Hydrolytic enzymes expressivity in different parts of the Rapana digestive system.

The relevance of comprehensive studies of the Rapana vital functions is determined by its considerab­le negative impact on the ecosystem of the Black Sea. The aim of the work was to find out the polymorphism and activity of the main hydrolases in the different parts of the digestive system of Rapana. Hydrolases (proteases, amylases, esterases, lipases and phosphatases) in glandular structures of the Rapana digestive system were studied by electrophoresis.

[Penetration of 35S-lipoate into cells of animals subjected to overloading with the lipoate].

Penetration of 35S-lipoate into nucleated and nucleus-free erythrocytes, isolated cells (enterocytes) and accumulating tissue preparations from intestinal mucosa was studied in presence or in absence of other vitamins. Effect on this process of overloading the penetration of 35S-lipoate into rat and pigeon erythrocytes was found to be increased; under these conditions the enterocytes bind less 35S-lipoate than in control animals. Water-soluble vitamins (nicotinate, riboflavine, thiamin and, especially, panthotenate) decreased the rate of 35S-lipoate penetration into the cells.