[In vitro cell adhesion and integrin expression by calcium ionophore-treated mononuclear cells from patients with acute myeloid leukemia].

As shown elsewhere, cultured acute myeloid leukaemia blasts acquire certain characteristics of dendritic cells upon stimulation with cytokines and calcium ionophore. The ability of leukaemia-derived dendritic-like cells to express immune costimulatory molecules and dendritic cell marker CD83 has been extensively investigated. Although migratory capacity is a major attribute of dendritic cells, the ability of in vitro modified blasts for adhesion, chemotaxis and homing remain elusive.

Adhesion capacity and integrin expression by dendritic-like cells generated from acute myeloid leukemia blasts by calcium ionophore treatment.

Objective: Dendritic cells (DC) play a key role in initiation of immune responses. In vitro modified acute myeloid leukemia (AML) blasts acquire certain specific features of DC and are suggested as a potential source of anti-leukemia vaccines. AML-DC have been characterized in terms of costimulatory molecule expression and cytokine production.

[Hematopoietic cells of fetal liver. 1. Effect of growth factors of varying origin and activity of granulocyte-macrophage progenitor cells].

The liver is the leading hemopoietic organ of 5-22-week embryo and fetus. Hepatic hemopoiesis activity is assessed by concentration, total count and proliferative capacity of granulomonocytic cell precursors (GMCP). Cloning efficacy (GMCP CE) in the liver of 7 fetuses of 16-22 weeks of gestation was evaluated in vitro.

[Expression of glucocorticoid receptors and blast cell clearance in children with different variants of acute lymphoblastic leukemia].

Glucocorticoid receptor (GR) levels were estimated in blast cells of 24 children with acute lymphoblastic leukemia, in different immunocytologic subvariants of the disease. No relationship was revealed between immunophenotype and GR number in blasts. It was found that the response to prednisolone therapy did not depend on the number of sites specifically bound to glucocorticoids.

[Antiprolifirative effect of chlorbutin and sensitivity of patients with glomerulonephritis to this drug].

Children suffering from different forms of glomerulonephritis were examined for the sensitivity of peripheral blood lymphocytes to the antiproliferative action of chlorambucil in vitro. All the patients were given chlorambucil treatment in accordance with a 4-component scheme. As a result, in 15 patients (group I), a complete clinicolaboratory remission was attained.

[Membrane markers and interleukin-2 receptor of splenic lymphocytes in children with aplastic anemia: detection of active subpopulations].

A total of 16 children with aplastic anemia were investigated, among them 6 girls and 10 boys, the mean age 10.5 years. Splenectomy was included into the complex of treatment along with glucocorticoid and transfusion-replacement therapy of all the children.

[Immunophenotype of peripheral blood lymphocytes in children living in radiation-polluted territories].

The authors provide the data on the assessment of the population composition of peripheral blood lymphocytes. The assessment was performed with the aid of antilymphocytic monoclonal antibodies in children populating radioactive cesium polluted territories and exposed to ionizing radiation in small doses. 56 children were examined.

[Circulating granulomonocytic precursor cells and phenotype of peripheral blood monocytes in premature infants].

The functional state of granulomonocytic hemopoietic stem cells was investigated in 72 premature infants in the neonatal period. New data were obtained on the concentration of committed granulomonocytic precursors, and on the characteristics of the phenotype of monocytes--the final differentiation component of this series. These data have verified the present notions on the features of nonspecific adaptation reactions in premature newborns.

[Effects of glucocorticoid therapy on membrane markers of lymphocytes and monocytes in children with chronic idiopathic thrombocytopenic purpura].

The treatment with glucocorticoids brings about a decrease in the expression of Ia-like and LFA-J antigens on the membrane surface of peripheral blood lymphocytes and monocytes, diminution of the relative count of monocytes with fixed immunoglobulins (with Fc-receptors). A reduction of the count of B lymphocytes and monocytes with Ia and LFA-J antigens in the spleen was recorded. The hematologic effect attained in the treatment with glucocorticoids was determined by the combined effect of the drugs: by the blockade of monocyte/macrophage receptors and by the redistributiion++ action of prednisolone leading to the release of blood platelets from the bone marrow to the blood bed.

[Pathogenesis of immunologic deficiency in the initial stage of lymphogranulomatosis in children].

Antilymphocytic monoclonal antibodies of HKO and OKT series were used to study surface antigens of peripheral blood and lymph node lymphocytes in 13 untreated patients with Hodgkin's disease. The purpose of the study was to identify the pathogenetic mechanism underlying the development of immunodeficiency in the early disease stages. The data obtained have demonstrated that the deficiency of T lymphocytes in the peripheral blood was connected with that of mature T cells in the lymph nodes revealed with the use of differential antigens complex.

[Immunocytological characteristics of primary lesions of the lymph nodes in children with lymphogranulomatosis].

Antilymphocytic monoclonal antibodies of UKO and OKT series were used to study surface lymphocytic antigens in 40 primarily affected lymphatic nodes in 19 children with lymphogranulomatosis. In 17 lymphatic nodes immunological reaction was determined as the T-type (OKT3+--42.3 +/- 2.

[The expression of lymphocytic function-associated antigen determined by IKO-11 monoclonal antibodies in patients with nephroblastoma and neuroblastoma].

The importance of NK-cells was shown in 12 patients with nephroblastoma and 10 patients with neuroblastoma using ICO-11 monoclonal antibodies. The numbers of circulating LFA-1+ cells were shown to be different in the untreated and treated patients. A certain regular influence of antitumour therapy on the amount of circulating LFA-1+ cells was established.

[Clonogenic fibroblasts from the hematopoietic organs of the human embryo and fetus at different gestational ages].

Fibroblast precursors of hemopoietic organs of 72 embryos and fetuses 5-27 weeks of age have been studied. The study has shown that the increase in the number of clonogenic fibroblasts took place in the bone marrow and spleen 2-3 weeks before the beginning of hemopoiesis, that is during the period of the highest hemopoietic stem cell concentration. These data suggest possible participation of stromal fibroblasts of hemopoietic organs in the formation of microenvironment for hemopoietic stem cell functioning.

[Surface membrane differentiation antigens of the lymph node lymphocytes in nonspecific lymphoproliferative reactions and lymphogranulomatosis in children].

Monoclonal antibodies ICO and OKT, OKM5, C-ALLA have been used to investigate surface lymphocyte antigens in normal (9), reactive (27) lymph nodes and in lymphogranulomatosis (9) in children. The study has shown that the number of lymphocytes with T and B markers is 45% in normal lymph nodes; OKT4/OKT8 is 0.9.

Clinical effect and some pathogenic mechanisms of high dose monomeric IgG therapy in childhood chronic idiopathic thrombocytopenic purpura.

8 children with chronic idiopathic thrombocytopenic purpura received a high dose therapy with monomeric IgG. Before and after the treatment immunological investigations were carried out. All the children showed a positive clinical response, in 5 children there was an increase of thrombocytes to 111-340 X 10(9)/l.