Objective: To develop radioimmunoassay for aquaporin-9 (AQP9) and search for its presence in certain rat tissues.
Methods: Anti-AQP9 antiserum has been raised in New Zealand white rabbits immunized with a conjugate of synthetic AQP9 with bovine serum albumin. Radioiodination of AQP9 was performed by chloramin T method followed by purification of radioiodinated material on Sephadex G-25 column.