Publications by authors named "Tripathi G"

Saprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater cages in Indian reservoirs and to determine minimum inhibitory concentrations of different antifungal compounds against the fungal hyphae and zoospores. The fungal isolates grown on potato dextrose agar showed an abundance of gemmae, elongated mycelia, non-septate hyphae, primary zoospores, mature zoosporangia with numerous zoospores, cysts with bundles of long hairs and were further identified as Saprolegnia parasitica following PCR amplification and sequencing of internal transcribed spacer region.

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Nanotechnology offers a transformative approach to augment plant growth and crop productivity under abiotic and biotic stress conditions. Nanomaterials interact with key phytohormones, triggering the synthesis of stress-associated metabolites, activating antioxidant defense mechanisms, and modulating gene expression networks that regulate diverse physiological, biochemical, and molecular processes within plant systems. This review critically examines the impact of nanoparticles on both conventional and genetically modified crops, focusing on their role in nutrient delivery systems and the modulation of plant cellular machinery.

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Background And Aims: Acute liver failure (ALF) has high mortality predominantly due to compromised immune system and increase vulnerability to bacterial and fungal infections.

Method: Plasma lipidome and fungal peptide-based-community (mycobiome) analysis were performed in Discovery cohort (40-ALF, 5-healthy) and validated in a validation cohort of 230-ALF using High-resolution-mass-spectrometry, artificial-neural-network (ANN) and machine-learning (ML).

Results: Untargeted lipidomics identified 2,013 lipids across 8 lipid-groups.

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Type 2 diabetes mellitus (T2DM) and progressive liver disease are 2 of the most significant global health concerns, and they have alarming and ever-increasing prevalence. A growing body of literature has demonstrated a potential multilateral link between gut microbiome dysbiosis and the development and progression of the above-mentioned conditions. Modulation of gut microbial composition from the norm is due to changes in diet allied with external factors such as age, genetics, and environmental changes.

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The continuous evolution of SARS-CoV-2 has led to the emergence of several variants of concern (VOCs) that significantly affect global health. This study aims to investigate how these VOCs affect host cells at proteome level to better understand the mechanisms of disease. To achieve this, we first analyzed the (phospho)proteome changes of host cells infected with Alpha, Beta, Delta, and Omicron BA.

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Introduction: Maxillofacial injuries are usually associated with ophthalmic injuries that may be mild to severe, and may even result in loss of integrity of the orbital skeleton and impairment of the visual apparatus.

Aim: To evaluate the pattern of ophthalmic injuries associated with maxillofacial fractures in patients who reported to a tertiary care hospital, associated with a medical college in M.P, India.

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Article Synopsis
  • Carcinoma of the gall bladder (CAGB) has a low survival rate, but analyzing bile samples can help identify molecular indicators for early detection of the disease.
  • A study on 87 bile samples found specific proteomic and metabolomic signatures associated with CAGB that correlated with increased inflammation and altered energy pathways compared to healthy controls.
  • The top four metabolites showed over 99% accuracy and sensitivity for CAGB detection in further validation tests, suggesting that bile analysis could lead to improved early diagnosis methods.
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Objective: The choice of a stable reference gene plays a crucial role in gene expression analysis. In most cases, the choice of housekeeping gene (HKG) is quite random. However, the expression of HKGs varies under different experimental conditions and in different tissues.

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Article Synopsis
  • - Industrialization and globalization have heightened the demand for petroleum products, leading to greater fossil fuel use and greenhouse gas emissions, which calls for sustainable alternatives.
  • - Algae are emerging as a promising source for biofuels, regarded as third-generation biofuels due to their high biomass production and multiple derivable fuels, like bio-hydrogen and bio-ethanol, using various processing methods.
  • - Despite challenges like high cultivation costs, the potential of algae-derived biofuels can be enhanced through the use of byproducts, paving the way for more economical and environmentally sustainable energy solutions.
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  • - The study focused on the haemoglobin protein structure in Labeo catla, highlighting the encoding of its subunits (α and β) by HBA and HBB genes, which were sequenced to get their complete coding sequences.
  • - Phylogenetic analysis indicated that Labeo catla is closely related to other cyprinid fish, particularly Labeo rohita, and bioinformatics tools were used to predict gene function and protein structure.
  • - Under hypoxic conditions, both HBA and HBB genes were found to be significantly upregulated, leading to increased haematological indices; however, a subsequent decrease in their expression resulted in higher mortality rates among the fish.
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This protocol describes a robust method for the extraction of intra and extracellular metabolites of gut bacterial mono and co-cultures. In recent years, the co-culture techniques employed in the field of microbiology have demonstrated significant importance in regard to understanding cell-cell interactions, cross-feeding, and the metabolic interactions between different bacteria, fungi, and microbial consortia which enable the mimicking of complex co-habitant conditions. This protocol highlights a robust reproducible physiologically relevant culture and extraction protocol for the co-culture of gut bacterium.

