Identification and functional analysis of a delta-6 desaturase from the marine microalga Glossomastix chrysoplasta.

A delta-6 (Delta6) desaturase gene was isolated from the marine microalga Glossomastix chrysoplasta, a stramenopile that produces large amounts of eicosapentaenoic acid (EPA). A functional analysis of this gene was performed in Saccharomyces cerevisiae. Isolation of the Delta6 fatty acid desaturase was achieved via reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed from conserved histidine motifs and 5' and 3' RACE.

Physiological studies of eicosapentaenoic acid production in the marine microalga Glossomastix chrysoplasta.

We describe the characterization of the microalga Glossomastix chrysoplasta, an eicosapentaenoic acid (EPA) producer in the Pinguiophyceae class, Chromophyte division. Growth conditions were selected to optimize algal growth and EPA production. EPA represented up to 30% of the fatty acid content of Glossomastix chrysoplasta, at levels of 22 mg EPA per gram dry weight.