatosis.

Methods: A retrospective study was conducted on 51 patients diagnosed with fibromatosis in this hospital from 1983 to 2014. The mean follow-up was 83 months.

Clinical and radiological short-term complications after single-stage bilateral uncemented total hip arthroplasty.

Purpose: Economic crisis time gives to efficient procedures an important role in healthy system. Total hip replacement is a common bilateral orthopedic procedure, but there exists an important controversy to perform it in single or two stages. Our aim is to report our clinical and radiological short-term complications of bilateral uncemented total hip arthroplasty in a single time.

Differential expression levels of aroma-related genes during ripening of apricot (Prunus armeniaca L.).

Fruit aroma is a complex trait, particularly in terms of the number of different biosynthetic pathways involved, the complexity of the final metabolites, and their regulation. In order to understand the underlying biochemical processes involved in apricot aroma, four cDNAs (Pa-aat, EU784138; Pa-adhEU395433; Pa-pdcEU395434; and Pa-loxEU439430) encoding an alcohol acyl transferase (AAT), alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC), and lipoxygenase (LOX), respectively, were isolated and characterized at four stages of maturity in Prunus armeniaca L. cv.

Differential distribution of the vasopressin V receptor along the rat nephron during renal ontogeny and maturation.

Background: Ontogeny and cellular distribution of vasopressin receptors in the kidney are key factors determining the role of vasopressin in renal physiology. Expression of vasopressin V(2) receptor (V(2)R) mRNA and the immunoreactive protein in rat kidney were investigated.

Methods: An antiserum directed to epitope TLD25 of the rat V(2)R sequence was characterized by Western blotting.

Dispersive mixing in a batch electrophoretic cell with Eyring fluids.

The main objective of this study is analysis of dispersive mixing inside a batch electrophoretic cell due to Joule heating, especially for the case of non-Newtonian carriers. To this end, a carrier fluid that follows the Eyring rheological model is used in the analysis of the species convective-diffusive equation that describes the solute motion inside the device. The hydrodynamic problem (Bosse, M.

Batch electrophoretic cells with Eyring fluids: analysis of the hydrodynamics.

Mixing and dispersion phenomena caused by the carrier fluid in an electrophoretic cell is the main subject of this study. In particular, the effects of Joule heating on temperature and velocity profiles for Eyring-model fluids (EMF) are studied. The heat transfer is sequentially coupled with momentum transfer to derive an analytical expression for both the temperature and the velocity profiles.

Low-dose inhaled carbon monoxide reduces pulmonary vascular resistance during acute hypoxemia in adult sheep.

Carbon monoxide (CO) is produced by the action of the heme oxygenase (HO) complex through the oxidation of heme. CO, like nitric oxide (NO), is a molecular gas that among other actions stimulates guanylyl cyclase and increases cGMP levels in smooth muscle cells, regulating the vascular tone. Acute hypoxia generates pulmonary hypertension and increases the expression of inducible HO isoform (HO-1) in the vascular endothelium.

Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.

Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Phosphorylation was mediated largely by V(1)receptor subtype since it was inhibited by selective V(1)antagonist and was only partially elicited by the V(2)agonist, desmopressin.

Immunolocalization of V1 vasopressin receptors in the rat kidney using anti-receptor antibodies.

By using immunocytochemical techniques we have been able to localize the V1 vasopressin receptor in the rat kidney. Immunoblotting using an antiserum raised against an affinity-purified vasopressin receptor showed a 55,000 daltons protein band that has a molecular mass similar to that of the liver V1 vasopressin receptor, as demonstrated by cross-linking studies. Immunoblotting of the antibody showed a band of 55,000 daltons in A-10 cells, which contains the V1 subtype, whereas it did not stain LLC-PK1 cells, which possess the V2 subtype, showing that the antibody recognizes the V1 vasopressin receptor.

[Nitric oxide in respiratory diseases].

