Publications by authors named "Toshihisa Sugino"

To identify new criteria for selecting recipient dairy cows for embryo transfer (ET), we retrospectively examined the effects of the composition of early lactation milk on fertility risk in ET. This study investigated the association between milk fat (FAT), milk protein (PRO), and milk urea nitrogen (MUN) levels during early lactation, based on production records, and subsequent fertility risk in ET using contingency table analysis and multivariable logistic regression analysis, which included five confounding variables. The results showed that MUN levels during early lactation were negatively associated with fertility risk in ET, while FAT and PRO levels showed no clear association.

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This study aimed to clarify the efficacy of cashew nutshell liquid (CNSL) in methane emissions, milk production, and rumen fermentation of lactating cows in practical conditions. Ten Holstein lactating cows were used in a free-stall barn with a milking robot. Two treatments were arranged as control (no CNSL additive, n = 5) or CNSL addition (10 g/day of CNSL, n = 5) for 21 days after the 7-day preliminary period.

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Various studies have attempted to improve the milk yield and composition in dairy animals. However, no study has examined the effects of milking at different times on milk yield and composition. Therefore, this study aimed to compare the yield, composition, and antimicrobial components of milk obtained from milking at different times in lactating goats.

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Previous studies have shown that a single infusion of lipopolysaccharide (LPS) into the uterus induces mammary gland inflammation. However, repeated LPS infusions return the mammary glands to their basal state of inflammation. To confirm that this is a state of tolerance to LPS, we examined whether tolerance induced by repeated intrauterine LPS infusions limits mammary gland inflammation following subsequent intramammary LPS infusions.

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We aimed to evaluate the effect of dietary calcium (Ca)-octanoate supplementation on concentrations of ghrelin, growth hormone (GH), insulin-like growth factor-1 (IGF-1), and insulin in plasma and milk of beef cattle during late gestation and early postpartum. Twelve Japanese Black cattle were offered concentrate without (CON, n = 6) or with Ca-octanoate supplementation at 1.5% of dietary dry matter (OCT, n = 6).

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  • This research aims to improve the quality of rice bran (RB) and de-oiled rice bran (DORB) by using fermentation with rumen microbes from sheep, leading to beneficial chemical changes.
  • The fermentation process involved various moisture levels and durations, resulting in a decrease in harmful compounds like phytate-P and crude fiber, while increasing valuable nutrients like inorganic phosphorus.
  • The study concludes that fermenting RB for 12 hours and DORB for up to 72 hours can make these byproducts more suitable as feed for non-ruminant animals like poultry and pigs, especially with moisture levels up to 50%.
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  • Sake lees, a byproduct of Japanese rice wine, are high in protein and ethanol and were studied as feed for sheep, focusing on their effects on rumen fermentation and digestion.
  • The study used a 4 × 4 Latin square design with four sheep, testing ensiled versus non-ensiled total mixed rations (TMR) with different types of sake lees.
  • Results showed that while dietary ethanol was higher for those on ensiled TMR, it negatively affected dry matter and nitrogen digestion, with lower plasma amino acid concentrations, indicating potential concerns when formulating such feeds.
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Like glucose, fructose is a monosaccharide, but the mechanisms of its absorption and metabolism in the body are very different between the 2 molecules. In this study, we investigated the effects of oral administration of glucose and fructose on food intake, diencephalic gene expression, and plasma metabolite concentrations in broiler chicks. The animals used in this study were 4-day-old male broiler chicks (Ross 308).

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We investigated the temporal dynamics of ruminal pH and the composition and predicted functions of the rumen bacterial community in Holstein cows during the periparturient period. Eight multiparous Holstein cows (body weight; 707.4 ± 29.

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  • * Eighteen dairy cows were split into control and KP groups, with the KP group adapting to the new feed three weeks before calving; both groups showed similar dry matter intake, body weight, and milk yield after 12 weeks.
  • * The KP diet led to higher rumen pH, lower temperature, and changes in fatty acid proportions without harming productivity, while also improving nutrient digestibility and reducing urinary nitrogen excretion.
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  • The study investigated the impact of different forage sources (Italian ryegrass silage vs. corn silage) on milk production and phytanic acid content in milk from dairy cows.
  • Cows were fed a total mixed ration (TMR) containing either forage type over three 21-day periods, revealing no significant differences in overall milk composition or blood plasma markers across the periods.
  • Results showed that milk phytanic acid content was highest during the period when cows consumed ryegrass silage, indicating that diet might influence the conversion of dietary phytol to phytanic acid in milk, though the overall conversion rate was low.
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We examined the effects of oral administration of L-citrulline (L-Cit) on plasma metabolic hormones and biochemical profile in broilers. Food intake, water intake, and body temperature were also analyzed. After dual oral administration (20 mmol/head/administration) of L-Cit, broilers were exposed to a high ambient temperature (HT; 30 ± 1°C) chamber for 120 min.

