Publications by authors named "Toomas A Leinsoo"
Article Synopsis
- The nascent polypeptide-associated complex (NAC) is a crucial ribosome-associated protein involved in protein folding and sorting, and it is conserved across eukaryotic organisms.
- Researchers have identified germline-specific NACαβ paralogs (gNACs), which have unique protein structures in their α and β subunits, particularly longer regions that may be phosphorylated.
- The study suggests that these gNACs play a role in the coordination of protein regulation within germline cells, and there appears to be an interaction between gNACs and the commonly expressed NAC subunits that impacts organismal development.
Article Synopsis
- The nucleolus is not just for ribosome biogenesis; it houses proteins that help regulate the cell cycle and stress responses, including the piRNA binding protein Piwi, which silences transposable elements in Drosophila gonads.
- Research on ovarian somatic cells showed that Piwi's location shifts between the nucleoplasm and the nucleolus, with its retention in the nucleolus linked to specific nucleolar transcripts.
- Stress, particularly heat shock, prompts Piwi to relocate to the nucleolus, suggesting that certain transcripts produced during stress help sequester proteins, impacting the silencing of retrotransposon-containing rDNA copies.
Unlabelled: The main hypothesis suggested that changes in the external mechanical load would lead to different deformations of the submembranous cytoskeleton and, as a result, dissociation of different proteins from its structure (induced by increased/decreased mechanical stress). The study subjects were fibers of the soleus muscle and cardiomyocytes of Wistar rats. Changes in external mechanical conditions were reconstructed by means of antiorthostatic suspension of the animals by their tails for 6, 12, 18, 24 and 72 hours.
View Article and Find Full Text PDFRodent lymphocytes express functionally active glutamate receptors.
Biochem Biophys Res Commun
November 2004
RT-PCR demonstrated that ionotropic (iGluR NR1) and metabotropic (mGluR Group III) glutamate receptors are expressed in rodent lymphocytes. Flow cytometry showed that activation of iGluR NR1 by N-methyl-D-aspartate (NMDA) increased intracellular free calcium and reactive oxygen species (ROS) levels and activated caspase-3. The latter effect was attenuated by the NMDA antagonist, 5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine (MK-801), by the antioxidant N-acetylcysteine and by cyclosporin A.
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