Embryonic stem cells (ESCs) are essential for research on gene regulation and understanding differentiation processes, with NANOG being a key factor that keeps them in an undifferentiated state.*
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This study explores NANOG expression in mouse ESCs by cloning the bovine NANOG promoter and tagging it with a fluorescent marker, showing significant GFP expression during differentiation.*
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Findings indicate that the bovine NANOG 5'-flanking region behaves similarly to its human and mouse counterparts, suggesting potential insights for studying bovine ESCs using mouse models.*