To monitor radiocesium activity in skeletal muscle of live cattle, the animals were given radiocesium-contaminated feed continuously, then switched to contamination-free feed after radiocecium concentration in peripheral blood (PB) reached plateau. Radioactivity in skeletal muscles of neck and rump was measured by attaching the probe of a NaI survey meter closely on the body surface just above the muscle of the live cattle (external measurement). We validated the strong positive correlation between the value of the external measurement and radiocesium activity concentration of dissected muscle (r = 0.
View Article and Find Full Text PDFObjective: To determine the anti-inflammatory effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with coagulase-negative staphylococci (CNS).
Animals: 12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis.
Procedure: Clinical signs, number of bacteria in milk, somatic cell count (SCC) in milk, concentrations of alpha-lactalbumin and lactoferrin in milk, and concentration of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials (6 cows).
To evaluate the clinical effects of bovine lactoferrin on staphylococcal mastitis in Holstein cows during the early non-lactating period, 41 mammary quarters were selected randomly from 36 cows on 3 dairy farms. Twelve quarters were infused intramammarily with bovine lactoferrin. Twenty-nine quarters were infused with antibiotic as a control.
View Article and Find Full Text PDFLactoferrin (Lf) may play a key role in the clearance of microorganisms from a host. To study in vitro the bactericidal mechanisms of Lf during nonlactating periods, we investigated whether the effects of Lf were influenced by bovine mammary gland secretory cells (MGSC) and fresh normal bovine serum (NBS) as a source of complement. Phagocytic killing tests demonstrated that a phagocytic mixture of unopsonized Staphylococcus aureus (S.
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