Sequential changes of Legionella antigens and bacterial load in the lungs and urines of a mouse model of pneumonia.

Legionella pneumophila is an important cause of community- and hospital-acquired pneumonia. In spite of the introduction of the urinary antigen detection method, Legionella pneumonia may be still underdiagnosed. We performed kinetic and quantitative analysis of diagnostic markers, such as bacterial loads, DNA assays, and antigen titers, in a 28-day time course murine model of L.

[Sensitive and rapid detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification].

For establishment of a method for rapid diagnosis of Mycoplasma pneumoniae in clinical specimens, we designed and evaluated a loop-mediated isothermal amplification (LAMP) assay, using a set of primers targeting the SDC1 repetitive element of the M. pneumoniae genome. The method showed rapid and specific amplification for all the strains of M.

[Rapid and simple detection of Legionella species by LAMP, a mew DNA amplification method].

Legionella is a common cause of community-acquired respiratory tract infections and occasionally causes nosocomial pneumonia. Rapid and accurate detection of legionellae is important for diagnosis and treatment of patients. In order to detect legionellae, a new DNA amplification method was designed and evaluated.