Publications by authors named "Toptikov V"

The relevance of comprehensive studies of the Rapana vital functions is determined by its considerab­le negative impact on the ecosystem of the Black Sea. The aim of the work was to find out the polymorphism and activity of the main hydrolases in the different parts of the digestive system of Rapana. Hydrolases (proteases, amylases, esterases, lipases and phosphatases) in glandular structures of the Rapana digestive system were studied by electrophoresis.

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The present review devoted to the analysis of recent literature on genetic determination and the domain organization of the newly discovered two-component signaling systems in pro- and eukaryotes. These structures are involved in the regulation of numerous morphological and physiological processes in plants. RR-proteins, it the key elements of signaling systems, they launch a cascade of phosphotransferase reactions and directly or indirectly regulate the transcription and activity other proteins, including enzymes, in response to hormones or environmental factors.

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Cultivation of wheat and barley plants under temperature stress fluctuations was the reason of reduction of acid protein common storage in the tissues more than 25% and the reason of reduction of peroxidase and superoxide dismutase common activities. There were no changes of common phenoloxidase activity. Activity redistribution between separate fractions of enzyme spectra were linked with enzyme expression changes.

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Comparative estimation of the most widely used indexes of the system diversity has been carried out using model electrophoretic spectra. The basic requirements to the diversity index of electrophoretic spectra are formulated. A formula for the estimation of the level of spectra diversity of multiple forms on the basis of synthesis of the Ashby complication of the system index and the Simpson index is offered.

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In the review the problems of restoration of genetic coadaptation in some agricultural crops under creation of synthetic genotypes using sexual hybridization and transgenosis are considered. On the basis of the study of multiple molecular forms of the enzymes, the mechanisms of formation of adaptive complexes of genes (ACG) are traced. Cytogenetic mechanisms interfering stabilization of synthetic genotypes are considered.

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Potato plants of various cultivars, transformed using Agrobacterium tumefaciens with pGV941 plasmid, differed from control plants in glyphosate herbicide tolerance, tryptophane content, intensity of callusogenesis, microtuber formation in vitro and multimolecular forms of peroxidase (EC 1.11.1.

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The effect of different reagents to colour intensity on PAA gel bands using benzidine was studied. All the investigated reagents were divided into three groups 1) reagents with no effect on visualization of peroxidase molecular forms colour intensity; 2) those relatively equally decreasing colour intensity; 3) selective effect on visualization of peroxidase molecular forms. Ferulic, ascorbic and indole-3-acetic acids, phloroglucin and hydroquinone are most interesting in our investigation.

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