Evidence for protein dielectric relaxations in reaction centers associated with the primary charge separation detected from Rhodospirillum rubrum chromatophores by combined photovoltage and absorption measurements in the 1-15 ns time range.

Fast photovoltage measurements in Rhodospirillum rubrum chromatophores in the nanosecond time range, escorted by time-resolved absorption measurements, are described. Under reducing conditions, the photovoltage decayed significantly faster than the spectroscopically detected charge recombination of the radical pair P(+)H(A)(-). This indicates the occurrence of considerable dielectric relaxations.

Escape probability and trapping mechanism in purple bacteria: revisited.

Despite intensive research for decades, the trapping mechanism in the core complex of purple bacteria is still under discussion. In this article, it is attempted to derive a conceptionally simple model that is consistent with all basic experimental observations and that allows definite conclusions on the trapping mechanism. Some experimental data reported in the literature are conflicting or incomplete.

Uphill energy transfer in LH2-containing purple bacteria at room temperature.

Uphill energy transfer in the LH2-containing purple bacteria Rhodopseudomonas acidophila, Rhodopseudomonas palustris, Rhodobacter sphaeroides, Chromatium vinosum and Chromatium purpuratum was studied by stationary fluorescence spectroscopy at room temperature upon selective excitation of the B800 pigments of LH2 and the B880 pigments of LH1 at 803 nm and 900 nm, respectively. The resulting fluorescence spectra differed significantly at wavelengths shorter than the fluorescence maximum but agreed at longer wavelengths. The absorption spectra of the species studied were decomposed into five bands at approx.

Spectroscopic and molecular characterization of a long wavelength absorbing antenna of Ostreobium sp.

One of the strains of the marine green alga Ostreobium sp. possesses an exceptionally large number of long wavelength absorbing chlorophylls (P. Haldall, Biol.

Theories for kinetics and yields of fluorescence and photochemistry: how, if at all, can different models of antenna organization be distinguished experimentally?

The models most commonly used to describe the antenna organization of the photosynthetic membrane are the connected units model and the domain model. The theoretical descriptions of the exciton dynamics according to these models are reviewed with emphasis on a common nomenclature. Based on this nomenclature we compare for the two models the kinetics and yields of photochemistry and fluorescence under non-annihilation and annihilation conditions both under continuous light and under flash excitation.

The cyanobacterium Spirulina platensis contains a long wavelength-absorbing pigment C738 (F76077K) at room temperature.

Spirulina platensis is a cyanobacterium which usually lives under high-light conditions. Nonetheless, it is thought to contain the most red-shifted antenna pigment of all known Chl a-containing phototrophic organisms, as shown by its 77 K fluorescence peaking at 760 nm. To exclude preparation artifacts and to exclude the possibility that long wavelength-absorbing pigments form only when the temperature is lowered to 77 K, we carried out experiments with whole cells at room temperature.

Competition between annihilation and trapping leads to strongly reduced yields of photochemistry under ps-flash excitation.

Excitation of photosynthetic systems with short intense flashes is known to lead to exciton-exciton annihilation processes. Here we quantify the effect of competition between annihilation and trapping for Photosystem II, Photosystem I (thylakoids from peas and membranes from the cyanobacterium Synechocystis sp.), as well as for the purple bacterium Rhodospirillum rubrum.

Antenna organization in purple bacteria investigated by means of fluorescence induction curves.

Fluorescence induction curves of purple bacteria (Rs. rubrum, Rps. viridis and Rb.

Theory of fluorescence induction in photosystem II: derivation of analytical expressions in a model including exciton-radical-pair equilibrium and restricted energy transfer between photosynthetic units.

The theoretical relationships between the fluorescence and photochemical yields of PS II and the fraction of open reaction centers are examined in a general model endowed with the following features: i) a homogeneous, infinite PS II domain; ii) exciton-radical-pair equilibrium; and iii) different rates of exciton transfer between core and peripheral antenna beds. Simple analytical relations are derived for the yields and their time courses in induction experiments. The introduction of the exciton-radical-pair equilibrium, for both the open and closed states of the trap, is shown to be equivalent to an irreversible trapping scheme with modified parameters.

Primary charge separation in photosystem I: a two-step electrogenic charge separation connected with P700+A0- and P700+A1- formation.

A PS I membrane preparation from a PS II deficient mutant of Synechocystis sp. PCC 6803 (psb DI/DII/C) was investigated by picosecond photovoltage and fluorescence measurements. The photovoltage kinetics show two distinct phases.

Why do thylakoid membranes from higher plants form grana stacks?