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The present study aimed to isolate a bioflocculating fungal strain from wastewater collected from a local bike garage. The isolate showed maximum similarity to species The fungus was identified as species (accession no OP703382). The isolated fungus was evaluated in terms of biomass recovery efficiency in Sp.

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Aim: To compare the impact of submucosal dexamethasone (4 mg) administered after the onset of local anesthesia on postoperative discomfort after third molar surgery and compare the parameters with a control group that did not receive the drug.

Methods: A total of 60 patients indicated for surgical removal of impacted mandibular third molars (mesioangular, Class II or III, and position B or C) were randomly divided into two groups of 30 patients each. After the onset of local anesthesia, the first group (Group A) received a submucosal injection of 4 mg dexamethasone adjacent to the surgical site, and the control group (Group B) received no drug.

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Wild food plants (WFPs) grow naturally and are essential components of the diet and conventional food chain. These plants are underutilized despite being nutritious and highly bio-active compounds. Wild food consumption has declined over the last generation owing to life-style changes and lower availability, and it is still consumed by marginalized communities.

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Developed here is a highly fluorescent organic N,O bidentate BF complex from isoxazole in the presence of a Ru(II) catalyst. Herein, the complexation proceeds a selective N-O cleavage of the isoxazole ring. The complex shows absorption () in the range of 352-363 nm with an extinction coefficient () in the range of 8000-64 000 M cm, and fluorescence emission () in the range of 413-485 nm with a Stokes shift of 61-125 nm having quantum yield up to 33%.

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Intervertebral Disc Degeneration is a pathophysiological condition that primarily affects the spinal discs, causing back pain and neurological deficits. It is caused by the contribution of several factors such as genetic predisposition, age-related degeneration, and lifestyle choices like obesity and physical activity. Even though there are medications to treat pain, there is a lack of medicines for a complete cure.

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Milk is a good source of nutrition but is also a source of allergenic proteins such as α-lactalbumin, β-lactoglobulin (BLG), casein, and immunoglobulins. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas technology has the potential to edit any gene, including milk allergens. Previously, CRISPR/Cas has been successfully employed in dairy cows and goats, but buffaloes remain unexplored for any milk trait.

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Background: Patients with pediatric cirrhosis-sepsis (PC-S) attain early mortality. Plasma bacterial composition, the cognate metabolites, and their contribution to the deterioration of patients with PC-S to early mortality are unknown. We aimed to delineate the plasma metaproteome-metabolome landscape and identify molecular indicators capable of segregating patients with PC-S predisposed to early mortality in plasma, and we further validated the selected metabolite panel in paired 1-drop blood samples using untargeted metaproteomics-metabolomics by UHPLC-HRMS followed by validation using machine-learning algorithms.

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Article Synopsis
  • The study investigates how periphyton can reduce arsenic toxicity in Labeo rohita fish while considering food safety for humans.
  • The introduction of periphyton alongside arsenic-contaminated water showed significant improvements in various health parameters of the fish, including respiratory function and immune response.
  • The findings indicate that using periphyton in aquaculture can lead to safer fish with lower arsenic levels, thereby reducing potential health risks for consumers.
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No water body is resilient to afflicts of algal bloom, if goes unmanaged. With the increasing trend of intensification, eutrophication and climate change, Labeo rohita (rohu) is highly anticipated to suffer from the deleterious effects of bloom and eventually its toxins. A comprehensive study was conducted to understand the toxicopathological effects of microcystin-LR (MC-LR) in rohu following intraperitoneal injection of 96 h-LD dose i.

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In severe acute malnutrition, micronutrient deficiency as well as protein energy malnutrition is a major obstacle to growth & development. Iron deficiency dominates the spectrum of nutritional anemia. After taking informed consent, 211 SAM children and 211 age-and sex-matched healthy children with normal nutritional status were enrolled for the study.

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Purpose of the study The current study had two goals: first, it compared the radiological and functional results of the ipsilateral shaft and proximal femur fractures treated by using two different methods, i.e., single implant vs dual implants.

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Article Synopsis
  • The study investigates the gut microbiome composition in advanced fingerling and fingerling stages of striped pangasius catfish and catla under both healthy and diseased conditions.
  • Healthy fish were injected with a bacterial pathogen (A. hydrophila) to analyze changes in their intestinal microbiota through 16s metagenomic analysis.
  • The findings reveal differences in dominant bacterial phyla between the life stages of fish, highlighting the potential for identifying bacteria that could enhance fish health and inform disease prevention strategies in aquaculture.
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Recombination activating genes (RAGs) mediates the process of rearrangement and somatic recombination (V(D)J) to generate different antibody repertoire. Studies on the expression pattern of adaptive immune genes during ontogenic development are crucial for the formulation of fish immunization strategy. In the present study, Nile tilapia was taken to explore the relative expression profile of RAG genes during their developmental stages.

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