Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization.

Background: Andrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187.

Fine-tuning gene expression of regulator AdmX for improved biosynthesis of andrimid in Erwinia persicina BST187.

Andrimid is a potent antibiotic that inhibits acetyl-CoA carboxylase. However, its low biological yield and complex chemical synthesis have hindered its large-scale application. In this study, we found that the LysR-type transcriptional activator AdmX controls andrimid yield by adjusting its expression level in the andrimid-producing bacterium Erwinia persicina strain BST187.

First Report of Causing Bacterial Blackleg and Soft Rot of Potato in Pennsylvania.

Potato () plants showing blackleg and soft rot symptoms were collected at a commercial vegetable farm near Newmanstown, PA in August 2021 (Fig. S1). The incidence of potato blackleg in the unirrigated field was about 5 to 8%, but approximately 30% in the irrigated field.

Risk Evaluation of Benzovindiflupyr Resistance of Population in Maine.

causes Verticillium wilt, resulting in significant losses to potato production. Benzovindiflupyr, a succinate dehydrogenase inhibitor, effectively controls . However, frequent applications of the chemical may expedite the development of fungicide resistance in the pathogen population.

Pangenomic Analysis of Strains Related to the Outbreak of Blackleg and Soft Rot of Potato in the United States.

has caused an outbreak of blackleg and soft rot of potato in the eastern half of the United States since 2015. To investigate genetic diversity of the pathogen, a comparative analysis was conducted on genomes of strains. Whole genomes of 16 strains from the United States outbreak were assembled and compared with 16 previously sequenced genomes of isolated from potato or carnation.

Interaction between and in Potato Infection under Field Conditions.

and spp. both cause blackleg and soft rot of potato, which can be a yield-reducing factor to potato production. The purpose of this study was to examine the interaction between these two bacterial genera causing potato infection, and subsequent disease development and yield responses under field conditions.

Genotyping Causing Potato Blackleg and Soft Rot Outbreak Associated With Inoculum Geography in the United States.

An outbreak of blackleg and soft rot of potato, caused primarily by the bacterial pathogen , has resulted in significant economic losses in the northeastern United States since 2015. The spread of this seedborne disease is highly associated with seed distribution; therefore, the pathogen likely spread with seed tubers. To describe the blackleg epidemic and track inoculum origins, a total of 1,183 potato samples were collected from 11 states associated with blackleg outbreak from 2015 to 2019.

Leucine Regulates Zoosporic Germination and Infection by .

Pink rot () of potato is a major concern in many potato production regions. The pathogen produces zoospores that serve as a primary inoculum for infection. To understand how the pink rot incidence is related to pathogen population, qualitative, and quantitative chemical analyses were conducted.