Three in one: A multifunctional oxidase-mimicking Ag/MnO nanozyme for colorimetric determination, precise identification, and broad-spectrum inactivation of foodborne pathogenic bacteria.

A multifunctional oxidase-mimicking Ag/MnO was prepared, catalyzing the 3, 3', 5, 5'-tetramethylbenzidine (TMB) chromogenic reaction. Six foodborne pathogenic bacteria species, including Escherichia coli, Staphylococcus aureus, Salmonella enterica, Listeria monocytogenes, Bacillus cereus, and Cronobacter sakazakii, were observed to differentially inhibit its oxidase-like activity, resulting in decelerating the TMB chromogenic reaction. Owing to these properties, the following achievements were achieved: colorimetric determination of these bacteria with high sensitivity can be achieved using Ag/MnO + TMB reaction system; precise identification of these bacteria at different concentrations, including individual bacterium, binary mixtures, and even multivariate mixtures, can be effectively realized by combining the Ag/MnO-based colorimetric sensor array with principal component analysis (PCA); broad-spectrum inactivation of these bacteria can be remarkably realized through catalyzation of Ag/MnO to generate superoxide anion free radicals.

Colorimetric detection of Staphylococcus aureus with enhanced sensitivity based on phage covalently immobilized CoO nanozyme through synergistic inhibition effect.

Staphylococcus aureus (S. aureus) is a common foodborne pathogen, posing a serious threat to public health. Consequently, it is crucial to establish a platform for sensitive and specific determination of S.

Quantifying HONO Production from Nitrate Photolysis in a Polluted Atmosphere.

The photolysis of particulate nitrate (pNO) has been suggested to be an important source of nitrous acid (HONO) in the troposphere. However, determining the photolysis rate constant of pNO () suffers from high uncertainty. Prior laboratory measurements of using aerosol filters have been complicated by the "shadow effect"─a phenomenon of light extinction within aerosol layers that potentially skews these measurements.

Biomechanical investigation of a customized interspinous spacer system in the treatment of degenerative disc diseases: A finite element analysis.

Background: A novel interspinous fixation system based on anatomical parameters and incorporating transfacetopedicular screws, was developed to treat degenerative disc diseases. The biomechanical characteristics of the novel system were evaluated using finite element analysis in comparison to other classical interspinous spacers.

Methods: The L1-S1 lumbar spine finite element models were surgically implanted with the novel system, Coflex and DIAM devices at the L4/L5 segment to assess the range of motion, the pression distribution of intervertebral disc, the peak stresses on the spinous process and implant during various motions.

Immobilization of a broad host range phage on the peroxidase-like Fe-MOF for colorimetric determination of multiple Salmonella enterica strains in food.

A broad host range phage-based nanozyme (Fe-MOF@SalmpYZU47) was prepared for colorimetric detection of multiple Salmonella enterica strains. The isolation of a broad host range phage (SalmpYZU47) capable of infecting multiple S. enterica strains was achieved.

Coffee and the risk of osteoarthritis: a two-sample, two-step multivariable Mendelian randomization study.

To investigate the potential causal relationship between coffee consumption and osteoarthritis (OA), and to disentangle whether body mass index (BMI) and Bone mineral density (BMD) mediate this relationship. We performed two-sample and two-step Mendelian randomization (MR) analyses utilizing publicly available genome-wide association studies (GWAS) summary statistics to estimate the association between coffee intake and OA risk (including knee OA, hip OA, knee or hip OA, and total OA), as well as the possible mediating effects of BMI and BMD. In addition, data of different coffee types (decaffeinated coffee, instant coffee, ground coffee-including espresso, filter, etc.

Insights into PM pollution of four small and medium-sized cities in Chinese representative regions: Chemical compositions, sources and health risks.

Fine particles (PM) pollution is still a severe issue in some cities in China, where the chemical characteristics of PM remain unclear due to limited studies there. Herein, we focused on PM pollution in small and medium-sized cities in key urban agglomerations and conducted a comprehensive study on the PM chemical characteristics, sources, and health risks. In the autumn and winter of 2019-2020, PM samples were collected simultaneously in four small and medium-sized cities in four key regions: Dingzhou (Beijing-Tianjin-Hebei region), Weinan (Fenwei Plain region), Fukang (Northern Slope of the Tianshan Mountain region), and Bozhou (Yangtze River Delta region).

The impact of thyroid function on total spine bone mineral density in postmenopausal women.

Purpose: Osteoporosis has been a widespread concern for older women, especially postmenopausal women. Thyroid function is crucial for bone metabolism. However, the relationship between thyroid function variation within thyroxine reference range and bone mineral density (BMD) remains ambiguous.

Development and validation of a semi-supervised deep learning model for automatic retinopathy of prematurity staging.

Retinopathy of prematurity (ROP) is currently one of the leading causes of infant blindness worldwide. Recently significant progress has been made in deep learning-based computer-aided diagnostic methods. However, deep learning often requires a large amount of annotated data for model optimization, but this requires long hours of effort by experienced doctors in clinical scenarios.

Enhanced osteogenicity of the demineralized bone-dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules.

Allogenic demineralized bone matrix (DBM), processed to expose bioactive proteins imbedded by calcium salts, is widely used for bone repair and regeneration as an alternative to the autologous bone graft. However, demineralized bone matrices from tissue banks vary significantly in residual calcium content and osteogenicity for clinical bone regeneration. The present study produced DBM with various residual calcium contents by partial demineralization using ethylenediaminetetraacetic acid disodium (EDTA) and hydrochloric acid.