Recent reports have described a pathogenic role of nitric oxide in several respiratory disease. It is specially useful in the adult respiratory distress syndrome, where it acts as a selective vasodilator and improves gas exchange, decreasing pulmonary shunting. Although it has a proven bronchodilator effect, its therapeutic role in diseases such as asthma and chronic limitation of airway flow is not well defined.

Renal function and cardiopulmonary bypass: effect of perfusion pressure.

Controversy continues as to whether hypotension during cardiopulmonary bypass (CPB) impairs intraoperative and postoperative renal function. Therefore, 21 patients with normal renal function (plasma creatinine less than 1.2 mg/dL, creatinine clearance greater than 70 mL/min), aged 50 to 70 years, without associated pathology, scheduled for elective coronary surgery were studied prospectively.

Modulation of the affinity of the vasopressin receptor by magnesium ions.

The binding of [3H]vasopressin (AVP) and the 125I-labelled vasopressin antagonist (VP-AT) d(CH2)5[Tyr2(Me),Tyr9(NH2)]AVP to rat liver membranes was examined with or without the addition of milimolar concentrations of divalent cations. The binding of vasopressin was enhanced by Mg2+ and Co2+ and markedly decreased by EGTA. The addition of EGTA and Mg2+ together restored the binding to a value similar to that of Mg2+ alone.

Dopaminergic regulation of luteinizing hormone-releasing hormone release at the median eminence level: immunocytochemical and physiological evidence in hens.

Theoretically, the most effective inhibitory control of hypophysiotropic luteinizing hormone-releasing hormone (LHRH) release might occur through a presynaptic inhibition of LHRH neuronal terminals at the median eminence (ME) level. Since: (a) we have recently reported the existence of synaptic contacts between dopamine- and LHRH-containing processes in the ewe ME, and (b) nutritional deprivation induces an ovulatory failure in both birds and mammals, we have assessed the possibility that the anovulatory state induced by feed withdrawal (FW) in laying hens, might be caused by a dopaminergic inhibition of LHRH release at the ME level. Laying hens at the start (35 weeks old) and end (75 weeks old) of their commercial egg-laying life were killed at 0, 1, 2 and 4 days after FW.

Identification of the V1 vasopressin receptor by chemical cross-linking and ligand affinity blotting.

Chemical and photoaffinity cross-linking experiments as well as ligand affinity blotting techniques were used to label the V1 vasopressin receptor. In order to determine the optimal reaction conditions, pig liver membranes were incubated with 5 nM [8-lysine]vasopressin (LVP) labeled with 125I and then cross-linked with the use of DMS (dimethyl suberimidate), EGS [ethylene glycol bis(succinimidyl succinate)] or HSAB (hydroxysuccinimidyl p-azidobenzoate) at different final concentrations. Consistently, EGS was found to label with high yield one band of Mr 60,000 in rat and pig liver membranes when used at a final concentration between 0.

Identification and distribution of the cell types in the pituitary gland of Austromenidia laticlavia (Teleostei, Atherinidae).

By using antisera against human pituitary hormones in immunocytochemistry in combination with classical cytochemical techniques, we have been able to identify the different cell types in the adenohypophysis of the Austromenidia laticlavia and to determine their location. Antisera against prolactin and growth hormones did not stain cells in the pituitary of Austromenidia, whereas antisera against beta-endorphin, LH, and beta-TSH selectively cross-reacted with cells which have a specific location within the adenohypophysis. The beta-endorphin antiserum stained the periodic acid-Schiff (PAS)-negative cells in the pars intermedia and also, though faintly, the PAS-negative cells in the internal border of the rostral pars distalis (RPD).

Sex-hormone-binding globulin in the plasma of normal men and patients with benign prostatic hypertrophy.

Sex-hormone-binding globulin (SHBG) was measured in the plasma of normal men aged 30--50 yr, normal men 50--70 yr of age, and men 50--70 yr of age suffering from benign prostatic hypertrophy (BPH). The level of binding proteins in normal "young" men decreased approximately 45% in normal "older" men. A 100% rise was observed in BPH patients as compared to normal older men.