Clinical systemic lupeol administration for canine oral malignant melanoma.

Canine oral malignant melanoma (COMM) is the most aggressive malignant tumor in dogs. Lupeol is a triterpene extracted from various fruits and vegetables that reportedly inhibits melanoma cell proliferation and . In this study, the efficacy of subcutaneous lupeol for spontaneous COMM was evaluated.

Non-surgical treatment of canine oral malignant melanoma: A case study of the application of complementary alternative medicine.

This report describes a dog with a clinical stage III oral malignant melanoma that was treated with complementary alternative medicine (CAM). The CAM included high temperature hyperthermia, dendritic cell therapy and lupeol injections. Surgery, radiation and chemotherapy were not performed.

Yamabushitake mushroom (Hericium erinaceus) improved lipid metabolism in mice fed a high-fat diet.

The effects of dietary Yamabushitake mushroom (Hericium erinaceus) on lipid metabolism were examined. C57BL/6J mice were fed a high-fat diet containing hot-water extract (HW-E) and an ethanol extract (EtOH-E) of Yamabushitake mushroom. Administration of HW-E or EtOH-E with a high-fat diet for 28 d resulted in a significant decrease in body weight gain, fat weight, and serum and hepatic triacylglycerol levels.

Differentiation-inducing activity of lupane triterpenes on a mouse melanoma cell line.

Lupane triterpenes were found to promote melanogenesis, a hallmark of B16 2F2 mouse melanoma cell differentiation. Studies of the structure-activity relationships demonstrated that the keto function at C-3 of the lupane skeleton played important roles in the melanogenic activities of lupane triterpenes on melanoma cells. The carbonyl group at C-17 of lupane triterpenes was essential against their apoptosis-inducing activity against human cancer cells via the inhibition of topoisomerase I.

Lupane triterpenes with a carbonyl group at C-20 induce cancer cell apoptosis.

We evaluated the effects of various lupane triterpenes on B16 2F2 mouse melanoma cell differentiation and proliferation. All of the compounds tested (numbered 1-6) induced melanogenesis of B16 2F2 cells, a marker of melanoma cell differentiation. Compounds 4-6, which have a carbonyl group at C-20, markedly inhibited the growth of B16 2F2 cells by the induction of apoptosis.