[Kinetics of the antibacterial effect of ampicillin and sulbactam combinations in dynamic and static conditions].

Kinetics of antimicrobial effect (AME) of ampicillin/sulbactam combinations (ratios of 4:1 to 1:2) on ampicillin resistant bacterial strains producing beta-lactamases of types II, III, IV and V according to Richmond classification was studied with using the computerized system MS-2 (turbidimetric recording) and an in vitro dynamic model (microcalorimetric recording). The concentrations of the drugs in system MS-2 (static conditions) corresponded to the maximum ones observed in serum of humans after bolus intravenous administration of ampicillin in a dose of 0.5 g and sulbactam in doses of 0.

[Antibiotic therapy of angiogenic sepsis].

Introduction to medical practice of new penicillins, cephalosporins and aminoglycosides is one of the chief reserves for increasing efficacy of antibacterial therapy. The main schemes of antibiotic use in treatment of sepsis and individual regimens controlled by laboratory findings are discussed. Optimization of antibiotic therapy schemes is based on pharmacokinetic studies, quantitative assay of antibiotic sensitivity and determination of antibacterial activity of serum and other biosubstrates at definite periods after antibiotic administration.

[Comparative activity of new semisynthetic penicillins and their combinations with gentamycin against gram-negative bacteria].

Comparative antibacterial activity of two novel ureidopenicillins (azlocillin and piperacillin), carbenicillin and ampicillin against 170 clinical strains of Enterobacteriaceae and 43 strains of Pseudomonadaceae was studied. Higher antibacterial activity of azlocillin and piperacillin evident from lower frequency of resistant strains and lower MICs for the majority of the isolates was shown. Impact of the inoculum size on the MIC values was observed with respect to all the penicillins.

[Comparative evaluation of the antibacterial activity of aminoglycosides by means of microcalorimetry].

Possible use of microcalorimetry for comparative investigation of antibiotics is exemplified by estimation of the kinetics of the antibacterial effect of netilmicin, sisomycin and gentamycin sulfate on moderately sensitive strains of E. coli and P. aeruginosa.

[Pharmacokinetics of cefazolin during hemosorption and hemodialysis].

The influence of hemosorption and hemodialysis on the pharmacokinetics of cefazolin was studied in 20 patients with chronic and acute renal insufficiency. The integral mean value of the antibiotic extraction coefficient in hemosorption was approximately 2 times higher than that in hemodialysis. In the first case the value of this parameter systematically lowered with time (the constant of the process rate amounted to 0.

[Laboratory methods of controlling the effectiveness of antibacterial therapy].

The serum antibacterial activity (SAA) against causative agent isolated after the use of antibiotics was studied in 68 patients with pyoseptic diseases. The SAA ranged from 1:2 to 1:512 and depended on the antibiotic sensitivity of the causative agents. Antibiotic therapy was effective, when the SAA was equal to 1:8-1:512.

[Pharmacokinetics of sisomicin during hemosorption and hemodialysis].

The effect of hemosorption and hemodialysis on the pharmacokinetics of sisomicin was studied in 17 patients with acute and chronic renal insufficiency. The value of the antibiotic extraction coefficient in hemosorption was almost 2 times higher than that in hemodialysis. In patients on hemosorption, extracorporeal elimination of the antibiotic was of the saturation nature.

[Use of antibioticograms for individual programs of antibacterial therapy].

The increasing role of antibiotic-resistant microorganisms in etiology of bacterial infections and the increased number of the antibiotics used for the treatment of patients with purulent infections require a rational approach to the choice of the optimal drug. Antibiotic sensitivity of the causative agents of bacterial infections is considered at present as one of the main indices determining the efficacy of antibacterial therapy. Determination of antibiotic sensitivity by the disk diffusion method with quantitative and semi-quantitative interpretation of the results provides information for choosing the most effective drug and calculating its optimal dose in control therapy.

[Pharmacokinetic basis for using tobramycin and sisomicin in treating pyelonephritis of the transplanted kidney].

The pharmacokinetics of tobramycin and sisomicin in patients after kidney transplantations was studied. A significant variability of the pharmacokinetic parameters of tobramycin and sisomicin under conditions of the changing function of the kidney transplant was shown. This required individual control of the drug serum levels in such patients.

[Rapid microbiological method of determining the gentamicin, sisomicin and kanamycin concentrations in patients' serum].

A modification of the microbiological agar-diffusion method for rapid determination of gentamicin, sisomycin and kanamycin levels in the blood serum of patients is described. The decrease in the time for determination of the antibiotic levels in the serum specimens with the modified method was provided by the use of a higher inoculation dose of the test microbe, higher levels of the incubation temperature and an enriched nutrient medium. The assay time was decreased from 18 to 3--4 hours as compared to the routine agar-diffusion method.

[Comparative characteristics of the methods for assessing aminoglycoside extraction with the artificial kidney tobramycin clearance and dialyzability].

The pharmacokinetics of tobramycin after its intravenous or intramuscular injection in a dose of 80 mg for 60 minutes was studied in 8 patients with chronic glomerulonephritis in the terminal stage of chronic renal insufficiency. The drug levels wee determined in the arterial (CA) and venous (CV) blood and dialyzates (CD) during the hemodialysis (6 hours) and 13-70 hours before the hemodialysis. The antibiotic was administered simultaneously with connection of the "artificial kidney" apparatus (KIIL) or 1 hour after it.