Physiochemistry and sources of individual particles in response to intensified controls during the 2022 Winter Olympics in Beijing.

To investigate the particle sources before, during, and after the 2022 Beijing Winter Olympic and Paralympic (WOP) in Beijing, ambient particles were passively collected from January to March 2022. The physicochemical properties including morphology, size, shape parameters, and elemental compositions were analyzed by the IntelliSEM EPAS (an advanced computer-controlled scanning electron microscopy [CCSEM] system). Using the user-defined classification rules, 37,174 individual particles were automatically classified into 27 major groups and further attributed to seven major sources based on the source-associated characteristics, including mineral dust, secondary aerosol, combustion/industry, carbonaceous particles, salt-related particles, biological particles, and fiber particles.

Effect of vault on predicting postoperative refractive error for posterior chamber phakic intraocular lens based on a machine learning model.

Purpose: To investigate how vault and other biometric variations affect postoperative refractive error of implantable collamer lenses (ICLs) by integrating artificial intelligence and modified vergence formula.

Setting: Eye and ENT Hospital of Fudan University, Shanghai, China.

Design: Artificial intelligence and big data-based prediction model.

Biomechanical effects of osteoporosis severity on the occurrence of proximal junctional kyphosis following long-segment posterior thoracolumbar fusion.

Background: Proximal junctional kyphosis is a common long-term complication in adult spinal deformity surgery that involves long-segment posterior spinal fusion. However, the underlying biomechanical mechanisms of the impact of osteoporosis on proximal junctional kyphosis remain unclear. The present study was to evaluate adjacent segment degeneration and spine mechanical instability in osteoporotic patients who underwent long-segment posterior thoracolumbar fusion.

Association between physical activity and bone mineral density in postmenopausal women: a cross-sectional study from the NHANES 2007-2018.

Background: Physical activity (PA) is generally encouraged for the treatment of osteoporosis. However, epidemiological statistics on the level of physical activity required for bone health are scarce. The purpose of this research was to analyze the association between PA and total spine bone mineral density (BMD) in postmenopausal women.

[Short- and mid-term effectiveness of unicompartmental knee arthroplasty for post-traumatic arthritis of knee].

Objective: To investigate the short- and mid-term effectiveness of unicompartmental knee arthroplasty (UKA) for post-traumatic arthritis (PTA) of knee.

Methods: The clinical data of 30 patients with PTA of unilateral knee between March 2014 and September 2021 was retrospectively analyzed. There were 14 males and 16 females with an average of 64.

Diane-35 and metformin therapy in rats with endometrial lesions induced by dihydrotestosterone exposure.

Background: Cotreatment with metformin and Diane-35 is conventionally used in the clinic to ameliorate ovulatory dysfunction and insulin resistance in women with polycystic ovary syndrome (PCOS). We previously showed that this combination treatment could reverse endometrial hyperplasia and endometrial cancer (EC) in patients with PCOS. Here, we aimed to investigate the influence of dihydrotestosterone (DHT) and this cotreatment on the endometrium, along with the related mechanisms.

A standardized technique for lateral unicompartmental knee arthroplasty.

Objectives: We developed a new standardized procedure for lateral unicompartmental knee arthroplasty (UKA) to maximize the benefits and minimize the risks of surgery in patients with osteoarthritis (OA).

Methods: This retrospective study recruited patients who underwent lateral UKA at our hospital between January 2014 and January 2016. Demographic characteristics and preoperative and postoperative American Knee Society (AKS) clinical scores (including pain, clinical, and knee mobility scores) were collected.

Alexithymia in a Chinese Patient with Chronic Pain and Associated Factors: A Cross-Sectional Study.

Background: Alexithymia is more prevalent among those with patients living with chronic pain. Information on the prevalence of alexithymia in Chinese patients with chronic pain and associated factors is limited.

Aim: The primary objective of this study was to determine the prevalence of alexithymia, as defined by a score of 61 or greater in the 20-item Toronto Alexithymia Scale (TAS-20), in a Chinese patient with chronic pain.

Non-invasive high frequency oscillatory ventilation inhibiting respiratory motion in healthy volunteers.

Precision radiotherapy needs to manage organ movements to prevent critical organ injury. The purpose of this study is to examine the feasibility of motion control of the lung by suppressing respiratory motion. The non-invasive high frequency oscillatory ventilation (NIHFOV) is a technique commonly used in the protection of lung for patients with acute lung disease.

Artificial Intelligence Model for Antiinterference Cataract Automatic Diagnosis: A Diagnostic Accuracy Study.

Cataract is the leading cause of blindness worldwide. In order to achieve large-scale cataract screening and remarkable performance, several studies have applied artificial intelligence (AI) to cataract detection based on fundus images. However, the fundus images they used are original from normal optical circumstances, which is less impractical due to the existence of poor-quality fundus images for inappropriate optical conditions in actual scenarios.

Pain catastrophizing and associated factors in preoperative total knee arthroplasty in Lanzhou, China: a cross-sectional study.

Background: Pain catastrophizing in preoperative total knee arthroplasty (TKA) patients is associated with several poorly characterised factors in the literature. This study investigated the current state and associated factors of preoperative pain catastrophizing in patients undergoing TKA.

Methods: This descriptive cross-sectional study was conducted at the orthopedics ward of two tertiary hospitals in Lanzhou, China.