miR-374-5p inhibits osteogenesis by targeting PTEN/PI3K/AKT signaling pathway.

Purpose: This study aims to evaluate the effects of miR-374-5p on osteogenesis in rat osteoblasts, validate its target on PTEN, and explore its role in the PTEN/PI3K/AKT signaling pathway during osteoblast differentiation.

Methods: We transfected 293T cells with miR-374-5p mimics and inhibitors, followed by Western blot and qRT-PCR analyses to assess protein and mRNA expression levels. A dual-luciferase assay was performed to confirm direct targeting.

Protective and risk factors in daily life associated with cognitive decline of older adults.

Background: Cognitive decline is a chronic condition which is characterized by a loss of the ability to remember, learn, and pay attention to complex tasks. Many older people are now suffering from cognitive decline, which decreases life quality and leads to disability. This study aimed to identify the risk and protective factors for cognitive decline of the older people from daily life and establish a predictive model using logistic regression.

Impact of Breastfeeding Practices on Autistic Traits in Chinese Children Aged from 3 to 4 Years: Cross-Sectional Study.

: Although breastfeeding has been extensively documented to confer health benefits to infants in the early stages of their lives, the sustained influence that it has on autistic traits throughout childhood remains unclear. This study endeavors to explore the correlation between the length of exclusive breastfeeding, the overall duration of breastfeeding, and the manifestation of autistic traits in Chinese children. : A cross-sectional study was conducted among 17,382 three-year-olds residing in Longhua District, Shenzhen, China.

Multiprobe Photoproximity Labeling of the EGFR Interactome in Glioblastoma Using Red-Light.

Photocatalytic proximity labeling has emerged as a valuable technique for studying interactions between biomolecules in a cellular context, providing precise spatiotemporal control over protein labeling. One significant advantage of these methods is their modularity, allowing the use of a single photocatalyst with different reactive probes to expand interactome coverage and capture diverse protein interactions. Despite these advances, fewer methods have been developed using red-light excitation, limiting the use of photoproximity labeling in more complex media such as tissues and animal models.

Relacorilant plus nab-paclitaxel for recurrent, platinum-resistant ovarian cancer: a cost-effectiveness study.

Objective: The phase 2, open-label, randomized, 3-arm study (NCT03776812) found that intermittently dosed relacorilant + nab-paclitaxel improved progression-free survival, duration of response, and overall survival compared with nab-paclitaxel monotherapy with minimal additional toxicity. This study analyzed the cost-effectiveness of intermittent relacorilant + nab-paclitaxel (IN), continuous relacorilant + nab-paclitaxel (CN) and nab-paclitaxel monotherapy (N) from the payer's perspective over a 5-year time horizon.

Methods: The health outcome is expressed in quality-adjusted life years (QALYs).

Virtual library docking for cannabinoid-1 receptor agonists with reduced side effects.

Virtual library docking can reveal unexpected chemotypes that complement the structures of biological targets. Seeking agonists for the cannabinoid-1 receptor (CB1R), we dock 74 million tangible molecules and prioritize 46 high ranking ones for de novo synthesis and testing. Nine are active by radioligand competition, a 20% hit-rate.

Immunosuppressed patient with suspected systemic infection: A case report.

Fungal infections are a significant cause of systemic infections in immunosuppressed patients. Studies indicate that the most prevalent fungal pathogens include (25 %), (25 %), and (25 %) [1]. , a lipophilic, opportunistic pathogenic yeast, primarily causes skin infections, but there are increasing reports of invasive infections.

Glymphatic system: a self-purification circulation in brain.

The glymphatic system theory introduces a new perspective on fluid flow and homeostasis in the brain. Here, cerebrospinal fluid and interstitial fluid (CSF-ISF) moves from the perivascular spaces (PVS) of arteries to those of veins for drainage. Aquaporin-4 (AQP4) plays a crucial role in driving fluid within the PVS.

Comparison of different approaches applied for surgical correction of partial anomalous pulmonary venous connection.

Background: Right anterolateral thoracotomy or left anterolateral thoracotomy applied to partial anomalous pulmonary venous connection correction have been reported to obtain cosmetic and less invasive outcomes as alternative approaches to median sternotomy. However, the application of different approaches is still confusing. We compared the perioperative and mid-term outcomes to seek indications of different approaches and guide surgical treatment of partial anomalous pulmonary venous connection.

