Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells.

Background: A novel model system to study the cellular, molecular, and genetic characteristics of breast cancer stem cells is needed. Personalized prognostic models are indispensable for assessing the effect of so-called tailor-made adjuvant therapy.

Materials And Methods: Surgically-extirpated tissues were dispersed with Dispase and cultured in commonly used medium.