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We investigated changes in rumen fermentation, peripheral blood metabolites and hormones, and hepatic transcriptomic dynamics in Holstein cows with and those without subacute ruminal acidosis (SARA) during the periparturient period. Sixteen multiparous Holstein cows were categorized in the SARA ( = 8) or non-SARA ( = 8) groups depending on whether they developed SARA during the 2 wk after parturition. Reticulo-ruminal pH was measured continuously throughout the study.

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  • The study assessed how ensiling (fermenting) fruit byproducts like persimmon peel and grape pomace impacts their chemical makeup and fermentation in sheep's stomachs (rumen).
  • It found that ensiling reduced non-fiber carbohydrates, especially in persimmon peel, but total tannins remained stable up to 4 weeks, with some soluble tannins decreasing.
  • The ensiled byproducts generally led to lower gas and fatty acid production, but adding polyethylene glycol (PEG) helped increase gas production, indicating that the effects of ensiling on fermentation may vary between different fruit byproducts.
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  • The study examined how subacute ruminal acidosis (SARA) affects blood metabolites, oxidative stress markers, and gene expression in Holstein cows around the time of giving birth.
  • Eighteen cows were divided into two groups: SARA (cows with low ruminal pH) and non-SARA, with various blood and liver samples taken before and after parturition.
  • Results showed significant changes in oxidative stress markers and gene expression in the SARA group, indicating a potential link between negative energy balance and increased oxidative stress during the periparturient period.
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The effects of inclusion of persimmon peel (PP) in total mixed ration (TMR) silage on its nutrient composition, tannin content, and in vitro ruminal fermentation were studied. Four types of TMR silages containing 0, 50, 100, and 150 g/kg of PP on a dry matter basis were prepared. The dietary contents of non-fiber carbohydrate (NFC) decreased, while soluble protein fraction increased after ensiling of the TMR.

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  • The study investigated the impact of colostrum supplementation on antimicrobial peptides in the milk of peripartum goats.
  • Pregnant goats were given colostrum whey products or water daily for four weeks surrounding the time of giving birth, and various samples were collected for analysis.
  • Results showed that colostrum supplementation increased IgA and lactoferrin in the milk, which may enhance the health of their kids, while it led to lower levels of another peptide, S100A7.
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The effect of blue LED on melatonin secretion, feeding behaviour and growth was addressed in Holstein female dairy calves. In Exp.1, six animals (8 weeks old, 97 ± 4.

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The authors have elected to retract this paper in accordance with the following points. The article as written contains misleading information and omits important details. Cows in this study were assigned to groups based on the current definition of subacute ruminal acidosis; they were housed on two different farms and fed two different sets of rations in this study.

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This study aimed to investigate the effect of an ensiling period (Experiment 1) and adding lactic acid bacteria (LAB, Experiment 2) on the changes in carotenoid, chlorophyll, and phytol in ensiled Italian ryegrass (IR, Lolium multiflorum Lam.). In Experiment 1, the IR herbage ensiled into plastic bags was analyzed for the contents of photosynthetic pigments and phytol over a 5-week period.

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The objective of this study was to evaluate the effect of tributyrin (TB) supplementation to milk replacer (MR) on performance, health, and blood concentrations of metabolite and glucagon-like peptide (GLP-2) in pre-weaning calves. Twenty Holstein heifer calves were raised on an intensified nursing program using MR supplemented with either palm oil (CON) or TB (TB) at 0.3% (as fed basis) for 7 weeks starting 1 week after birth.

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  • Three rumen-protected lysine (RPL) products were tested for stability in two different forage-based total mixed rations (TMR), focusing on their lysine release over time.
  • In the experiments, the RPL products showed varying levels of lysine release percentage based on the diet's dry matter (DM) content and the duration of exposure, with the third product (C) performing the best.
  • The study also found a correlation between the lysine release in water and the diets, suggesting that Brix value could be a useful indicator for assessing the effectiveness of RPL protection.
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  • Butyrate has been shown to improve gut development and growth in calves, while GLP-1 and GLP-2 play important roles in regulating blood sugar and gut health.
  • * The study aimed to investigate how different doses of butyrate infused into the rumen of dairy calves affect the levels of GLP-1 and GLP-2 in their plasma.
  • *Results indicated that increasing butyrate doses led to higher concentrations of ruminal and plasma butyrate and total volatile fatty acids, along with variations in GLP-1 and GLP-2 levels after treatment.
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  • Single amino acids (AA) feeding is being studied for their effects beyond just nutrition, particularly their impact on endocrine and metabolic responses in sheep after fasting.
  • Fasted sheep were given a duodenal infusion of saline or specific AAs (glutamate, glutamine, lysine, threonine, valine) over four hours, while blood samples were collected to measure various metabolic markers.
  • Findings indicated that certain AAs enhanced ghrelin release, altered levels of plasma BHBA and NEFA, increased insulin and glucagon levels, and elevated IGF-1, suggesting that selective AA feeding can improve endocrine response and help manage fat mobilization during feed restriction.
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