Genetic diversity of Flaviviridae and Rhabdoviridae EVEs in Aedes aegypti and Aedes albopictus on Hainan Island and the Leizhou Peninsula, China.

Background: Hainan Island and the Leizhou Peninsula, the southernmost part of mainland China, are areas where Aedes aegypti and Ae. albopictus are sympatric and are also high-incidence areas of dengue outbreaks in China. Many studies have suggested that Aedes endogenous viral components (EVEs) are enriched in piRNA clusters which can silence incoming viral genomes.

Spatial distribution of Japanese encephalitis cases and correlated geo-environmental factors in southern and northern counties of China.

Japanese encephalitis (JE) is a mosquito-borne disease with a spatial distribution that is linked to geo-environmental factors. The spatial distribution of JE cases and correlated geo-environmental factors were investigated in two critical counties in southern and northern China. Based on maps, enhanced thematic mapper (ETM) remote sensing datasets from Landsat and spatial datasets of JE cases, spatial distribution and spatial cluster analyses of JE cases at the village scale were performed by using the standard deviational ellipse and Ripleys K-function.

Possible potential spread of Anopheles stephensi, the Asian malaria vector.

Background: Anopheles stephensi is native to Southeast Asia and the Arabian Peninsula and has emerged as an effective and invasive malaria vector. Since invasion was reported in Djibouti in 2012, the global invasion range of An. stephensi has been expanding, and its high adaptability to the environment and the ongoing development of drug resistance have created new challenges for malaria control.

The effect of artificial light at night (ALAN) on the characteristics of diapause of Aedes albopictus.

Background: Recently, the effect of artificial light at night (ALAN) on the physiology and behavior of insects has gradually attracted the attention of researchers and has become a new research topic. Aedes albopictus is an important vector that poses a great public health risk. Further studies on the diapause of Ae.

Insecticide resistance status of Aedes aegypti in border areas of Yunnan Province.

Background: Aedes aegypti is a main vector of arboviral diseases, principally dengue, chikungunya, and Zika. Insecticides remain the most effective vector control method. Pyrethroid is the main insecticide currently used, and the long-term use of insecticides can cause mosquitoes to develop knockdown resistance.

Vector Biology and Integrated Management of Malaria Vectors in China.

Malaria is an infectious disease caused by parasites, transmitted by , , , and in China. In 2021, the disease was eliminated in China after more than 70 years of efforts implementing an integrated mosquito management strategy. This strategy comprised indoor residual spray, insecticide-treated bed nets, irrigation management, and rice-fish coculture based on an understanding of taxonomic status and ecological behaviors of vector species, in conjunction with mass drug administration and promotion of public education.

Population genetics of Aedes albopictus in the port cities of Hainan Island and Leizhou Peninsula, China.

Background: Aedes albopictus is an important vector of arboviral diseases, transmitting yellow fever, dengue fever, chikungunya and Zika. Monitoring its population genetic diversity and genetic differentiation has become essential for the control of infectious disease epidemics, especially in the functional areas of ports of entry. Population genetic monitoring of Ae.

Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection.

Background: Cell fusing agent virus (CFAV) was the first insect-specific virus to be characterized, and has been reported to negatively influence the growth of arboviruses such as dengue, Zika, and La Cross, making it a promising biocontrol agent for mosquito-borne disease prevention. Aedes aegypti Aag2 cells were naturally infected with CFAV. However, the ability of this virus to stably colonize an Ae.

Evolution of knockdown resistance () mutations of and in Hainan Island and Leizhou Peninsula, China.

Background: and are important vectors of human arboviruses, transmitting arboviral diseases such as yellow fever, dengue, chikungunya and Zika. These two mosquitoes coexist on Hainan Island and the Leizhou Peninsula in China. Over the past 40 years, the distribution of has gradually expanded in these areas, while the distribution of has declined dramatically mainly due to the ecological changes and some other factors such as heavy use of insecticide indoor based on endophagic bloodfeeding of the species.

Spatial and temporal analyses of the influences of meteorological and environmental factors on Aedes albopictus (Diptera: Culicidae) population dynamics during the peak abundance period at a city scale.

Aedes albopictus (Diptera: Culicidae) is a major vector of multiple diseases. While vaccines have been developed, preventing these Aedes-borne diseases continues to primarily depend on monitoring and controlling the vector population. Despite increasing research on the impacts of various factors on Ae.

Vector competence evaluation of mosquitoes for Tahyna virus PJ01 strain, a new in China.

Introduction: Tahyna virus (TAHV), an arbovirus of the genus , is a cause of human diseases and less studied worldwide. In this study, a new strain of TAHV was isolated from sp. mosquitoes collected in Panjin city, Liaoning province.

Transcriptome Analysis of Response to Zika Virus Infection in Two Strains with Different Vector Competence.

Zika virus (ZIKV), which is mainly transmitted by in temperate zones, can causes serious neurological disorders. However, the molecular mechanisms that influence the vector competence of for ZIKV are poorly understood. In this study, the vector competence of mosquitoes from Jinghong (JH) and Guangzhou (GZ) Cities of China were evaluated, and transcripts in the midgut and salivary gland tissues were sequenced on 10 days post-infection.

Effects of constant temperature and daily fluctuating temperature on the transovarial transmission and life cycle of infected with Zika virus.

Introduction: Numerous studies on the mosquito life cycle and transmission efficacy were performed under constant temperatures. Mosquito in wild, however, is not exposed to constant temperature but is faced with temperature variation on a daily basis.

Methods: In the present study, the mosquito life cycle and Zika virus transmission efficiency were conducted at daily fluctuating temperatures and constant temperatures.

An alternating transmission model between mice and mosquitoes for genetic study of dengue virus.

Rapidly increased incidence and prevalence of dengue virus serotype 2 (DENV-2) in recent decades highlight the need for better understanding of the selective pressures that drive genetic and phenotypic changes in this virus. We simulated the transfer of DENV-2 between human hosts and mosquito vectors by horizontally transmitting the virus between suckling mice and Aedes aegypti (Linnaeus, Diptera: Culicidae). A total of 3 cycles of alternating transmission were performed and 3 passages of virus population were harvested from the infected sucking mice.

infection in field-collected in Yunnan Province, southwestern China.

Background: is gram-negative and common intracellular bacteria, which is maternally inherited endosymbionts and could expand their propagation in host populations by means of various manipulations. Recent reports reveal the natural infection of in in Malaysia, India, Philippines, Thailand and the United States. At present, none of natural infection in has been reported in China.

Genetic Diversity and Population Genetic Structure of in the Yangtze River Basin, China.

is an indigenous primary vector of dengue and Zika viruses in China. Understanding the population spatial genetic structure, migration, and gene flow of vector species is critical to effectively preventing and controlling vector-borne diseases. The genetic variation and population structure of populations collected from 22 cities along the Yangtze River Basin were investigated with nine microsatellite loci and the mitochondrial gene.

Replication and transmission of West Nile virus in simulated overwintering adults of Culex pipiens pallens (Diptera: Culicidae) in China.

West Nile virus is perhaps the widest distributed arbovirus globally, being maintained in nature by transmission among ornithophagic Culex mosquitoes and a wide-variety of birds. A factor contributing to the success of the WNV invasion is the ability of the virus to successfully overwinter. Experimental studies on the preservation and transmission of West Nile virus by Culex pipiens pallens under simulated overwintering conditions have shown that Cx.