Niabella digestorum sp. nov., a High Cell-Surface Hydrophobic Bacterium Isolated from Waste Digestion System.

A high cell-surface hydrophobic bacterium, strain A18, was isolated from a waste digestion system in Chaozhou, China. Cells of strain A18 were Gram-stain-positive, aerobic, non-spore-forming, non-motile, and rod-shaped. Phylogenetic analyses based on the 16S rRNA gene showed that strain A18 shared less than 94.

Species' functional traits and interactions drive nitrate-mediated sulfur-oxidizing community structure and functioning.

Understanding the processes and mechanisms governing microbial community assembly and their linkages to ecosystem functioning has long been a core issue in microbial ecology. An in-depth insight still requires combining with analyses of species' functional traits and microbial interactions. Our study showed how species' functional traits and interactions determined microbial community structure and functions by a well-controlled laboratory experiment with nitrate-mediated sulfur oxidation systems using high-throughput sequencing and culture-dependent technologies.

sp. nov., isolated from the sediment of a river in Ronggui, Foshan city, China.

A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26, was isolated from the sediment of a river in Ronggui, Foshan city, China. Strain J26 grew optimally at 0 % (w/v) NaCl, pH 6.5-7.

sp. nov. and sp. nov., isolated from landfill leachate of a domestic waste treatment plant.

Two strains designated as c1 and c7, were isolated from the landfill leachate of a domestic waste treatment plant in Huizhou City, Guangdong Province, PR China. The cells of both strains were aerobic, rod-shaped, non-motile and formed yellow colonies on Reasoner's 2A agar plates. Strain c1 grew at 10-42 °C (optimum, 30 °C), pH 4.

sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov., isolated from streams in China.

Twelve Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (CY7W, CY18W, CY22W, FT31W, FT137W, FT147W, BYS50W, BYS107W, LFS511W, LX15W, LX22W and NL8W) were isolated from streams in China. Comparisons based on 16S rRNA gene sequences indicated that these strains take species of genus as close neighbours. The reconstructed phylogenetic and phylogenomic trees also showed that these strains cluster with species of genus together.

sp. nov. and sp. nov., isolated from a mariculture fishpond.

Two Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, non-flagellated, non-spore-forming and non-motile strains (YJ13C and H41) were isolated from a mariculture fishpond in PR China. Comparisons based on 16S rRNA gene sequences indicated that YJ13C and H41 shared 16S rRNA gene sequences similarities between 92.6 and 99.

Synergistic interactions of Desulfovibrio and Petrimonas for sulfate-reduction coupling polycyclic aromatic hydrocarbon degradation.

Microbial sulfate-reduction coupling polycyclic aromatic hydrocarbon (PAH) degradation is an important process for the remediation of contaminated sediments. However, little is known about core players and their mechanisms in this process due to the complexity of PAH degradation and the large number of microorganisms involved. Here we analyzed potential core players in a black-odorous sediment using gradient-dilution culturing, isolation and genomic/metagenomic approaches.

Elevated nitrate simplifies microbial community compositions and interactions in sulfide-rich river sediments.

Excessive nitrate in water systems is prevailing and a global risk of human health. Polluted river sediments are dominated by anaerobes and often the hotspot of denitrification. So far, little is known about the ecological effects of nitrate pollution on microbial dynamics, especially those in sulfide-rich sediments.

sp. nov., isolated from freshwater sediment.

A Gram stain-positive, non-spore-forming, non-motile and rod-shaped actinomycete, strain 5221, was isolated from the sediment of a river collected at Ronggui in the Pearl River Delta, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain formed a distinct lineage within the genus and had the highest sequence similarity to Tp12 (96.7 %), followed by 2C6-41 (96.

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from a subtropical stream in PR China and proposal to reclassify as comb. nov.

Five Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (FT50W, FT80W, FT92W, FT94W and FT135W) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT50W, FT94W and FT135W take strain Sac-22, and strains FT80W and FT92W take strain DCY83 as their closest neighbour in the phylogenetic trees, respectively. The G+C contents of strains FT50W, FT80W, FT92W, FT94W and FT135W were 63.

Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China.

A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strain FT127W was isolated from a subtropical stream in China. Comparison based on 16S rRNA gene sequences showed that strain FT127W belongs to genus Massilia and shares 98.5% similarity with Massilia buxea A9 as its closest neighbor.

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from subtropical streams in China.

Six Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (FT9W, FT25W, FT26W, FT109W, FT134W and CY42W) were isolated from subtropical streams in China. Comparisons based on 16S rRNA gene sequences showed that the six strains shared similarities of less than 98.1 % with other species within the family and formed two separately distinct clades in phylogenetic trees.

sp. nov. and sp. nov., isolated from a subtropical stream in PR China.

Two Gram-stain-negative, catalase- and oxidase-positive, rod-shaped, motile strains (FT29W and FT103W) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT29W and FT103W showed 98.7 and 98.

Ciceribacter ferrooxidans sp. nov., a nitrate-reducing Fe(II)-oxidizing bacterium isolated from ferrous ion-rich sediment.

A nitrate-reducing Fe(II)-oxidizing bacterial strain, F8825, was isolated from the Fe(II)-rich sediment of an urban creek in Pearl River Delta, China. The strain was Gram-negative, facultative chemolithotrophic, facultative anaerobic, non-spore-forming, and rod-shaped with a single flagellum. Phy-logenetic analysis based on 16S rRNA gene sequencing indicated that it belongs to the genus Ciceribacter and is most closely related to C.

sp. nov., sp. nov. and sp. nov., isolated from a subtropical stream in China.

Four strains assigned the names FT13W, FT14W, FT58W and FT68W were isolated from a subtropical stream in PR China. All the strains were Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile with flagella. Comparisons based on 16S rRNA gene sequences showed that strains FT13W, FT14W, FT58W and FT68W belonged to genus and shared 16S rRNA gene similarities in the range of 98.

Ciceribacter thiooxidans sp. nov., a novel nitrate-reducing thiosulfate-oxidizing bacterium isolated from sulfide-rich anoxic sediment.

Two facultative chemolithotrophic, nitrate-reducing thiosulfate-oxidizing strains, F43b and F21, were isolated from the sulfide-rich anoxic sediment of an urban creek in Pearl River Delta, China. Both strains were Gram-negative, facultatively anaerobic, non-spore-forming and rod-shaped with a flagellum. Phylogenetic analyses of 16S rRNA genes and the thrC, recA, glnII and atpD housekeeping genes revealed that the type strain shared high sequence similarities to Ciceribacter lividus MSSRFBL1, with 98.