Automated cation-exchange chromatography of peptides at a high sensitivity. (0-0.1 Absorbance range).

A method is described where leaching of the column resin by pyridine-acetate buffers is minimal and excessive rise of the base-line of the chromatogram is avoided. The method can be used to fractionate peptide mixtures at a concentration of 2-20nmol/component.

Amino acid composition of human fibrinogen and anticoagulant derivatives.

1. The amino acid compositions of human fibrinogen and three intermediate anticoagulant derivatives were determined by column chromatography. The derivatives were isolated by ammonium sulphate fractionation and column electrophoresis from solutions of fibrinogen undergoing spontaneous breakdown.