Impact of waxy protein deletions on the crystalline structure and physicochemical properties of wheat V-type resistant starch (RS).

This study investigated the effects of waxy (Wx) protein on wheat V-type resistant starch (RS) formation, molecular structure, and physicochemical properties. We discovered that waxy protein deletions led to a rise in B- and C-type starch granules, while reducing A-type starch granules, amylose, and slowly digestible starch contents. Further, dodecyl gallate (DG) addition significantly increased RS content, and molecular dynamics simulations indicated that amylose and DG can form stable complexes.

An Air-Stable Carbon-Centered Triradical with a Well-Addressable Quartet Ground State.

Organic polyradicals with a high-spin ground state and quantum magnetic properties suitable for spin manipulation are valuable materials for diverse innovative technologies, including quantum devices. However, the typically high reactivity and low stability of conventional polyradicals present a major obstacle to such applications. In this study, a highly stable carbon-centered triradical with a quartet ground state and excellent stability (τ of ∼90 days in air-saturated toluene at room temperature) is achieved, which shows apposite magnetic anisotropy and Zeeman splitting partition with favorable addressability.

Overexpression of Wheat Selenium-Binding Protein Gene Enhances Plant Growth and Grain Selenium Accumulation under Spraying Sodium Selenite.

Selenium (Se) is an essential trace element for humans. Low concentrations of Se can promote plant growth and development. Enhancing grain yield and crop Se content is significant, as major food crops generally have low Se content.

Rational Design of an Air-Stable, High-Spin Diradical with Diazapyrene.

Stable carbon-based polyradicals exhibiting strong spin-spin coupling and slow depolarization processes are particularly attractive functional materials. A new molecular motif synthesized by a convenient method that allows the integration of stable, high-spin radicals to (hetero)aromatic polycycles has been developed, as illustrated by a non-Kekulé diradical showing a triplet ground state with long persistency (τ ≈31 h) in air. Compared to the widely used 1,3-phenylene, the newly designed (diaza)pyrene-4,10-diyl moiety is for the first time demonstrated to confer ferromagnetic (FM) spin coupling, allowing delocalized non-disjoint SOMOs.

Epidemiological Characteristics and Factors Associated with Alzheimer's Disease and Mild Cognitive Impairment among the Elderly in Urban and Rural Areas of Hubei Province.

Utilize the prevalence, associated factors and population distribution of AD and MCI among residents of the Hubei province aged 60 years or over to prove that elderly people who study and communicate with others, take part in regular physical exercise and choose a healthy lifestyle, will prevent or slow the decline in cognitive ability. If elderly people study and communicate with others, take part in regular physical exercise and choose a healthy lifestyle, can prevent or slow the decline in cognitive ability. A cross-sectional study was used for the recruitment of subjects.

Structure and Magnetism of an Ideal One-Dimensional Chain Antiferromagnet [CNH][Fe(SO)] with a Large Spin of = 5/2.

Isolated large-spin Heisenberg antiferromagnetic uniform chain is quite rare. Here, we have successfully synthesized an ideal one-dimensional (1D) = 5/2 linear-chain antiferromagnet [CNH][Fe(SO)], which crystallizes in a trigonal lattice with the space group 3. A broad maximum at = 18 K is observed in the magnetic susceptibility curve.

Occurrence and characterization of Shiga toxin-producing Escherichia coli (STEC) isolated from Chinese beef processing plants.

In order to investigate the prevalence, O serogroup, virulence genes and antibiotic resistance of Shiga toxin-producing Escherichia coli (STEC) in two beef plants in China, a total of 600 samples collected from 6 sites (feces, hide, pre-evisceration carcasses, post-washing carcasses, chilled carcasses and meat, 50 samples per site in each plant) were screened for the existence of Shiga toxin-encoding genes by PCR. STEC strains in positives were isolated and characterized for serogroup and antibiotic sensitivity. The PCR prevalence rate in each site was 45.

Probing the Axial Distortion Effect on the Magnetic Anisotropy of Octahedral Co(II) Complexes.

Three mononuclear octahedral Co(II) complexes are reported, [Co(py)(SCN)] (), [Co(py)(Cl)]·HO (), and [Co(py)(Br)] (), that exhibit different distortions with compression () or elongation ( and ) of the axial positions. Easy plane magnetic anisotropy was confirmed by magnetic, HF-EPR, and computational studies for all complexes. Further analyses indicate that both the sign and magnitude of zero-field splitting parameters experience a significant change ( ≥ ±150 cm) by tuning of the axial and equatorial ligand field strength.

Residual Negative Pressure in Vacuum Blood-Collection Tube and Hemolysis in Pediatric Blood Specimens.

Objective: To determine a method to reduce specimen hemolysis rates in pediatric blood specimens.

Methods: A total of 290 blood specimens from pediatric patients were classified into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer.

Residual Negative Pressure in Vacuum Tubes Might Increase the Risk of Spurious Hemolysis.

Background: We planned a study to establish whether spurious hemolysis may occur when negative pressure remains in vacuum tubes.

Methods: Four tubes with different vacuum levels (-54, -65, -74, and -86 kPa) were used to examine blood drawn from one healthy volunteer; the tubes were allowed to stand for different times (1, 2, 3, and 4 hours). The plasma was separated and immediately tested for free hemoglobin (FHb).

Designing and evaluating autoverification rules for thyroid function profiles and sex hormone tests.

Purpose Following the analytical phase, the current practice of many hospital laboratories involves the manual verification of all test results followed by the production of the report. However, manual verification is a time-consuming and tedious process. In this paper, we provide a detailed description of how to design autoverification rules for thyroid function test profiles and sex hormones.

Development and Implementation of Autoverification Rules for ELISA Results of HBV Serological Markers.

Autoverification is a process of using computer-based rules to verify clinical laboratory test results without manual review. But to date, there are few published articles on the use of autoverification over the course of years in a clinical laboratory. In our study, we firstly described the development and implementation of autoverification rules for enzyme-linked immunosorbent assay (ELISA) results of hepatitis B virus (HBV) serological markers in a clinical immunology laboratory.