EFFECT OF IMMERSION IN WATER ON DEFORMATION WITH A VARIETY OF DENTURE BASE MATERIALS AND MANUFACTURING TECHNIQUES.

Purpose: To evaluate the effect of immersion in water on deformation of maxillary denture bases created with CAD/CAM and heat-polymerized resin fabrication techniques under loading condi8ons.

Materials And Methods: 2-mm-thick denture bases were fabricated using four techniques: CAD/CAM milling (CCM; Polywax PMMA blank, Yamachi PMMA disk Yamahachi Dental); 3D prin8ng (3DP; BV005, NextDent Base); compression molding (CM; Luciton 199, Dentsply Sirona); and injec8on molding (IM; Ivobase, Ivoclar; n = 5 per group). Three static 49-N loads were applied perpendicular to each denture base, and the mean strain value (MSV) was recorded with strain gauges a[ached to the denture surface.

The analysis and feedback from the performance of pre-clinical operation skills tests for clerkship in School of Dentistry, National Taiwan University in 2022.

Background/purpose: The simulated pre-clinical operation skill test (PCOST) is part of the objective structured clinical examination (OSCE) and designed for clinical practice using manikins, physical typodonts, and instruments in the laboratory. This study tried to evaluate the influencing factors of the PCOST results for dental students.

Materials And Methods: The 32 5th-year dental students of the dental school of National Taiwan University (NTU) joined the PCOST.

The multi-factor analysis of computer-based test scores in objective structured clinical examination of clerkship in school of dentistry, National Taiwan University.

Background/purpose: The objective structured clinical examination (OSCE) is a test designed to test technical, clinical, and practical competency in medicine teaching. This study tried to evaluate the influencing factors of the computer-based tests (CBT) results for dental students.

Materials And Methods: The 32 5th-year dental students of the dental school of National Taiwan University (NTU) joined preclinical CBT.

Evaluation of sawbones training protocol in bone quality classification using tactile sensation.

Background/purpose: Bone quality may affect the implantation protocol. This study aimed to assess whether training protocols could improve novice dental practitioners' abilities in judging bone densities with tactile sensation.

Materials And Methods: Twenty-five operators were recruited to evaluate the density of artificial polyurethane bone blocks by a 2-mm twist-drill drilling and reported the bone quality perceived in a 100-mm VAS line.

Resolution of Excessive Interocclusal Restoration Space Post-Mandibulectomy Using a Two-Layer Retrievable Fixed Implant-Supported Prosthesis: A Case Report.

Patients treated with segmental mandibulectomy often require complicated rehabilitation. Maintenance of mandibular continuity and provision of adequate soft and hard tissue volumes are two key factors required for good clinical outcomes. Moreover, excessive interocclusal restoration space is a common problem in these patients.

Effect of scan delay on measurements of an occlusal pressure sensitive film: An in-vitro study.

Background/purpose: While scan delay may affect the measurements of an occlusal pressure-sensitive film, Dental Prescale II (DPS2), the duration of scan delay was rarely reported in previous studies. This study aimed to clarify the effect of scan delay on DPS2 measurements.

Materials And Methods: Two experiments were performed to clarify the effect of 0- to 10-min scan delay after DPS2 force registration.

Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.

Aim: To investigate the effects of butyric acid (BA), a metabolic product generated by pulp and root canal pathogens, on the viability and intercellular adhesion molecule-1 (ICAM-1) production of endothelial cells, which are crucial to angiogenesis and pulpal/periapical wound healing.

Methodology: Endothelial cells were exposed to butyrate with/without inhibitors. Cell viability, apoptosis and reactive oxygen species (ROS) were evaluated using an MTT assay, PI/annexin V and DCF fluorescence flow cytometry respectively.

Dimensional changes of complete dentures fabricated by milled and printed techniques: An in vitro study.

Statement Of Problem: Information on the dimensional changes in maxillary and mandibular dentures made by using computer-aided design and computer-aided manufacturing (CAD-CAM) techniques under uniform testing conditions is lacking.

Purpose: The purpose of this in vitro study was to evaluate the dimensional changes and reproducibility of maxillary and mandibular dentures by using CAD-CAM-milled and 3D-printed techniques.

