Phylogenetic analysis of Fusobacterium necrophorum, Fusobacterium varium and Fusobacterium nucleatum based on gyrB gene sequences.

The nucleotide sequences of the DNA gyrase B subunit gene (gyrB) of Fusobacterium necrophorum subsp. necrophorum, F. necrophorum subsp.

Construction and in vitro properties of chimeric simian and human immunodeficiency virus with the human TNF-alpha gene.

Tumor necrosis factor-alpha (TNF-alpha) has been reported to be involved in the development and progression of acquired immunodeficiency syndrome (AIDS). To study the role of this cytokine in AIDS pathogenesis, we constructed a chimeric simian and human immunodeficiency virus (SHIV) having the human TNF-alpha gene (SHIV-TNF) and characterized its properties in vitro. SHIV-TNF replicated both in M8166, a human T cell line, and in monkey peripheral blood mononuclear cells (PBMCs).

Specific detection and differentiation of two subspecies of Fusobacterium necrophorum by PCR.

The phylogenic relationships of two subspecies of Fusobacterium necrophorum were investigated by randomly amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR). With each of the 12 random primers, the DNA fingerprints generated were subjected to cluster analysis for dendrograms. The analysis indicated that twelve strains were organized into two major clusters, and that all strains of each subspecies were confined to one cluster.

[Pathogenicities of Mycobacterium intracellulare and M. avium strains to the mice which were isolated from non-tuberculous mycobactriosis patients].

The virulence of 5 strains of M. intracellulare and 6 strains of M. avium to mice were examined.

[The influence of urea, ammonia and nitrate as they affect growth and viability of Trichophyton verrucosum].

Six strains of Trichophyton verrucosum were used in a test to determine effects of urea and sodium nitrate on their growth. Despite having urease activity, the strains failed to utilize the urea and sodium nitrate for their growth. Moreover, the organisms were killed if large amounts of these nitrogen compounds and ammonia were added to the medium.

Comparison of the 16S-23S rRNA intergenic spacer regions between Fusobacterium varium and "Fusobacterium pseudonecrophorum" strains.

The 16S-23S rRNA intergenic spacer regions (ISRs) of five strains of "Fusobacterium pseudonecrophorum" which had been proposed as a new species, were compared with those of F. varium ATCC 8501T. All the strains of "F.

Characterization of the 16S-23S rRNA intergenic spacer regions among strains of the Fusobacterium necrophorum cluster.

The 16S-23S rRNA intergenic spacer regions (ISRs) of Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp.