1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum.

Background: There is a need for a pancreatic lipase (LIP) reference assay to provide an accurate base to which routine methods can be traceable.

Methods: This study developed a novel LIP assay method in which 1,2-dioleoylglycerol (DODG) is the substrate and LIP activity is measured in a coupled enzymatic reaction from the increase in absorbance at 340 nm with production of NADPH.

Results: With this method, LIP activity was linear up to 440 U/L (8-times expected upper limit of physiological concentration).