1. Focal electrographic seizures arose in the CA1 region of rat hippocampal slices bathed in elevated (8.5 mM) external potassium [( K+]o).
View Article and Find Full Text PDFReduction of external calcium and magnesium from 1.5 to 1.2 mM intensified potassium-induced interictal bursts, increased the likelihood of electrographic seizure occurrence in CA1, and rendered seizure initiation independent of N-methyl-D-aspartate (NMDA) receptor activation.
View Article and Find Full Text PDFJ Neurophysiol
January 1988
1. The CA1 region of rat hippocampal slices bathed in 8.5 mM interstitial K+ ([K+]o) exhibited spontaneous 20- to 90-s electrographic seizures at regular intervals of 1-8 min.
View Article and Find Full Text PDFCrit Rev Neurobiol
January 1989
The inhibitory and excitatory amino acid neurotransmitter receptors in the mammalian central nervous system mediate functionally opposite synaptic responses yet appear to share certain structural features. Recent conceptual advances in this field have relied heavily on information obtained by single channel analyses, by the expression of receptors in oocytes, and by autoradiographic studies of receptor distribution among brain receptors. This article reviews the pharmacology, cellular physiology, and regional distribution of these receptors and discusses their role in several well-characterized neurological disease states.
View Article and Find Full Text PDFIncubation of 1321N1 human astrocytoma cells with carbachol resulted in a rapid loss of binding of [3H]N-methylscopolamine ([3H]NMS) to muscarinic cholinergic receptors measured at 4 degrees C on intact cells; loss of muscarinic receptors in lysates from the same cells measured with [3H]quinuclidinyl benzilate [( 3H]QNB) at 37 degrees C occurred at a slower rate. Upon removal of agonist from the medium, the lost [3H]NMS binding sites measured on intact cells recovered with a t1/2 of approximately 20 min, but only to the level to which [3H]QNB binding sites had been lost; no recovery of "lost" [3H]QNB binding sites occurred over the same period. Based on these data and the arguments of Galper et al.
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