Development of Dembo PCR for cattle in accordance with Japanese livestock quarantine guidelines.

Detection of microorganisms from bovine using real-time PCR (Dembo PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood.