Publications by authors named "Tomoyuki Kozutsumi"

Article Synopsis
  • Mastitis is a common issue in dairy cows that leads to lower milk production and quality, but probiotics like Bacillus subtilis C-3102 may help prevent it instead of using antibiotics.
  • Research showed that feeding cows this probiotic reduced the occurrence of mastitis, decreased medication days, and minimized milk disposal time while keeping somatic cell counts lower than the control group.
  • Additionally, probiotic treatment appeared to lower stress hormone levels and improve immune cell responses, suggesting B. subtilis C-3102 is effective in preventing mastitis in cows.
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To monitor radiocesium activity in skeletal muscle of live cattle, the animals were given radiocesium-contaminated feed continuously, then switched to contamination-free feed after radiocecium concentration in peripheral blood (PB) reached plateau. Radioactivity in skeletal muscles of neck and rump was measured by attaching the probe of a NaI survey meter closely on the body surface just above the muscle of the live cattle (external measurement). We validated the strong positive correlation between the value of the external measurement and radiocesium activity concentration of dissected muscle (r = 0.

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Objective: To determine the anti-inflammatory effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with coagulase-negative staphylococci (CNS).

Animals: 12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis.

Procedure: Clinical signs, number of bacteria in milk, somatic cell count (SCC) in milk, concentrations of alpha-lactalbumin and lactoferrin in milk, and concentration of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials (6 cows).

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To evaluate the clinical effects of bovine lactoferrin on staphylococcal mastitis in Holstein cows during the early non-lactating period, 41 mammary quarters were selected randomly from 36 cows on 3 dairy farms. Twelve quarters were infused intramammarily with bovine lactoferrin. Twenty-nine quarters were infused with antibiotic as a control.

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Lactoferrin (Lf) may play a key role in the clearance of microorganisms from a host. To study in vitro the bactericidal mechanisms of Lf during nonlactating periods, we investigated whether the effects of Lf were influenced by bovine mammary gland secretory cells (MGSC) and fresh normal bovine serum (NBS) as a source of complement. Phagocytic killing tests demonstrated that a phagocytic mixture of unopsonized Staphylococcus aureus (S.

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