FeO-Based Nanospheres with High Photothermal Conversion Efficiency for Dual-Effect and Mild Biofilm Eradication against Periodontitis.

Periodontitis, a chronic inflammatory oral disease resulting from plaque biofilms, affects about 743 million individuals worldwide. However, the efficacy of current treatments is hampered by challenges in delivering antibiotics to recalcitrant oral biofilms and bacterial resistance, thereby impeding successful treatment of infectious diseases. To address the issues, an antibacterial photothermal material was designed, comprising a spherical structure of zinc oxide (ZnO) wrapped with triiron tetraoxide (FeO).

Histone acetyltransferase BmMOF inhibits the proliferation of the Bombyx mori nucleopolyhedrovirus by targeting Bmp53.

Bombyx mori nucleopolyhedrovirus (BmNPV) infection is a critical disease in silkworms (Bombyx mori), yet the molecular mechanism underlying the defense of Bombyx mori against BmNPV infection remains elusive. The histone acetyltransferase MOF plays a crucial role in cellular stress response and apoptosis. Nevertheless, its function in Bombyx mori is yet to be fully elucidated.

A "three-in-one" detection platform based on cerium-coated metal-organic framework for multimodal glyphosate detection.

Glyphosate (Glyp) is a widely used herbicide, but its through prolonged and excessive spraying residues pose significant health risks. A ratiometric multimodal sensing platform combining fluorescence, colorimetry, and photothermal detection was developed for Glyp detection using a cerium/polyacrylic acid-modified porphyrin-based metal-organic framework (PCN@PAA-Ce). The detection strategy relies on the inhibition of acetylcholinesterase by Glyp, the oxidase-like activity of PCN@PAA-Ce, and the competition between enzymatic products and 3,3',5,5'-tetramethylbenzidine (TMB).

Mosquito mutations F290V and F331W expressed in acetylcholinesterase of the sand fly Phlebotomus papatasi (Scopoli): biochemical properties and inhibitor sensitivity.

Background: The Old World sand fly, Phlebotomus papatasi (Scopoli), a vector of zoonotic cutaneous leishmaniasis, is usually controlled by insecticides, including anticholinesterases. Previous studies have revealed 85% amino acid sequence identity of recombinant P. papatasi acetylcholinesterase (rPpAChE1) to mosquito AChE.

Epidemiology of Maternal Hypertensive Disorders.

Background: Maternal hypertensive disorders during pregnancy are a worldwide health problem, particularly in the countries/regions with low sociodemographic levels. This study aimed to reveal and predict the hypertensive disorders during pregnancy-related epidemiological trends.

Methods: Using data from the Global Burden of Disease 2019 study, we constructed an age-period-cohort model to assess the net drift (annual percentage changes), associated age, period, and cohort effects across global and different sociodemographic index (SDI) regions.

Associations of waist circumference and BMI with the trajectory of cardiometabolic multimorbidity in hypertensive patients: A multi-state model.

Background And Aims: The burden of cardiometabolic multimorbidity (CMM) in hypertensive patients is substantial, and obesity may play an important role in progression of CMM. We aim to explore associations of obesity with the transition patterns from hypertension to first cardiometabolic disease (FCMD), CMM, and death.

Methods And Results: 21 286 hypertensive patients over 40 were enrolled in Yinzhou Health Information System from 2010 to 2015, followed until June 30, 2022.

[Risk prediction of Reduning Injection batches by near-infrared spectroscopy combined with multiple machine learning algorithms].

In this paper, near-infrared spectroscopy(NIRS) was employed to analyze 129 batches of commercial products of Reduning Injection. The batch reporting rate was estimated according to the report of Reduning Injection in the direct adverse drug reaction(ADR) reporting system of the drug marketing authorization holder of the Center for Drug Reevaluation of the National Medical Products Administration(National Center for ADR Monitoring) from August 2021 to August 2022. According to the batch reporting rate, the samples of Reduning Injection were classified into those with potential risks and those being safe.

Multiple independent acquisitions of ACE2 usage in MERS-related coronaviruses.

