Preparation and properties of liposomes composed of various phospholipids with different hydrophobic chains using a supercritical reverse phase evaporation method.

Liposomes were prepared by the supercritical reverse phase evaporation method developed in our laboratory using various phospholipids with different hydrocarbon chains. The effects of the length of alkyl chain and number of unsaturated bonds of phospholipids on the properties of liposomal membranes were examined through trapping efficiency measurements, transmission electron microscopic observations, and osmotic response measurements. The trapping efficiency for water-soluble drugs of liposomes prepared by our method was greatly higher than that of liposomes prepared by the conventional Bangham method.

Observation of the ocular fundus by an infrared-sensitive video camera after vitreoretinal surgery assisted by indocyanine green.

Purpose: To investigate how long indocyanine green (ICG) dye remains in the ocular fundus and how the remaining dye interferes with ICG angiographic findings after vitreoretinal surgery assisted by ICG.

Methods: Eight eyes with macular hole, three with epiretinal membrane, and one with an intraocular foreign body were included. Those patients underwent vitrectomy facilitated by an intraocular administration of ICG.