3D analysis of thermal and stress evolution during laser cladding of bioactive glass coatings.

Thermal and strain-stress transient fields during laser cladding of bioactive glass coatings on the Ti6Al4V alloy basement were numerically calculated and analysed. Conditions leading to micro-cracking susceptibility of the coating have been investigated using the finite element based modelling supported by experimental results of microscopic investigation of the sample coatings. Consecutive temperature and stress peaks are developed within the cladded material as a result of the laser beam moving along the complex trajectory, which can lead to micro-cracking.

Retroperitoneal, mediastinal, and subcutaneous emphysema, complicating colonoscopy and rectal polypectomy.

Complications of flexible endoscopy-though still rare-are increasing in frequency lately as more invasive procedures are routinely performed. Perforation, hemorrhage, coagulation disorders, thrombophlebitis, and splenic rupture have all been reported to complicate colonoscopy and colorectal polypectomies. In this paper, we report on a case of retroperitoneal, mediastinal, and neck surgical emphysema, complicating colonoscopy and rectal polypectomy, presented initially as a change in the voice and facial swelling.

Epidermoid splenic cyst presented as huge splenic abscess: a case report.

Epidermoid splenic cysts are very rare. Symptoms emerge because of enlargement, infection, haemorrhage or rupture. Although splenectomy is indicated for large cysts, minimally invasive and preservation procedures, such as partial splenectomy or total cystectomy with splenorrhaphy, have been increasingly used during the last decade.

Pachytene karyotype analysis of the nematode Meloidogyne spartinae in relation to the genus Meloidogyne.

Seven synaptonemal complexes (SC) were present in the pachytene nuclei of Meloidogyne spartinae. The SC was bipartite and consisted of two lateral elements, while lacking a striated central element. This pattern was similar in all Meloidogyne species studied thus far with the exception of M.

Hermaphroditism in Meloidogyne hapla.

Hermaphrodites were detected in diploid and polyploid isolates of population 86-Va of Meloidogyne hapla. Young hermaphrodites are indistinguishable from normal females. Initially, hermaphrodite ovaries are filled with oocytes at various stages of development.

Further studies on the role of polyploidy in the evolution of meloidogyne.

Two tetraploid isolates of Meloidogyne hapla, 86P and E289P, with haploid chromosome numbers of 34 and 28, respectively, were studied cytogenetically and biologically in relation to the diploid populations, 86-Va (n = 17) and E289-Taiwan (n = 14), from which they had been originally isolated. Both isolates were quite stable, converting to diploidy at the low rate of about 2.5%.

Demonstration of Multiple Mating in Heterodera glycines with Biochemical Markers.

Controlled crosses of Heterodera glycines were carried out by placing one o r more virgin females of known esterase phenotype on an agar plate and adding, at various time intervals, one or more males of different esterase phenotypes. Progeny (second-stage juveniles) of such crosses were propagated on soybeans, and 30 days later young females were subjected to electrophoretic analysis to determine their esterase phenotype. Esterase phenotypes that represented the heterozygous state of the maternal and paternal genomes confirmed the hybrid nature of the progeny and identified their male parent.

Isozyme phenotypes for the identification of meloidogyne species.

Extensive studies during the last 20 years have demonstrated that enzyme phenotypes, especially those of esterases, are species-specific for Meloidogyne and can be used as reliable taxonomic characters for identification of most major and several minor species of this genus. Recent progress in electrophoretic procedures and advanced computer technology have made available automated electrophoretic apparati that can process very thin polyacrylamide slab gels on which the phenotypes of two or more enzymes can be revealed from the protein extract of a single Meloidogyne female. Presently, such apparati facilitate objective species identification.

[Mucous retention cysts of the minor salivary glands. A specific type of mucocele].

The mucous retention cyst of the minor salivary glands represent a specific type of oral mucocele which is lined by epithelium. It is caused probably from partial or complete obstruction of a duct. It affects older patients (over 40 years of age) most commonly women and it is located in different sites than the ordinary mucocele.

DNA Complexity of the Root-knot Nematode (Meloidogyne spp.) Genome.

Cot curves derived from renaturation kinetics of sheared denatured DNA indicated that the genome of six populations representing the four most common root-knot nematode species (Meloidogyne incognita, M. arenaria, M. javanica, and M.

Genetic Analysis of Esterase Polymorphism in the Soybean Cyst Nematode, Heterodera glycines.

The genetic basis of esterase polymorphism in Heterodera glycines was investigated through controlled matings and analysis of F and F progeny. Three nematode lines, each fixed for a different esterase phenotype, were isolated and purified through repeated directional selection and inbreeding. Each phenotype was characterized by its distinct pair of closely spaced bands of esterase activity.

DNA Isolation and GC Base Composition of Four Root-knot Nematode (Meloidogyne spp.) Genomes.