The angiotensin-converting enzyme 2 (ACE2) receptor is shared by various coronaviruses with distinct receptor-binding domain (RBD) architectures, yet our understanding of these convergent acquisition events remains elusive. Here, we report that two bat MERS-related coronaviruses (MERSr-CoVs) infecting Pipistrellus nathusii (P.nat)-MOW15-22 and PnNL2018B-use ACE2 as their receptor, with narrow ortholog specificity.

Comparison of bullying victimization and depression symptoms among Chinese adolescents before and after the end of the dynamic zero-COVID-19 policy: a repeated cross-sectional study.

Background: The COVID-19 pandemic has had a significant impact on the physical and mental health of human beings. Most adolescents in mainland of China have been infected with COVID-19 after the adjustment of the dynamic zero-COVID-19 policy. This study aims to analyze the relationship between the COVID-19 pandemic effect and depressive symptoms among adolescents in mainland China.

A three-dimensional marine plastic litter real-time detection embedded system based on deep learning.

Marine plastic pollution has emerged as a significant ecological and biological issue impacting global marine ecosystems. To develop real-time cleaning systems for marine plastic litter, we implemented a three-dimensional marine plastic litter real-time detection (3D-MPLRD) system that incorporates deep learning techniques. Specifically, image quality assessment and enhancement are applied to mitigate the adverse effects of harsh underwater conditions on image quality, thereby enhancing the training efficacy of models beyond that achieved by conventional approaches.

Difference of SARS-CoV-2 infection and influence factors between people with and without HIV infection.

Background: There are mixed findings in the literature regarding the association between HIV status and the risk of COVID-19 infection. Thus, we aimed to estimate the association between characteristics of HIV infection and the risk of COVID-19 Infection in a Chinese sample.

Methods: We conducted a cross-sectional survey of 1995 people living with HIV (PLWH) and 3503 HIV-negative adults in Ningbo, China.

TTC7A Variants Results in Gastrointestinal Defects and Immunodeficiency Syndrome: Case Series and Literature Review.

Gastrointestinal Defects and Immunodeficiency Syndrome-1 (GIDID-1), caused by abnormalities in TTC7A, is an autosomal recessive disorder characterized by multiple gastrointestinal malformations and immune deficiencies, often accompanied by inflammatory bowel disease (IBD). This condition typically results in poor treatment outcomes and is usually fatal in early infancy. This paper examined the genetic abnormalities and clinical features of GIDID by analyzing data from three children and one fetus with gastrointestinal dysfunction and immune deficiency associated with TTC7A abnormalities at our hospital, and reviewed reported cases worldwide.

CO2 field-flooding devices offer potential value for cardiopulmonary bypass procedures for CHD performed via a right-side small incision approach.

Objective: This study sought to evaluate the value of a CO field-flooding device in cardiopulmonary bypass (CPB) surgical procedures for congenital heart disease (CHD) performed via a right-side small incision approach.

Methods: Between April 2022 and December 2023, 234 children with simple CHD who underwent CPB via a right-side small incision approach were separated into a control group (n = 93) without the use of a CO field-flooding device and a treatment group (n = 141) in which this device was added to the traditional surgical manual exhaust. Demographic, perioperative, arterial blood gas (ABG), and laboratory test data were then compared between these groups of patients.

Single-Cell Insights Into Cellular Response in Abdominal Aortic Occlusion-Induced Hippocampal Injury.

Objective: Ischemia-reperfusion of the abdominal aorta often results in damage to distant organs, such as the heart and brain. This cellular heterogeneity within affected tissues complicates the roles of specific cell subsets in abdominal aorta occlusion model (AAO) injury. However, cell type-specific molecular pathology in the hippocampus after ischemia is poorly understood.

Bioinspired Tunable Helical Fiber-Shaped Strain Sensor with Sensing Controllability for the Rehabilitation of Hemiplegic Patients.

Fiber-based strain sensors, as wearable integrated devices, have shown substantial promise in health monitoring. However, current sensors suffer from limited tunability in sensing performance, constraining their adaptability to diverse human motions. Drawing inspiration from the structure of the spiranthes sinensis, this study introduces a unique textile wrapping technique to coil flexible silver (Ag) yarn around the surface of multifilament elastic polyurethane (PU), thereby constructing a helical structure fiber-based strain sensor.