Publications by authors named "Toshie Kakinuma"
Saving space for sperm cryopreservation would aid mouse genetics research. We previously developed the ST (sperm freezing in ShorT STraw to reduce STorage space) method for cryopreserving mouse sperm in a smaller storage space than conventional methods. However, our ST method has two drawbacks: difficulties during freeze-thaw procedures and the potential risk of sperm loss during storage.
View Article and Find Full Text PDFEfficient cryopreservation and transportation of mouse sperm are among the most desirable strategies for current and future research on mouse genetics. However, the current method for sperm cryopreservation uses an 11-cm plastic straw, which is a bulky and fragile container. Developing an alternative to overcome the limitations associated with this method would accelerate biomedical research.
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- A minimum number of healthy embryos is necessary to support pregnancy in mice, leading to the use of cotransfer techniques to increase embryo viability.
- In this study, researchers compared the effects of cotransfer between C57BL/6J (B6) embryos and two white coat-colored strains, SJL/J and ICR, on pregnancy outcomes.
- Results indicated similar pregnancy maintenance and litter sizes for both strains, but B6 pups showed better postnatal growth when cotransferred with SJL/J pups, highlighting potential competition issues between littermates.
Supplementation with oxygen is fundamental in rescue and emergency medicine. However, transportation of oxygen cylinders or a rigid hyperbaric chamber requires large work forces. Also, oxygen in a cylinder may be completely consumed during a rescue action.
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