Contrast-Enhanced X-Ray Micro-Computed Tomography as a Versatile Method for Anatomical Studies of Adult Zebrafish.

One attractive quality of zebrafish as a model organism for biological research is that transparency at early developmental stages allows the optical imaging of cellular and molecular events. However, this advantage cannot be applied to adult zebrafish. In this study, we explored the use of contrast-enhanced X-ray micro-computed tomography (microCT) on adult zebrafish in which the organism was stained with iodine, a simple and economical contrasting agent, after fixation.