Purification, Characterization, and Gene Cloning of a Cold-Adapted Endo-1,4-β-glucanase from Bellamya chinensis laeta.

An endo-1,4-β-glucanase from Bellamya chinensis laeta was purified to electrophoretically homogeneous state. The molecular weight of the purified enzyme was estimated 70,000 by SDS-PAGE. The enzyme was most active at pH 5.