[Preparation of Pseudomonas aeruginosa recombinant outer-membrane protein F and the study of its antigenic properties].

In Escherichia coli M15, the gene of P. aeruginosa recombinant outer-membrane protein F (OprF) was cloned. OprF, chromatographically purified on Ni-agarose and containing an additional sequence of 6 histidines on the N-end, was obtained.

[Analysis of 99mTc-HIDA kinetics using an adaptive model].

The paper is concerned with the results of assaying hepatocyte function on the basis of an adaptive pharmacokinetic model worked out by the authors. Hepatoscintigraphy with 99mTc-HIDA was used in 3 phases: the vascular phase of RP transport (the 1st min of investigation, scintigrams with a 2 s interval), the parenchymatous phase (with a 20 s interval in subsequent 10 min), the biliary phase (with a 3 min interval). The hepatocytic phase of 99mTc-HIDA transport was evaluated on the basis of the adaptive 5-compartmental model calculating the value of transport constants.

[Study of a multifield equivalent cardiac generator].

The electric field of the isolated dog heart immersed into a liquid volumetric conductor was subjected to a thorough investigation. Measurements and subsequent calculations showed that in precordial leads the quantitative description of the cardiac generator by a multipole equivalent generator of the 1st order (dipole) is rough approximation. Addition of a multipole component of the 2nd order (quadripole) to the equivalent generator increases but relatively little the accuracy of description.