Material And Methods: Maxillary and mandibular edentulous models with wax occlusal rims were scanned, and dentures were designed by using a CAD software program and fabricated by using 2 techniques and materials: CAD-CAM-milled (CCM) and 3D-printed (3DP).

Erratum: Construction of the plasmid for expression of EGFP-M-IL-2(88Arg, 125Ala) fusion protein and the anti-tumor effects exerted by the fusion protein in HeLa-60 cells.

[This corrects the article DOI: 10.3892/ol.2015.

Effects of low-dose rhBMP-2 on peri-implant ridge augmentation in a canine model.

Aims: The aim of this study was to investigate the potential of low-dose rhBMP-2 combined with Hydroxyapatite (HAp)/β-tricalcium phosphate (TCP)/ Collagen (Col) composite in repairing the peri-implant critical size defect and to determine the optimal rhBMP-2 concentration.

Materials And Methods: Fifty saddle-type alveolar defects (10 mm mesiodistally and 4 mm apicocoronally) were surgically prepared on edentulous ridges in ten beagle dogs. Following implant placement, the defects with vertically exposed implant fixtures either received (a) no graft, (b) HAp/TCP/Col composite, (c) HAp/TCP/Col +0.

Preliminary evaluation of BMP-2-derived peptide in repairing a peri-implant critical size defect: A canine model.

Background/purpose: A synthetic bone morphogenetic protein (BMP)-2-derived peptide has been discovered to promote bone regeneration. The present study investigated the potential of the BMP-2 peptide combined with hydroxyapatite (HAp)/β-tricalcium phosphate (TCP)/collagen (Col) composite in repairing a peri-implant critical size defect.

Methods: Twenty-four saddle-type alveolar defects (10 mm mesiodistally and 4 mm apicocoronally) were surgically prepared in edentulous ridges in four male beagle dogs.

Toxic mechanisms of Roth801, Canals, microparticles and nanoparticles of ZnO on MG-63 osteoblasts.

ZnO eugenol-based materials are widely used for restoration of caries cavity, apical retrograde filling and root canal sealer. Their effects on apical bone healing await investigation. The toxic mechanisms of ZnO particles and nanoparticles to MG-63 osteoblastic cells were studied.

Regulation of the regenerative activity of dental pulp stem cells from exfoliated deciduous teeth (SHED) of children by TGF-β1 is associated with ALK5/Smad2, TAK1, p38 and MEK/ERK signaling.

Transforming growth factor-β1 (TGF-β1) regulates wound healing/regeneration and aging processes. Dental pulp stem cells from human exfoliated deciduous teeth (SHED) are cell sources for treatment of age-related disorders. We studied the effect of TGF-β1 on SHED and related signaling.

Effects of fabrication techniques on denture base adaptation: An in vitro study.

Statement Of Problem: Information about the accuracy of maxillary and mandibular denture bases made with different fabrication techniques under uniform test conditions is lacking.

Purpose: The purpose of this in vitro study was to evaluate the denture base adaptation of computer-aided design and computer-aided manufactured (CAD-CAM) milled, 3D printed, and conventional heat-polymerized resin fabrication techniques.

Material And Methods: Maxillary and mandibular edentulous models fabricated from cobalt-chromium alloy were scanned, and 2-mm-thick denture bases were designed and fabricated by using 4 fabrication techniques and materials: CAD-CAM milled (CCM), 3D printed (3DP), injection molded (IM), and compression molded (CM).

Identification of candidate biomarkers and pathways associated with SCLC by bioinformatics analysis.

Small cell lung cancer (SCLC) is one of the highly malignant tumors and a serious threat to human health. The aim of the present study was to explore the underlying molecular mechanisms of SCLC. mRNA microarray datasets GSE6044 and GSE11969 were downloaded from Gene Expression Omnibus database, and the differentially expressed genes (DEGs) between normal lung and SCLC samples were screened using GEO2R tool.

Analyzing gene expression profiles with preliminary validations in cardiac hypertrophy induced by pressure overload.

