Imaging of Oral SCC Cells by Raman Micro-Spectroscopy Technique.

We used Raman micro-spectroscopy technique to analyze the molecular changes associated with oral squamous cell carcinoma (SCC) cells in the form of frozen tissue. Previously, Raman micro-spectroscopy technique on human tissue was mainly based on spectral analysis, but we worked on imaging of molecular structure. In this study, we evaluated the distribution of four components at the cell level (about 10 μm) to describe the changes in protein and molecular structures of protein belonging to malignant tissue.

Cavitation inception pressure and bubble cloud formation due to the backscattering of high-intensity focused ultrasound from a laser-induced bubble.

Cavitation bubble cloud formation due to the backscattering of high-intensity focused ultrasound (HIFU) from a laser-induced bubble in various water temperatures and dissolved oxygen (DO) has been investigated. A laser-induced bubble generated near the geometrical focus of HIFU is utilized to yield intense negative pressure by the backscattering. Optical observation with a high-speed video camera and pressure measurement with a fiber-optic probe hydrophone are conducted simultaneously to understand the forming process of a bubble cloud and corresponding pressure field by the backscattering.

Enzyme histochemical adaptive responses of the medial pterygoid muscle and two heads of the lateral pterygoid muscle to long-term soft diet feeding in growing rabbits.

The aim of the present study was to investigate the histochemical effects of long-term soft diet in the medial pterygoid muscle as well as the two heads of the lateral pterygoid muscles in growing rabbits. Eleven young rabbits were divided into two groups as solid diet (control group; n = 6) or soft diet (soft-diet group; n = 5) groups. After 6 months, muscle fibers from the medial and the two heads of the lateral pterygoid muscles were histochemically defined.

The effects of surfactant on the multiscale structure of bubbly flows.

It is well known that a bubble in contaminated water rises much slower than one in purified water, and the rising velocity in a contaminated system can be less than half that in a purified system. This phenomenon is explained by the so-called Marangoni effect caused by surfactant adsorption on the bubble surface. In other words, while a bubble is rising, there exists a surface concentration distribution of surfactant along the bubble surface because the adsorbed surfactant is swept off from the front part and accumulates in the rear part by advection.

Positive patch test for mercury possibly from exposure to amalgam.

Objectives: Mercury allergy is a serious health problem. We investigated the relationship between positive patch test for mercury and sources of mercury exposure, indicated by concentrations in biological samples from healthy medical students.

Methods: Patch tests for mercury (Hg-PT) were performed on 580 students.

Occult fractures of articular eminence and glenoid fossa presenting as temporomandibular disorder: a case report.

We report an unusual case of occult fractures of the articular eminence and glenoid fossa due to a previous traffic accident presenting as a temporomandibular disorder. A 24-year-old Japanese man was referred for trismus and pain in the right temporomandibular joint, and was suspected of having temporomandibular disorder. Although the magnetic resonance image did not show displacement of the articular disk, T2-weighted images revealed disruption of the cortical low-intensity line of the right articular eminence.

Diagnostic significance of FDG-PET and argyrophilic nucleolar organizer regions (AgNORs) in oral squamous cell carcinoma.

Neoadjuvant chemoradiotherapy has played an important role in preserving organs and functions in patients with oral squamous cell carcinoma (SCC). To determine whether further operation after chemoradiotherapy is necessary, accurate evaluation of residual tumour cells after treatment is essential. We investigated the usefulness of regional fluorodeoxyglucose (FDG) uptake in combination with histochemical expression of AgNORs obtained from pretreatment biopsy specimens to assess residual tumours after intraarterial chemoradiotherapy.

Preoperative assessment of the relationship between the mandibular third molar and the mandibular canal by axial computed tomography with coronal and sagittal reconstruction.

Objective: We sought to evaluate the relationship between the mandibular third molar and the mandibular canal by using axial computed tomography with coronal and sagittal reconstruction for third molar surgery.

