NMR study of the gelation of a designed gelator.

The gelation of a designed gelator was investigated by different NMR methods, which showed a clear thermal hysteresis. Two very simple approaches for the NMR determination of the gelation point are suggested. One involves the observation of the NMR integral, and the other records the ratio of the diffusion coefficients between the gelator and the solvent.

Residue-specific NH exchange rates studied by NMR diffusion experiments.

We present a novel approach to the investigation of rapid (>2s(-1)) NH exchange rates in proteins, based on residue-specific diffusion measurements. (1)H, (15)N-DOSY-HSQC spectra are recorded in order to observe resolved amide proton signals for most residues of the protein. Human ubiquitin was used to demonstrate the proposed method.

Diffusion NMR as a new method for the determination of the gel point of gelatin.

The gelation of gelatin has been investigated using pulsed field gradient (PFG) NMR. For the first time, diffusion results have been used to determine the gelation point, which is indicated by a minimum in the self-diffusion coefficient of the free polymer fraction vs temperature. Biexponential analysis of the diffusion decay data allowing the diffusion of free and network-bound gelatin to be determined separately has been applied to provide an extended insight into the gelation process.