Phenol extraction and cesium trifluoroacetate ultracentrifugation were compared for efficiency in the extraction of DNA from eggs and second-stage juveniles of four species of Meloidogyne. The second method proved to be more satisfactory in that it yielded larger amounts of DNA, shortened the extraction period, and reduced sample handling by eliminating phenol and ether extraction and RNAse treatment. It also made possible the extraction of DNA: from more than one sample at a time.

Enzymatic relationships and evolution in the genus meloidogyne (nematoda: tylenchida).

Thirty populations of Meloidogyne of diverse geographic origin representing 10 nominal species and various reproductive, cytological, and physiological forms known to exist in the genus were examined to determine their enzymatic relationships. The 184 bands resolved in the study of 27 enzymes were considered as independent characters. Pair-wise comparisons of populations were performed in all possible combinations to estimate the enzymatic distances (ED) and coefficients of similarity (S).

Cytogenetic Status of Meloidogyne (Hypsoperine) spartinae in Relation to Other Meloidogyne Species.

Four populations of Meloidogyne spartinae from the coast of North and South Carolina were identical cytogenetically. Fourteen rod-shaped chromosomes were present in oogonia and spermatogonia, whereas seven bivalents were observed in oocytes and spermatocytes. There were no distinguishable sex chromosomes.

Use of enzyme phenotypes for identification of meloidogyne species.

Enzyme phenotypes were obtained for 291 populations from 16 species of Meloidogyne originating from 65 countries. Soluble proteins from macerates of individual egg-laying females were separated by electrophoresis in 0.7-mm-thick polyacrylamide gels.

Gametogenesis and the Chromosomes of Meloidogyne nataliei: Not Typical of Other Root-knot Nematodes.

Studies of oogenesis and spermatogenesis revealed that Meloidogyne nataliei is a diploid, amphimictic species with four (n), relatively large chromosomes, and possibly with an XX female symbol-XY male symbol mechanism of sex determination. It differs considerably from all other amphimictic, or meiotically parthenogenetic, species of Meloidogyne which have 13-18 smaller chromosomes and from Meloidogyne (Hypsoperine) spartinae which has seven. Consequently, the taxonomic position of M.

Genetic Basis of the Epidemiologic effects of Resistance to Meloidogyne incognita in the Tomato Cultivar Small Fry.

The genetic nature of resistance and its epidemiologic effects on two Meloidogyne incognita populations were assessed in the F hybrid tomato cv. Small Fry. The progeny of a Small Fry x Small Fry cross segregated in a 3:1 resistant:susceptible ratio, indicating the presence of a single, completely dominant resistance gene (LMiR) in Small Fry.

The synaptonemal complexes of meloidogyne: relationship of structure and evolution of parthenogensis.

The synaptonemal complexes of three amphimictic (meiotic) strains of Meloidogyne are examined in this study. M. microtyla (n = 19) has a tripartite synaptonemal complex (SC) comprised of two lateral elements and one central region with a distinct central element.

Oogenesis and the Chromosomes of the Parthenogenic Root-knot Nematode Meloidogyne incognita.

220 populations of Meloidogyne incognita and related forms from 46 countries reproduced by mitotic parthenogenesis (apomixis). Determination of somatic chromosome numbers from oogonia and oocytes revealed the existence of a predominant, possibly triploid race A with 3n = 40 to 46 and a rare, diploid race B with 2n = 32 to 36 chromosomes. There is no correlation between cytological races and the four recognized host races of this species.

Pachytene karyotype analysis of tetraploid Meloidogyne hapla females by electron microscopy.

Pairing of homologous chromosomes results in the formation of 34 synaptonemal complexes (SC) at pachytene, corresponding to the 34 bivalents at metaphase I. No multivalent associations were observed and pairing occurs two-by-two. The modified SC, which lacks a central element, does not affect the pairing process.

The effect of methylprednisolone on the sodium and water content of the dog lung after hemorrhagic shock.

Out of 17 mongrel dogs, 3 were subjected on one and two hours of hemorrhagic shock, while the remaining four served as controls. In five of the thirteen dogs, 30 mg/kg of methylprednisolone sodium succinate were administered intravenously one hour after hemorrhage. All animals were sacrificed at the end of the experiment, their lungs were removed and the sodium and water content was measured.

Morphological comparison of meloidogyne female head structures, perineal patterns, and stylets.

The external morphology of female heads of three populations of each of two cytological races of Meloidogyne hapla (race A-meiotic, race B-mitotic) and single populations of M. arenaria, M. incognita, and M.

Karyotype analysis of the plant-parasitic nematode Heterodera glycines by electron microscopy. II. The tetraploid and an aneuploid hybrid.

In the plant-parasite nematode Heterodera glycines, 2 forms, in addition to the diploid (9 bivalents), have been isolated and analysed: a tetraploid (18 bivalents) and an aneuploid (14 bivalents, hybrid between the diploid and the tetraploid). Observations on the formation of their karyotypes indicates normal and non-homologous pairing. Eighteen normal synaptonemal complexes (SC) are present in pachytene nuclei of the tetraploid.