Changes in Histamine/Tryptase Levels in Skin Chambers: Application for Clinical Evaluation of Atopic Dermatitis.

We measured changes in histamine and tryptase levels in fluid of children with atopic dermatitis using skin chamber methods and evaluated the correlation with clinical symptoms. Skin chambers were applied to forearm skin which had been scratched with a needle, and an extract of Dermatophagoides farinae (mite antigen) at 50 μg/ml in saline was added through 0.3-μm filters as a challenge.

Bronchial Response Study of Asthmatic Patients Using an Atmosphere-Changing Chamber.

We studied the bronchial response to low-pressure conditions by using an atmosphere-controlling chamber. When asthmatic patients were put in a chamber in which the pressure was reduced gradually, their small airways did not change until an estimated 1,000-meter level and from an estimated 1,500 m asthmatic patients' lung function crossed to that of normal subjects. For astmatics there was a remarkable change in saturation of oxygen under low-pressure conditions.