[The synchronization of cells in culture by macromolecular complexes of platinum].

Divalent platinum compounds: cis-dichlorodiammineplatinum (II) (DDP) and its new macromolecular complexes poly (hexacis[chloroammineaquaplatinum(II)])-micro- deoxyribonucleats (MCP) have been used for the first time as the cell culture synchronization inductors. It is established that when monolayer cell cultures are incubated in presence of the above compounds, the conditions are created under which cell cycle is blocked at the S-phase and more then 80% of cells are accumulated in the Gl-phase. MCP compound is preferable in this case.

[Preclinical trials of polyplatillen, the antitumor activity of a complex platinum compound with DNA on a leukemia P388 model in a HEp-2 cell culture (1)].

The authors studied the antitumour activity of a new complex platinum compound (DDP-DNA) in mice with leukemia P-388 and in cell culture. DDP-DNA was introduced intraperitoneally 24 hours after inoculation of 2 x 10(6) tumour cells. It was established that by its antitumour activity DDP-DNA was not inferior then DDP.

[Use of polyplatillen in the treatment of stage IV malignant neoplasms].

Results of the use of polyplatillen--a preparation consisting of sodium nucleinate (deoxyribonucleic acid) and cisplatin combined in the saline balanced solution by means of the original technique--in cancer of the ovaries (198 patients), liver (62), pancreas (35), lungs (12), stomach (5) are presented. Clearly pronounced specific activity of the preparation developed is noted.

[Preclinical study of the general toxic effect of polyplatillen].

Toxic action of polyplatillen, a principally new platinum compound, was studied on laboratory animals and cell culture. When compared with cis-dichlorodiamminplatinum, the preparation is 10-fold less toxic in animals and 100-fold--in the cellular systems. Reversibility of damaging action of polyplatillen at "non-toxic" dosages conditions the perspectiveness of its further clinical trials.

[Effect of exogenous neoplasm DNA on the course of Swetz leukemia in non-inbred rats of different age groups].

Experiments on 150 non-inbred white rats have shown that preliminary administration of high polymer native DNA isolated from Swetz leukemia tumor suppresses the tumor growth. Tumor DNA combined with BCG vaccine in young animals suppresses and in old ones stimulates the tumor growth. These results correlate with DNA and CIC content in the blood serum.

[Combination methods of local treatment of tumors of the abdominal organs].

On the basis of the analysis of the results of treatment by the methods of cryosurgery, roentgeno-endovascular embolization, local use of immobilized chemo-preparations and systemic administration of a high-molecular platinum compound of 218 patients with IV stage tumours of different location, it is shown that the mean lifetime of the patients increases 12-fold, the number of postoperative complications and lethality decrease.

[Methylation of DNA from rat lymphoid tissues in development of immune response].

Within 4 days after immunization of rats by means of sheep erythrocyte suspension (of various concentration) analysis of nucleotide composition of DNA, isolated from the animal lymphoid tissue, was carried out using cellulose thin-layer chromatography. The immunization was shown to affect distinctly the DNA methylation in rat lymphoid tissue; sometimes, it altered the DNA nucleotide composition. The data obtained correspond with the well-recognized phenomenon on dependence of the immune response on the antigen concentration.