Chloroplasts contain a system of membrane sacs, the thylakoids, some of which are stacked to form grana (singular, granum), whereas others float freely in the stroma. It is on the thylakoid membranes that the electron carriers necessary for photosynthesis reside. There has been continuous speculation and discussion about the function of the grana ever since Menke postulated their lamellar nature in 1939.

Why does the light-gradient photovoltage from photosynthetic organelles show a wavelength-dependent polarity?

The light-gradient photovoltage from photosynthetic organisms and organelles is thought to arise from the primary charge separation in the reaction centers. The current explanation of the effect is the stronger excitation of the membrane side of a vesicle facing the light source than the one on the opposite side. Together with the known orientation of reaction centers, this explanation predicts unequivocally the polarity of the photovoltage.

Theoretical fluorescence induction curves derived from coupled differential equations describing the primary photochemistry of photosystem II by an exciton-radical pair equilibrium.

Fluorescence induction curves were calculated from a molecular model for the primary photophysical and photochemical processes of photosystem II that includes reversible exciton trapping by open (PHQ(A)) and closed (PHQ(-) (A)) reaction centers (RCs), charge stabilization as well as quenching by oxidized (P(+)HQ((-)) (A)) RCs. For the limiting case of perfectly connected photosynthetic units ("lake model") and thermal equilibrium between the primary radical pair (P(+)H(-)) and the excited singlet state, the primary reactions can be mathematically formulated by a set of coupled ordinary differential equations (ODE). These were numerically solved for weak flashes in a recursive way to simulate experiments with continuous illumination.

Long-wavelength absorbing antenna pigments and heterogeneous absorption bands concentrate excitons and increase absorption cross section.

The light-harvesting apparatus of photosynthetic organisms is highly optimized with respect to efficient collection of excitation energy from photons of different wavelengths and with respect to a high quantum yield of the primary photochemistry. In many cases the primary donor is not an energetic trap as it absorbs hypsochromically compared to the most red-shifted antenna pigment present (long-wavelength antenna). The possible reasons for this as well as for the spectral heterogeneity which is generally found in antenna systems is examined on a theoretical basis using the approach of thermal equilibration of the excitation energy.

Photoelectric study on the kinetics of trapping and charge stabilization in oriented PS II membranes.

Excitation energy trapping and charge separation in Photosystem II were studied by kinetic analysis of the fast photovoltage detected in membrane fragments from peas with picosecond excitation. With the primary quinone acceptor oxidized the photovoltage displayed a biphasic rise with apparent time constants of 100-300 ps and 550±50 ps. The first phase was dependent on the excitation energy whereas the second phase was not.

Excitation trapping and primary charge stabilization in Rhodopseudomonas viridis cells, measured electrically with picosecond resolution.

The transmembrane primary charge separation in the photosynthetic bacterium Rhodopseudomonas viridis was monitored by electric measurements of the light-gradient type [Trissl, H. W. & Kunze, U.

Spatial correlation between primary redox components in reaction centers of Rhodopseudomonas sphaeroides measured by two electrical methods in the nanosecond range.

Relative distances between the the primary donor P, the intermediary pheophytin acceptor H, and the iron-quinone acceptor Q of bacterial reaction centers were determined by recording laser flash-induced photovoltages in two experimental systems with nanosecond time resolution. In one system a suspension of chromatophores was subjected to a light gradient and in the other system chromatophores were spread at a heptane/water interface. The 10-ns back reaction occurring in reaction centers with reduced Q could be time resolved.

On the rise time of the R1-component of the "early receptor potential": evidence for a fast light-induced charge separation in rhodopsin.

The rising phase of the R1-component of the early receptor potential from isolated cattle retinas was measured with high time resolution. When the measuring capacitance was 133 pF, a latency of about 200 ns was observed. A rise time of about 0.

The concept of chemical capacitance, A critique.

The concept of chemical capacitance as introduced by Hong and Mauzerall (Proc. Natl. Acad.

II. Electrical measurements in the nanosecond range of the charge separation from chloroplasts spread at a heptane-water interface. Application of a novel capacitive electrode.

Spinach chloroplasts are spread at a heptane-water interface. Applying a novel capacitative electrode introduced in the preceding paper (Trissl, H.-W.

I. Novel capacitative electrode with a wide frequency range for measurements of flash-induced changes of interface potential at the oil-water interface. Mechanical construction and electrical characteristics of the electrode.

The mechanical construction and the electrical properties of a new type of capacitative electrode for the oil-water interface are described. The electrode is designed to detect changes of the interface potential induced by photochemical, photophysical, and photobiological reactions occurring at the interface. The construction is based on capacitative coupling of two aqueous compartments separated by a thin Teflon film.