The aim of this study was to identify the key genes involved in the cardiac hypertrophy (CH) induced by pressure overload. mRNA microarray data sets GSE5500 and GSE18801 were downloaded from the Gene Expression Omnibus database, and differentially expressed genes (DEGs) were screened using the Limma package; then, functional and pathway enrichment analysis were performed for common DEGs using the Database for Annotation, Visualization and Integrated Discovery database. Furthermore, the top DEGs were further validated using quantitative PCR in the hypertrophic heart tissue induced by isoprenaline.

Influence of Implant Number and Location on Strain Around an Implant Combined with Force Transferred to the Palate in Maxillary Overdentures.

This study evaluated the effect of implant number and location on strain around the implant and force transferred to the palate in maxillary implant overdentures (IODs), including two locators attached bilaterally in the canine region (IOD 2), four locators attached bilaterally in the canine and premolar regions (IOD 4CP), four locators attached bilaterally in the canine and molar regions (IOD 4CM), and six locators attached bilaterally in the canine, premolar, and molar regions (IOD 6). As the implant number increased, strain around the implant regions increased, whereas force transferred to the palate decreased under loading. However, the differences were small between IOD 4CM and IOD 6, suggesting identical biomechanical effectiveness.

Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells.

Aims: Butyric acid is one major metabolic product generated by anaerobic Gram-negative bacteria of periodontal and root canal infection. Butyric acid affects the activity of periodontal cells such as osteoblasts. The purposes of this study were to investigate the effects of butyrate on MG-63 osteoblasts.

Prostaglandin E2 Stimulates EP2, Adenylate Cyclase, Phospholipase C, and Intracellular Calcium Release to Mediate Cyclic Adenosine Monophosphate Production in Dental Pulp Cells.

Introduction: Prostaglandin E2 (PGE2) plays a crucial role in pulpal inflammation and repair. However, its induction of signal transduction pathways is not clear but is crucial for future control of pulpal inflammation.

Methods: Primary dental pulp cells were exposed to PGE2 and 19R-OH PGE2 (EP2 agonist) or sulprostone (EP1/EP3 agonist) for 5 to 40 minutes.

Construction of the plasmid coding for the expression of the EGFP--2(Arg, Ala) fusion protein and the anti-tumor effects exerted by the fusion protein in HeLa-60 cells.

Gene therapy is a promising therapeutic option for the treatment of various cancers, and tumor-targeted plasmids encoding toxic protein genes are potential tools for gene therapy. In the present study, a recombinant plasmid containing the genes for the toxic protein melittin and interleukin-2 (IL-2) was constructed. Melittin and IL-2 are known to play key roles in immunoregulation and cancer therapy, but they each possess defects that limit the clinical application of these proteins.

The effect of implant design and bone quality on insertion torque, resonance frequency analysis, and insertion energy during implant placement in low or low- to medium-density bone.

Purpose: This study investigated the effect of implant design and bone quality on insertion torque (IT), implant stability quotient (ISQ), and insertion energy (IE) by monitoring the continuous change in IT and ISQ while implants were inserted in artificial bone blocks that simulate bone of poor or poor-to-medium quality.

Materials And Methods: Polyurethane foam blocks (Sawbones) of 0.16 g/cm³ and 0.

Using acrylic resin tooth veneers in patients with an abnormal jaw relationship: a case report.

This case report describes and evaluates a method of arranging artificial teeth in patients with an abnormal jaw relationship in which a wider maxillary arch opposes a narrower mandibular arch. First, the fossa of mandibular posterior teeth was positioned on the crest of the mandibular edentulous ridge. The maxillary posterior teeth were then placed palatally to maximize occlusal contacts with the opposing mandibular teeth.

Antiplatelet effect of catechol is related to inhibition of cyclooxygenase, reactive oxygen species, ERK/p38 signaling and thromboxane A2 production.

Catechol (benzenediol) is present in plant-derived products, such as vegetables, fruits, coffee, tea, wine, areca nut and cigarette smoke. Because platelet dysfunction is a risk factor of cardiovascular diseases, including stroke, atherosclerosis and myocardial infarction, the purpose of this study was to evaluate the anti-platelet and anti-inflammatory effect of catechol and its mechanisms. The effects of catechol on cyclooxygenase (COX) activity, arachidonic acid (AA)-induced aggregation, thromboxane B2 (TXB2) production, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) production and extracellular signal-regulated kinase (ERK)/p38 phosphorylation were determined in rabbit platelets.