Screening of an α-amylase inhibitor peptide by photolinker-peptide array.

Peptide arrays in which peptides were immobilized on cellulose membranes through photolinkers were synthesized. The peptides were subsequently detached from the arrays by ultraviolet (UV) photolysis for 3 h, and were used to search for functional peptides that inhibit the activity of α-amylase derived from human pancreatic juice. Amino acid replacement with high-molecular-size amino acids, Arg (R), Phe (F), Trp (W), or Tyr (Y), for the first and seventh residues of amylase inhibitor peptide, GHWYYRCW, as previous reported, led to enhancement of the inhibitory effect of the peptide on α-amylase.

Peptide array-based characterization and design of ZnO-high affinity peptides.

Peptides with both an affinity for ZnO and the ability to generate ZnO nanoparticles have attracted attention for the self-assembly and templating of nanoscale building blocks under ambient conditions with compositional uniformity. In this study, we have analyzed the specific binding sites of the ZnO-binding peptide, EAHVMHKVAPRP, which was identified using a phage display peptide library. The peptide binding assay against ZnO nanoparticles was performed using peptides synthesized on a cellulose membrane using the spot method.

Screening of peptides with a high affinity for ZnO using spot-synthesized peptide arrays and computational analysis.

Metal-binding peptides have attracted attention for their usefulness in biomineralization processes. In this work screening of high affinity peptides against ZnO was performed using a combinatorial library approach with a peptide array and computational analysis. The computationally assisted peptide screening and design enabled identification of linear peptides with affinity for ZnO with limited experimentation.

An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle: comparison between cell synchronization methods.

Background: A novel high-throughput analysis, cell array system, was developed for an extensive study of the expression of genes and/or the degradation of gene products at the cellular level. To exemplify the usefulness of this system, we showed the changes in the expression level of cyclin A and B1 during the cell cycle in a single experiment.

Methods: We used the cell array system to chase the changes in cyclin A and B1 expression during the cell cycle in HeLa cells.

Efficient 340-nm light generation by a ridge-type waveguide in a first-order periodically poled MgO:LiNbO3.

Quasi-phase-matched (QPM) UV second-harmonic generation (SHG) in a periodically poled MgO:LiNbO3 waveguide is presented. A ridge-type waveguide with high nonlinearity and strong resistance to photorefractive damage was achieved by use of an ultraprecision machining technique. By use of this waveguide in 1.

Generation of 360-nm ultraviolet light in first-order periodically poled bulk MgO:LiNbO3.

Ultraviolet second-harmonic generation in first-order periodically poled MgO:LiNbO3 is presented. Using a high-voltage multipulse application method, we fabricated a first-order nonlinear grating with a period of 1.8 microm and depth of approximately 150 microm over 10-mm interaction length in a 1-mm-thick MgO:LiNbO3 substrate.