Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal.

Objectives: This study was carried out to determine the prevalence of metallo-β-lactamases (MBLs) producing Pseudomonas aeruginosa in imipenem-nonsusceptible isolates and to detect MBL-encoding genes among MBLs-positive isolates.

Results: Metallo-β-lactamases production was detected in 68.6% isolates of P.

Co-existence of and genes of isolated from Nepal: antimicrobial resistance and clinical significance.

Background: Molecular analysis of carbapenem-resistant genes in , an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of

Methods: were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and genes. The antibiotic susceptibility testing was performed using disc diffusion and E-test method.