Study Design: Forty-seven impacted third molars in 41 patients were found in close association with the mandibular canal during a panoramic radiographic assessment. The relationship between the mandibular third molar and the mandibular canal was evaluated by using computed tomography and compared in terms of operative exposure of the inferior alveolar nerve and postoperative labial dysesthesia.

Use of third molar transplantation for closure of the oroantral communication after tooth extraction: a report of 2 cases.

Objective: This clinical report introduces a promising and unique method for the immediate closure of the oroantral communication (OAC) after tooth extraction: the use of the transplanted third molar with closed apices.

Study Design: In 2 adult patients, OAC caused by the extraction of an upper molar was immediately closed by using a transplanted third molar with complete root formation. After tooth extraction at the recipient site, OAC with perforated mucosa of the sinus floor was confirmed and the donor third molar was transferred to the prepared recipient socket.

Prospective comparison of 18F-FDG PET with conventional imaging modalities (MRI, CT, and 67Ga scintigraphy) in assessment of combined intraarterial chemotherapy and radiotherapy for head and neck carcinoma.

Unlabelled: To preserve the oral organs and functions in patients with head and neck carcinoma, accurate determination of the appropriate treatment after neoadjuvant chemotherapy and radiotherapy is of critical importance. We evaluated the diagnostic accuracy of (18)F-FDG PET relative to that of other conventional imaging modalities in the assessment of therapeutic response after combined intraarterial chemotherapy and radiotherapy as an organ preservation protocol.

Methods: The study was prospectively performed on 23 consecutive patients with head and neck squamous cell carcinoma who completed the treatment regimen and underwent 2 (18)F-FDG PET studies before and after neoadjuvant chemoradiotherapy.

FDG-PET for prediction of tumour aggressiveness and response to intra-arterial chemotherapy and radiotherapy in head and neck cancer.

The aim of this study was to evaluate the possible usefulness of fluorine-18 fluorodeoxyglucose positron emission tomography (FDG-PET) for predicting tumour aggressiveness and response to intra-arterial chemotherapy (THP-ADM + 5-FU + carboplatin) and radiotherapy in head and neck carcinomas. Twenty patients with squamous cell carcinoma (SCC) of the head and neck were included in the study. All patients completed the treatment regimen, and each patient underwent two FDG-PET studies, one prior to and one at 4 weeks after the chemoradiotherapy.

Treatment of severe mandibular prognathism in combination with maxillary hypoplasia: case report.

We performed a Le Fort I osteotomy and sagittal split ramus osteotomy (Obwegeser-Dal Pont) combined with mandibular anterior segmental osteotomy without tooth extraction for a patient with severe mandibular prognathism accompanied by a hypoplastic maxilla, anterior open bite and normal anterior mandibular vertical dimension. The results of facial appearance and occlusion were excellent. This combined surgical method appears to be satisfactory for treating severe mandibular prognathism with hypoplastic maxilla.

Observation of molecular changes of a necrotic tissue from a murine carcinoma by Fourier-transform infrared microspectroscopy.

Purpose: Our purpose is to develop infrared (IR) microspectroscopy as a new optical diagnostic tool to support conventional lightscopic techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects of anticancer therapy by monitoring the IR spectra in the necrotic area.

Experimental Design: We evaluated the tissue which amassed for 4 weeks after the isotransplantation of mouse squamous cell carcinoma into the thigh of mice. The borders of the necrotic area of frozen tissue specimens were investigated by Fourier-transform IR microspectroscopy and conventional histological staining.

Whole-body (18)F-fluorodeoxyglucose positron emission tomography in patients with head and neck cancer.

Objective: Because of the high risk of secondary primary malignancies in addition to possible distant metastases, whole-body evaluation is critically important in patients with head and neck cancer. We evaluated the clinical usefulness of whole-body (18)F-fluorodeoxyglucose (FDG) positron emission tomography (PET) before initial treatment.

Study Design: We performed whole-body FDG PET in 26 patients with head and neck cancer (squamous cell carcinoma, n = 20; salivary gland carcinoma, n = 6) before initial treatment.