Light-induced H generation in a photosystem I-O-tolerant [FeFe] hydrogenase nanoconstruct.

The fusion of hydrogenases and photosynthetic reaction centers (RCs) has proven to be a promising strategy for the production of sustainable biofuels. Type I (iron-sulfur-containing) RCs, acting as photosensitizers, are capable of promoting electrons to a redox state that can be exploited by hydrogenases for the reduction of protons to dihydrogen (H). While both [FeFe] and [NiFe] hydrogenases have been used successfully, they tend to be limited due to either O sensitivity, binding specificity, or H production rates.